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The method takes microcrystalline cellulose as affinity column chromatographic medium of Beta-glucosaccharase; under low temperature and anacid condition, the sample of Beta-glucosaccharase is loaded and specifically adsorbed to the microcrystalline column, and then the concentration of NaCl is increased to elute the enzyme, so as to achieve high-efficient and economic separation and purification.

本方法以微晶纤维素作为β-葡萄糖苷酶的亲和柱层析介质，在低温和微酸性条件下，将β-葡萄糖苷酶上样并特异性吸附于微晶纤维素柱，然后再通过增加NaCl浓度的方法将该酶洗脱，从而达到其高效而经济分离纯化的目的。

The main concern of this dissertation is the relations between republicanism and modern political society. It demonstrates that republican commitment is the legitimacy of modern politics, which means that all members of a civic community share the political sovereignty and civic community should put more emphasize collective self-rule, but both dynamics of modern society and cotemporary dominating political social and moral language of liberalism go against the republicanism's commitment, so this dissertation explores that the civic community should renew the common good, regenerate the deliberative public sphere and foster civic virtue so as to remedy the maladies of modern society.

本文的核心问题是共和主义与现代政治的相关性，主要论点是现代政治的正当性基础在于共和政治的公民共同体全体成员共同享有政治主权，也即公民共同体集体自治原则，但是现代政治一方面是建立在对共和政治的规范性承诺基础之上，另一方面现代社会的内在动力以及现代社会的主流政治社会道德话语对于共和政治的实现和维持或者存在威胁，或者隐含缺陷，要维持和实现共和政治的规范性承诺，需要恢复公共善的优先性、重建协商的公共领域以及塑造公民德性。

According to Zhou Yang, He Qi-fang, Ding Ling as the particular objects, my article describes their processes in thinking conversion and internal logic routes from the angel of the writers principle part. I will analyze the"incline"to the direction of the great frame of political ideology so as to dialyze their cultural puzzles, hardship choices and cultural diseases existed in themselves.

文章又以周扬、何其芳、丁玲为具体对象，从文人主体的角度，描述他们思想转变的过程与内在理路，分析其文学理论与创作成果向政治意识形态方向的&倾斜&和在政治意识形态大框架中闪出的重重&缝隙&，以此透析文人进入政治体制的运作轨道后所面临的文化困境、艰难选择以及自身存在的文化病症。

The wave is the core problem of low Fr. current .In order to make diffluent piers with low ridge to be popularized and applied, the surface wave of low Fr. current is chosen as the research object .We measure the surface wave elements and the velocity distribution of various diffluent piers scheme by the experiment of physical model. We also analyze the average wave height, the the average value of the square of wave , unit width of the waves energy , the probability density distribution of wave height ,and the power chart character, so as to reveal the weakening wave mechanism of the diffluent piers .

低Fr水流的核心是波浪问题，为了低坎分流墩的推广应用，本研究以低Fr水流的表面波浪作为研究对象，通过物理模型试验，对各种分流墩方案消能工的下游水面波浪要素、流速分布进行测试，分析低坎分流墩下游波浪的平均波高、均方根、单宽波能、波高概率密度分布及功率谱特性变化规律，从而揭示了低坎分流墩的削波机理。

As the construction of the administrative rule of law is relatively late in our country, it is of great significance for our country lacking in law tradition to standardize and control the administration discretional power and make its operation meet the needs of socialist market economy so as to serve the modernization better in the course of transformation period.

行政法治建设在我国起步较晚，在社会转型过程中，如何规范、控制行政自由裁量权，使行政自由裁量权的运行能够符合社会主义市场经济的需要，更好地为现代化建设服务，在我们这个缺乏法治传统的国家尤其具有现实意义。

There were some reasons why the empire had gone to decline as follow:founding a state by the military so as to its foundation being unstable; studying from the west being an action of catching up with and surpassing something equidistantly; culture and education lagging behind and lacking the delayed effe.

帝国之走向衰落的原因是：靠军事立国，大厦根基不稳；向西方学习，是一种等距离赶超；文化教育落后，缺乏发展后劲；专制官僚制度日益腐败，问题严重积重难返；调节机制不良，后期改革拾级而下。

High elastic etherate is applied to foam on the spot in normal temperature and normal pressure,so as to form the foam whose inner surface is the same as that of the jade craftwork and whose foaming ratio is high.The profile is cutted by laser and its surface is electrostaticly woolimplanted.

采用高回弹组合醚在常温常压下现场发泡，成型出与玉器工艺品外形吻合、发泡倍率较高的泡沫体，用激光刀剖切并对剖切面及成型轮廓面进行静电植绒，做出了外形美观、减震效果优异的高档内包装。

Given that almost any mage will evocate at the last sec or so of IV so as to have a faster evo and have it uninterrupted by the mass amounts of raid damage.

在raid中它们一样超级有用，几乎所有法师都会在冰纹的最后一秒来唤醒，而且其伤害不可影响施法的特性对输出也有巨大帮助。

Because of many accounts of the history, talking about the traditional filial piety, the modern men see it as the feudal feculent so as to ask for absolutely rejection the filial piety. But according to my exploration, I think the traditional filial piety has some shortages, but also it has many faultless parts, if we can be fit to use, it still has some price.

笔者经过考察，认为中国传统孝道虽然在某些方面起到了扼杀人性，阻碍社会发展的作用，但就其自身言，有着深厚的历史基础与自己独特而又丰富的内涵，对中国古代社会也有着诸多的积极意义，直至现代如若加以适当的应用，依然存有一定的价值。

In macro-perspective views, the popularity of codeswitching in ads reveals people"s psychological tendencies of westernization worship and language superiority admiration; in terms of ads plotting, code choice is usually employed as an effective strategy to call people"s attention so as to fulfill the advertising tasks. In addition, Markedness theory further explains how copywriters use implicatory meanings a code itself conveys to affect the target audience"s understanding of copywriters" purposes and intentions.

从宏观的角度上看，中―英语码转换在中国媒体广告中的流行趋势从心理学的角度体现了人们崇洋的心理及对&优势语言&的崇拜；从广告的创作方面来说，语码的选择是广告撰稿人为了引起顾客的注意从而达到广告宣传目的所采用的一种有效手段；&有标记理论&则从语言学的角度分析了广告撰稿人如何利用语码本身所传递的意义影响广告受众对广告人目的及意图的了解。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。