查询词典 snab

The plasmid of pMD-T-GO and pPIC3.5K were extracted from JM109 strain and then glycolate oxidase gene fragment was cloned into the expression vector (pPIC3.5K). The recombinant plasmids were transformed into E.coli TOP 10Fˊ. The recombinant clones were identified by PCR, digested with SnaB I /Not I and then sequenced.

本文首先从大肠杆菌JM109中提取质粒pMD-T-GO和pPIC3.5K，将菠菜乙醇酸氧化酶基因c DNA片段克隆至表达载体pPIC3.5K，转化进E.coli TOP 10Fˊ，利用设计的引物进行PCR扩增、SnaB I和Not I酶切鉴定，最后进行序列分析。

Or I will snab her necks.- No way, we don't give her a shit.

否则我掐死她-没门儿我们才不管她呢

PCR productsdigested by SnaB Ⅰ and Not Ⅰ were inserted into the correspondingrestriction site in the expression plasmid pPIC9K.

用限制性内切酶 SnaBⅠ和 NotⅠ酶切 NGF cDNA 的 PCR 产物，将其插入到载体 pPIC9K 中相应的限制性内切酶位点，构建表达载体pPIC9K-NGF.3。

Methods Digest recombinant plasmid pU57-SOD with SnaB Ⅰ and Not Ⅰ and insert the obtained SOD gene into expression vector pPIC9k.

方法根据酵母密码子偏好性，合成人Cu，Zn-SOD基因，克隆至真核表达载体pPIC9k中，转化毕赤酵母GS115。

Several fragments containing the replication origin were cloned into replicon probe vector pUC-MEV constructed in this study. After screening a 4. 8kb PvuⅡ-SnaBⅠ fragment were determined to be the minimum replication region of the plasmid.

将包含复制起始原点在内的数个片段分别插入构建的嗜盐古生菌的复制子探针载体pUC-MEV中，通过筛选得到了pHH205最小复制功能片段。

A SnaBⅠand NotⅠrestriction sites were introduced into NGF generespectively by PCR .After being amplified by PCR,this NGF genecontained SnaB Ⅰ and Not Ⅰ restriction sites at its 5'and 3'endsrespectively.

设计合成一对特异引物，分别带有 SnaBⅠ和 NotⅠ的限制性内切酶的识别位点，以 pPIC9K-NGF重组质粒为模板，用 PCR 方法扩增 NGF cDNA 片段。

All of the 58 restriction endonucleases were used to investigate the mtDNA diversity. Four restriction endonucleases, DraⅠ、SnaBⅠ、SspⅠ and Vsp Ⅰ, were polymorphic. Based on presence or absence of the the 215〓 SspⅠ site, all of the 13 mtDNA sequences were divided into two groups.

利用DNAClub软件对AT富集区和tRNA〓基因的DNA序列进行了模拟酶切，所有的58种内切酶中有10种内切酶在13个材料中有酶切位点，仅4种酶DraⅠ、SnaBⅠ、SspⅠ和VspⅠ表现出多态性，且主要位点差异在于SspⅠ酶切位点的有无（序列的第215位的C/T）。12个品种中有4个品种（豫早1号、豫早2号、鲁红、33）没有此酶切位点，另外8个品种有此酶切位点，野柞蚕也没有此酶切位点。

To prepare recombinant fox growth hormone, we amplified its cDNA from silver fox pituitary tissue by RT-PCR and cloned into yeast shuttle vector pPIC9K down stream of α-factor signal peptide sequence by SnaB I and Not I restriction sites. The recombinant secretion vector pPIC9K/fGH, linearized by Sal I, was transformed into histidine-deficient Pichia pastoris strain GS115 by electroporation.

为制备重组狐狸生长激素，采用RT-PCR方法，从银狐垂体中扩增fGH cDNA基因，利用SnaB I和Not I位点将fGH基因插入到酵母分泌型表达载体pPIC9K中α-因子信号肽的下游，构建成fGH基因的酵母分泌型表达载体pPIC9K/fGH，载体经Sal I酶切线性化后，通过电转移将线性化的pPIC9K/fGH转化到组氨酸缺陷型酵母宿主菌GS115中。

Methods Amplify IL-6 (23)-PE40 gene from the previously constructed recombinant plasmid pKK-IL-6(23)-PE40 by PCR, and insert to the SnaB Ⅰand NotⅠ sites of vector pPIC9. Transform the constructed recombinant plasmid pPIC0-SN to P. pastoris GS115 by electroporation, screen Mut-recombinants for expression under induction of methanol.

采用PCR技术，将目的基因IL-6(23)-PE40插入到pPIC9载体中SnaBⅠ与NotⅠ位点，电转化至巴斯德毕赤酵母GS115中，筛选Mut-型重组酵母；甲醇诱导表达，体外细胞试验检测其细胞毒性。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐