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By using electroporation transfects,the TGF〓expression vector were introduced into HL-60 cells.Thecells were allowed to grow for 1 day before beingsubjected to selection for the ability to grow in mediumcontaining 600ug/ml geneticin.Stable cell clones resistantto G418 sulfate were obtained after 2-3 WK growth withdrug-containing medium.Southern blot hybridization wascarried out.RNA slot have higher expression of TGF〓mRNA in HL-60 transfected.All results followed suggestedthat TGF〓 have suppressed the maliganant phenotype of HL-60 cells.

利用电击法将〓表达载体导入HL-60细胞中，经G418筛选培养20天后再行Southern杂交检测外源〓整合情况，利用RNA狭线杂交法证明了在转录水平上表达有所提高，进一步进行了流式细胞仪测定DNA含量，使细胞在〓期产生生长阻滞作用，细胞生长速度，在软琼脂中成集落数、在组织中的浸润性及在裸鼠中的成瘤性均发生变化，我们的实验结果表明TGF〓对HL-60细胞具有抑制恶性表型的作用。

In Year 1 (and possibly in Year 2), there is a timetabled slot each week for personal tutorials when tutors meet with their tutorial group.

与个人导师建立建设性的联系是对学生有益的，它可以使学生在需要任何建议或支持的时候，向个人导师寻求帮助。

I've compiled a list of bear tanking gear using m y current gear on the PTR for base stats, and rated several feral pieces for each slot.

我用我目前身上的装备当基本数值来编辑这张表，评估每一件野性装备。

As you get close to finishing you will be using the square needle file to file the forward and back parts to the slot.

正如你亲近整理，你将要使用的广场针档案文件的前进和回零件缝隙。

They slot over the stringers and are fully welded so that they cannot overturn or break free under load.

他们插槽的特约记者，并完全焊接，使他们不能推翻或摆脱根据负荷。

With the Taz Machine,as compared to the Slot Machine, you want to delay the next input so the kite continues to a nose-high orientation.

Taz Machine与Slot Machine比较，不同之处是你会稍稍延迟下一个动作，这样风筝就会持续这种机头抬起的转动，转过多一点的角度。

He went out and got changed, put on a pair of Speedos with his balls hanging out, some goggles, and did a 25-minute slot telling jokes.

此后他出去，做了些改变，戴上一个速率器，把球挂在外面，戴上太阳眼镜，讲了25分钟的笑话。

Narrow slot skylights produce a dramatic line of light across the water.

窄槽天窗急剧线生产的光在水中。

Due to FMI technique basing the advantages of high-resolution and slot of geological information, it can be used to recognize, differentiate and evaluate alga limestone, siltstone and limestone reservoirs.

微电阻率成像测井技术具有分辨率高，携带大量地质信息的优势，能较好地识别、区分和评价藻灰岩，粉砂岩，石灰岩储层。

Application of PLC in Isa WHB rapping system;2. Through measures taken,such as replacing cathodic wires,improving the rapping system,and installing slot type dust-collecting plate at the outlet etc.

通过更换阴极线、改进振打系统、出口增设槽形收尘板等措施，使电除尘器除尘效率和可靠性大幅度提高，烟尘排放浓度基本小于100 mg/m3，符合排放标准。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。