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The fragment size can be amplified by 295 bp through the primer, in order to prevent false positive from appearing, a pair of internal control primers C34 is designed through the invention according to a bull autosome 3 reported sequence, the fragment size is amplified by 208bp, dual PCR amplification is performed to single bull sperm through the two pairs of primers, and then the final evaluation is performed to the sperm separation purity according to the statistical analysis to the detection result.

本发明提供了一种检测X、Y精子分离纯度的引物，该引物是针对牛Y染色体上性别决定基因Sry通过PCR错配技术设计而成，通过该引物可扩增片段大小为295bp，为了防止假阳性出现，本发明根据牛3号常染色体报道序列设计了一对内标引物C34，扩增片段大小为208bp，通过上述两对引物对单个牛精子进行双重PCR扩增，然后根据对检测结果的统计分析，对分离精子纯度做出最终评价。

Photographic paper texture photos would normally have been developed, the media meet the " ouality business card printing technology " available with photos as realistic as possible, and you can provide a picture size from the General to the A3 + four size, suitable for all levels of user business card printing; kaixin-stick is a unique business card printing medium, the user you can use a business card printing papiermaschinen software provides more than 300 species of colourful background image box and photo overlappedness, the most imaginative, made of very attractive paper kaixin-pastes; ink－jetprinting matte coated paper is a three-metre-long A2 format with oil painting business card printing effect of medium, apply to the business card printing a variety of significant photos, pictures of art and design needs; users can use the t-shirt transfer paper production has its own unique style t-shirt, the effect is quite good; ink－jetprinting transparency can provide the perfect projection effect, apply to the projection, Convention and advertising and lively, images, making 36 0dpi; banner headline in the paper available 360dpi, 720dpi or 1440dpi business card printing that is tailored to a variety of activities business card printing banner.

照片纸的质地与通常冲洗的照片一样，该介质配合"照片质量制卡技术"可获得同照片一样逼真的效果，并且可以提供从普通照片大小到A3+的四种尺寸，适合各层次用户照片制卡的需要；开心妙妙贴是一种独特的制卡介质，用户可借助制卡机配套软件提供的300余种五彩缤纷的背景图框与照片叠加，进行最富想象力的搭配，制成极富个性魅力的开心妙妙贴贴纸；喷墨布纹纸是一种A2幅面三米长具有油画制卡效果的介质，适用于制卡各种大幅照片、艺术设计图片的需求；用户可以用T恤转印纸制作具有自己独特风格的T恤衫，效果相当不错；喷墨透明胶片能提供完美的投影效果，适用于投影、会议及广告等，图象生动逼真，可制卡36 0dpi；横幅大标题纸可用360dpi、720dpi或1440dpi 制卡，适合为各种活动制卡横幅。

Beijing Real Estate and Construction Bar Association Professional Committee of Liu Hua, deputy director of the Law Society: The contract agreement in the area, it seems to me there are at least two flaws: First, it agreed to construction area for the sale area, construction area is passed and the pool area and the area, and in this contract on the pool area also lacks transparency, developers will not tell you specific assessments where the location of the area, the specific area is the number, which for him provided an opportunity for fraud; Secondly, the contract agreed by the parties, permit the forecast area of 3% error, if the pool size increases as long as no more than 3%, you can only increase the pool for this increase the size back the principal.

北京地产及建造界律师协会专业委员会刘华副主任律师公会：合同协议，在该地区，在我看来至少有两个缺陷：第一，它同意建筑面积的销售区，建筑面积是通过和游泳池区和地区，并在此合同的游泳池也缺乏透明度，开发商不会告诉你具体评估那里的位置，面积，具体领域是的数目，这为他提供了一个机会，欺诈；其次，该合同约定，允许预测面积的3 ％的错误，如果池大小的增加，只要不超过3％，你只能增加池的大小增加本金。

Wister rats can be anesthesiaed by 3% Barbital- natrium (0.1ml/100g ip), then rat can be immobilitied on the operation table and makes a 2cm abdominal-median-mcision to detach abdomial aorta and uses seven-size suture silk to ligate abdomial aorta with seven-size needle, and draw out probe. At the same time rats were injected ten-myriad-unit Benzylpenicillin into abdominal cavity and sutured abdominal cavity.

取Wistar大鼠115只用3%戊巴比妥钠(按0.1ml/100g ip)麻醉，将动物固定于手术台上，腹部正中切口2cm后钝性分离腹主动脉，在肾动脉分枝上用7号缝合线将腹主动脉与7号针头一起结扎，然后抽出探针形成腹主动脉狭窄(约50%)，同时腹腔一次性注射青霉素10万单位关腹缝合。

The effects of synthesis temperature on the lattice parameter,the crystallite size and the content of CO2-3 of the prepared samples,the effects of the concentration of reactants on the particle size and morphology,and the effects of the molar ratio of barium hydroxide to tetrabutyl titanate on the molar ratio of bar...

研究了合成温度对样品的晶胞参数、晶粒大小和CO32-含量的影响；反应物浓度对样品的颗粒大小和形貌的影响；氢氧化钡与钛酸四丁酯的摩尔比对样品中钡钛摩尔比的影响。

By starting with the basic theory of metropolis ring spatial development and drawing the experience of domestic and foreign metropolis ring, the paper makes a discussion on efficiency of metropolis ring pattern, development phases and spatial structure. It points out the three main phases of metropolis ring development—rudiment, growth and autumn. From the spatial structure, the paper stresses that metropolis ring has the features of "ring and layer", the size of the "ring and layer" has the direct ratio with city size, convenient degree of external transportation and city radiant intensity. It owns the idea that adjustment and control of metropolis ring spatial development should reflect the following concepts, including globalization, regional integrated harmony, views of scientific development, harmonious society, consultative city planning, people-oriented principle, guided city planning and responded city planning.

从都市圈空间发展的基础理论入手，借鉴国内外都市圈发展的经验，本文对都市圈模式效应、发展阶段和空间结构进行了探讨，认为都市圈发展阶段主要分雏形期、成长期和成熟期三个阶段；从空间结构看，都市圈具有"圈层式"的特征，圈层的大小与城市规模、城市对外交通的便利程度、城市对外辐射强度成正比；都市圈空间发展调控应体现全球一体化理念、区域整体协调理念、科学发展观理念、构建和谐社会理念、协商规划理念、以人为本理念、导向型规划理念和规划应变性理念。

Bataan is the second largest class of ship and with just Navy personnel on board the crew size is 1,600 personnel, but when it has a complete Marine contingent on board the crew size increases to approximately 3,200 personnel.

U.S.S。 Bataan是第二大级别的船，仅船上海军乘员就有1，600名，但是，当整个海洋分遣队在船上，乘员数量将增至约3，200名人员。

At this point the image will be in your slide, but may not be of a satisfactory size; if necessary, re-size it as described in Resizing a picture .

这时图像会在您的幻灯片上，但可能不是一个理想的尺寸。如有必要，按调整图片的大小中所述调整其尺寸。

All he wants is anal sex and my asshole is now the size of a 50 cent piece when it used to be the size of a 5 cent piece.

他总是插我的屁屁，以前我的屁眼也就是五分硬币那么点，现在被他弄得有五毛的那么大了！

We used NCAR/NCEPT anew analyze data and the method of bilinearity insert to fix on profile distribution of several physical field in the air on observation point and the stream field of 700hPa. We primary discussed the possible mechanism to influence aerosol size distribution.The atmospheric condition of 1000hPa~700hPa level had great influence on aerosol size distribution, and influence on coarse particle mainly below 850hPa level. The horizontal wind velocity of 925hPa level is a primary gene which contributed to coarse particles in the air. Vorticity profile especially below 700hPa level is a key gene to influence diffuse and convergence of local aerosol. The air vertical motion velocity greatly influenced aggregation and remove of aerosol particles. The vertical distribution of air temperature mainly influenced vertical transportation of aerosol.

利用NCAR／NCEP逐日再分析资料，通过双线性插值方法，确定观测点上大气中几个物理量的廓线分布；及700hPa的流场，初步探讨了影响大气中气溶胶粒子谱分布的可能机制： 1000hPa～700hPa的大气状况对气溶胶粒子谱分布影响显著，对粗粒子气溶胶的影响显著的主要集中在850hPa以下；925hPa的水平风速是影响大气中粗粒子气溶胶的一个主要因子；涡度廓线(尤其是700hPa左右及以下的涡度廓线)是影响局地气溶胶的扩散的一个关键因子；大气的垂直运动速度对气溶胶的聚合及移出影响较大；大气温度的垂直分布主要影响气溶胶垂直输送。

I will endeavour to find you some assistance.

我尽力帮你找人帮忙。

At first I only know bruck is the idol of American younglings, afterwards I returned back to Taiwan ,even in Beijing last year ,I saw her poster everywhere, I was so surprised at her charm.

起初我只晓得布鲁克雷德丝是美国少男少女崇拜的偶像，后来回台湾，甚至去年在北京，居然也四处看见她的海报，才惊讶她的魅力之大。