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Methods The hyperpigmented intermediate-spl it-thickness skin grafts of 16 patients' face and cervicum 1 year after operation was harvested and the normal skins around the recipient site and the donor site were used as controls.

取患者自愿捐赠面颈部自体中厚皮片移植术后1 年的色素沉着皮片16 例，以自体移植皮片受区周围及原供区皮肤作为对照。

Methods:We ablated the ventricular tachycardia of 12 patients with normal hearts by the catheter, using pace mapping and activation sequence mapping. The former original target was the site where the pacing QRS was entirely consistent with IVT(Idiopathetic Ventricular Tachycardia) attack. And the latter original target was the site of in advance of earliest high frequency and low amplitude local potential on surface electrocardiogram QRS≥20ms, using activation sequence mapping. Results: Among 12 patients, the IVT originated from right ventricle in 2 patients, from left ventricle in 10 patients.

对12例正常心脏室速进行导管消融，采用起搏标测与激动顺序标测，前者以起搏时与室速QRS波型形态完全相同，后者以激动顺序标测法定位，以记录心动过速时较体表心电图QRS提前≥20ms的最早高频低振幅局部电位处为消融靶点，结果：12例患者室速起源部位分别为：右心室2例，左心室10例，疗效100%。

According to the successful experiences on transgenic animal production and animal cloning, gene targeting could be utilized in many fields including theory and application research on genomic modification (such as site-specific gene repair, genetic defect therapy, gene knockout, infaust gene modification, even the site-specific integration), improving expression level of specific gene in animal mammary gland bioreactors, and promoting the industrialization of transgenic animal production.

结合现有转基因动物和动物克隆技术方面的经验，利用基因打靶技术，开展动物基因组修饰的理论和应用研究，即可定点进行基因修复、治疗遗传缺陷、基因敲除失活不利基因，还可最终解决动物乳腺反应器存在的随机整合率高而表达率较低的问题，实现定点整合及乳腺特异表达，为转基因动物的产业化生产创造条件，代表了当今动物遗传学发展的主流方向。

The nasolabial skin flaps pedicled with the infraorbital artery are easy to be obtained, blood-supply are reliable,and the colouration and texture of donor site are similar to the recipient site.

以眶下动脉为蒂形成的鼻唇沟皮瓣，具有采集方便、血供丰富、色泽质地与受区相近等优点，适用于各种类型鼻部缺损的修复。

ConclusionThe nasolabial skin flaps pedicled with the infraorbital artery are easy to be obtained, blood-supply are reliable,and the colouration and texture of donor site are similar to the recipient site.

以眶下动脉为蒂形成的鼻唇沟皮瓣，具有采集方便、血供丰富、色泽质地与受区相近等优点，适用于各种类型鼻部缺损的修复。

The picture that use cruel dazzles: Do not pay attention to those pictures too, apparently they may attract a reader, but search engine to SE for it is infructuous only, must consider material of site content layout to wait above all so friendly to SE, easier by capture, next, in again proper most the site undertakes got-up.

使用酷炫的图片：不要太注重那些图片，表面上他们可能吸引读者，但是对SE来说只是徒劳，所以首先必须考虑站点内容布局素材等对SE友好，更容易被抓取，然后，在再适当的最站点进行修饰。

These results indicated that palmatine had one common binding site on the immobilizedβ_2-AR column,the association constant was 2.93×10~4 L/mol at pH 7.2 and 25℃,while jatrorrhizine had two bingding sites,the association constants at these two sites were 1.93×10~4 and 1.56×10~5 L/mol,respectively.And the molar ratio of weak bingding sites to strong binding sites was 93:7.Palmatine and jatrorrhizine had competed for low-affinity site in immobilizedβ_2-AR.

结果表明，盐酸巴马汀与β_2-AR只有一类结合位点，在流动相pH 7.2，温度25℃时盐酸巴马汀与β_2-AR的结合常数为2.93×10~4 L/mol；盐酸药根碱与β_2-AR有两类结合位点，在这两类结合位点上的结合常数分别为1.93×10~4和1.56x10~5L/mol，两类结合位点数的比值为93：7，且盐酸巴马汀和盐酸药根碱竞争低亲和力的结合位点。

Similar to the oxidation process of Si(111)-7×7 by water,the gradual insertion of S atom into Si_a-Si_s backbond is possible to occur,and the insertion pathway,which begins with directly destroying Si_a-Si_s backbonds by a foreign H_2S molecule,remains more favorable.However,when H_2S attacks at the Si_a site to split Si_a-Si_s backbond with its S or H atom,there is no distinct thermodynamical and kinetical difference between these two attacking behaviors,whereas Si_a-Si_s backbond is easilier broken when H_2O attacks at the Si_a site with its O atom.This mainly lies in the fact that the electronegativity of oxygen is larger than that of sulfur.

5类似于H_2O对Si(111)-7×7的氧化历程，S原子逐步插入Si_a-Si_s键是可以发生的，且H_2S直接打开Si_a-Si_s键进而使S原子插入的途径仍是动力学上较为有利的，但H_2S以S或H原子进攻Si_a位从而打开Si_a-Si_s键的两种反应机制在动力学和热力学上并没有表现出明显的差别，而当H_2O以O原子进攻Si_a位时，Si_a-Si_s键则较容易断裂，这应归因于O的电负性比S大。

Only Lamin A was observed to be able to be co-immunoprecipitated with 8B7 spectrin.Lamin A is a component in lamin and functions as a common anchoring site for many proteins.It plays important roles in maintaining normal morphology of nuclear envelope,presenting anchoring site for chromatin and reconstructing of nucleus.8B7 spectrin may function through interaction with Lamin A.

Lamin A是核纤层的组成成分，由LMNA基因编码，是核内许多蛋白分子的共同锚定物，在维持核膜的正常形态，提供染色质的锚定位点以及在核的重组过程中可能发挥重要作用。8B7血影蛋白可能经由Lamin A发挥功能。

Assisted by DNA2.0 and Gene2Oliga software, we optimized the codon usage and secondary structure of RNA and induced enzyme sites Cla I (237 site) and Pst I (475 site) into the gene. In the first step, fragments F1 (237 bp), F2 (238 bp) and F3 (422 bp) were separately synthesized by assembly PCR. In the second step, fragments F1, F2 and F3 were separately digested by Cla I and Pst I, and then ligated into a full length lipA gene.

首先在DNA2.0和Gene2Oliga软件辅助下对lipA基因密码子及RNA二级结构进行优化并引入Cla I(237位)和Pst I(475位)酶切位点；通过组装PCR分别合成lipA基因的各片段F1(237 bp)、F2(238 bp)和F3(422 bp)；通过该基因内的Cla I和Pst I限制性酶切位点连接成完整的全长lipA基因。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！