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We studied the optimizaiton, accuracy and reliability of this technique by definition the genotype of five common mutation loci of β-globin gene in the carrier with CD41-42 mutation and that of β-globin gene of normal people's single lymphocyte and single blastomere.

我们采用了一种利用单细胞标本通过二重巢式PCR并结合ARMS技术确定中国人群中广泛的β-地中海贫血突变谱的基因型的策略，通过对含有β-珠蛋白基因CD41-42突变的携带者5个该基因常见突变位点基因型的确定，以及正常人的单个淋巴细胞、单卵裂球的β-珠蛋白基因该5个位点基因型的确定，对这种技术条件的优化、准确性和可靠性进行了研究。

Methods The high frequency mutation region G380R of fibroblast growth factor receptor 3(FGFR3) gene was amplified by nested PCR with single lymphocyte and single blastomere.

采用巢式 PCR扩增单淋巴细胞及单卵裂球的成纤维细胞生长因子受体 3基因的高发突变位点 G380 R区域，用限制酶 Bfm 消化 PCR产物，10 %聚丙烯酰胺凝胶电泳检测。

Methods Multiplex nested PCR was used to amplify the sex linked amelogenin and IL-3 genes from human single lymphocytes and single blastomere.

方法取成人外周血单个淋巴细胞和单卵裂球，采用多重巢式多聚合酶链技术同时扩增性别相关基因Amelogenin基因和IL-3基因。

"I do not think any single organization or any single meeting is going to solve all these problems," said Boucher.

他说：我不认为任何一个组织或任何一次会议能够解决这些问题。

Tween flat seat bucket shop to make a single copy 4, the first meeting between the shop floor as a shop for five pairs of copy, and the 45-degree oblique China and the remaining layers make up between the Big Dipper as a single copy shop to make four, double only Chinese the third, five, seven, nine, eleven, thirteen addition to China, but also a 45-degree slope.

平座补间斗为单抄四铺作，第一层补间铺作为双抄五铺作，且为45度斜华，其余各层补间斗为单抄四铺作，双层只用华，第三、五、七、九、十一、十三层除华外，还出45度斜。

Three different types of hoses are available for use in the Abaque Series pump: Natural rubber Single-stage single suction in-line centrifugal pumps (generally resistant to diluted acids and alcohols) Buna or NBR (wear resistant to oily products) and EPDM (high chemical resistance especially with concentrated acids alcohols and ketones).

三种不同类型的软管可在Abaque系列泵使用：天然橡胶，丁腈橡胶和丁腈橡胶，三元乙丙橡胶（高耐化学性，尤其是浓酸，醇酮）。

In this paper we combining connecting tunnel project of Subway Line 4 at Xidan-lingjing bystreet in Beijing. To aim at the difficulties of building large-span connecting tunnels in the fine sand layer, through roads and underground pipelines, construction nearby buildings and other project difficulties. Using theoretical analysis, numerical simulation, field tests and other research methods to comprehensive and systemic research the stratum deformation mechanism and process control techniques of shallow excavation method, and a series of results were obtained. Firstly, use the stochastic media theory to analyze the stratum deformation mechanism, concretely analyze the analytic equation of surface horizontal displacement and deformation which were caused by the excavation of single-hole and double-hole tunnel, and further discussion on ground settlement, vertical deformation, surface horizontal displacement and horizontal deformation. Analyzing the main influence factors of surface displacement, and combined with practical work to proof the theory which was applied to shallow buried single-hole tunnel in fine sand layer was scientific.

本文结合北京地铁四号线西单～灵境胡同渡线隧道工程，针对在粉细砂地层中修建大跨渡线隧道，下穿道路和地下管线，临近建筑物施工等工程难点，利用理论分析、数值模拟、和现场监测等多种研究手段，对浅埋暗挖隧道地层变位机理及控制技术，深入地进行全面和系统的研究，并取得以下成果：(1)引入随机介质理论，对地层变位机理进行了分析，具体分析了单、双孔隧道开挖引起的地表位移及变形的解析方程，并深入探讨了地表沉降、垂直变形、地表水平位移和水平变形的特点，同时分析了影响地表位移的主要因素，并结合工程实例，佐证该理论应用于粉细砂地层浅埋暗挖单孔地铁隧道的科学性。

It is to divide the flow units, and then calculate the single well controlled reserves regarding the flow unit as the calculating unit, finally, compare and analyze the results from this method, single well controlled area method and dynamic pressure drawdown method respectively. The comparison result proves the scientificity and practicability of the flow unit method.

最后与通过划分单井控制面积法计算得到的单井控制储量及通过动态储量计算方法计算得到的单井动储量进行对比分析，其结果论证了流动单元法计算单井控制储量的科学性和实用性，使流动单元的研究在低渗透气田油藏精细描述中得到了进一步的推广和应用。

We use a bulb for heating and with the help of double-direction controllable silicon that controlled the calefaction value, and then detected by temperature sensor, the input signals are amplified and analog signals are transformed to digital signals. Finally the signals reach single-chip. Then single-chip compare the value of preliminary temperature with ones of pragmatic temperature, and modulate to operate.

主要采用的是光源加热，由双向可控硅控制其加热量的大小，由温度传感器检测现场温度，该信号经过放大及A/D转换后进入单片机，单片机将设定的温度值与实际检测到的值进行比较运算，并输出。

The intralellular labeling methods developed for single cell analysis are summarized in this review, including cell itself acting as a microreaction chamber for derivatization, dericatization mediated by liposome or polyethylene glycol to increase permeabilization of cell membrane, on- and post-column derivatization during single cell analysis with capollary electrophoresis or microchip electrophoresis and labelling of cells with quantum dots.

本文综述了在单细胞分析中常用的荧光标记方法，包括细胞作为微反应器的衍生法，借助于脂质体与聚乙二醇等增加细胞膜通透性的衍生方法和在毛细管/芯片毛细管电泳分析单细胞时柱上衍生和柱后衍生法以及量子点的标记法等。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。