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The method involves exposing the cells to a compound having the formula I in which: w is a nucleic acid x is a non-amino acid or non-peptide nucleic acid binding group y is a spacer having a chain length equivalent to 1-30 carbon-carbon single covalent bonds or is absent R4 is H or halogen or CH2O-R3; and R1, R2 and R3 are the same or different and are either hydrogen, methyl, ethyl, alkyl, alkenyl, hydroxylated alkyl, hydroxylated alkenyl groups or ether containing alkyl, alkenyl, hydroxylated alkyl or hydroxylated alkenyl groups optionally being an acyl group having a carbon chain length equivalent to 3-24 carbon atoms saturated or unsaturated, with the proviso that at least one of R1, R2 or R3 includes a group having a carbon chain of 3-24 carbon atoms saturated or unsaturated, or to a compound having the formula II in which: w is a nucleic acid x is a non-amino acid or non-peptide nucleic acid binding group y is a space having a chain length equivalent to 1-30 carbon-carbon single covalent bonds or is absent, R5 is alkyl, alkenyl, hydroxylated alkyl, hydroxylated alkenyl group or ether containing alkyl, alkenyl, hydroxylated alkyl or hydroxylated alkenyl group optionally being an acyl group having a carbon chain length equivalent to 3-24 carbon atoms saturated or unsaturated, with the proviso that R5 includes a group having a carbon chain of 3-24 carbon atoms saturated or unsaturated.

该方法包括使细胞暴露于具有式的化合物，在式中：w是核酸，x是非氨基酸或者非肽核酸结合基团，y是具有等于1―30个碳―碳单共价键的链长的间隔基或者不存在，R4是H或者卤素或者CH2O－R3；R1、R2和R3是相同的或者不同的并且是氢、甲基、乙基、烷基、链烯基、羟基化烷基、羟基化链烯基或者包含烷基、链烯基、羟基化烷基或羟基化链烯基的醚，任选地是来源于具有等于3―24个碳原子的碳链长的饱和或不饱和脂肪酸的酰基，其条件是R1、R2或者R3的至少一个包括具有3―24个碳原子的饱和或不饱和碳链的基团，或者使细胞暴露于具有式的化合物，在式中：w是核酸，x是非氨基酸或者非肽核酸结合基团，y是具有等于1―30个碳―碳单共价键的链长的间隔基或者不存在，R5是烷基、链烯基、羟基化烷基、羟基化链烯基或者包含烷基、链烯基、羟基化烷基或羟基化链烯基的醚，任选地是具有等于3―24个碳原子的饱和或不饱和碳链的链长的酰基，其条件是R5包括具有3―24个碳原子的饱和或不饱和碳链的基团。

The research on the conditions of chlamydospore production for the liquid fermentation showed that, the better single factors were oat liquid media, 30℃, 120r/min surge culture, sucrose as carbon source,(NH_4)_2SO_4 as nitrogen source and the ratio of 20 to 1 for C/N. Under the better single factors mentioned above, the largest quantity of chlamydospore, i.e.3.05×10~7spores/ml, was obtained on the 10th day after culture.

木霉T-33菌株产生厚垣孢子的发酵条件研究表明，采用燕麦粉培养液、30℃、120r/min振荡培养、250ml三角瓶装入80ml培养液、接种5片活化2d的5mm直径的菌片，碳氮源选择蔗糖和硫酸铵，C/N为20/l，足适合T-33菌株产孢的最佳单因素条件(碳氮源实验基于Czapek培养基)；用这些最佳单因素组合(不包括碳氮源和C/N结果)培养木霉T-33绘制了产孢曲线，发现在培养第10d得到最大的厚垣孢子产量3.05×10~7个/ml。

First, a chlorinator was self-designed in this paper, and dichloride of 2-methyl-1-propene was synthesized by 2-methyl-1-propene reacted with chlorine fast in this reactor. By means of the Orthogonal Experiments design and single factor experiments, the influence of several reaction variables on the yield were examined and the optimum reaction conditions under the intervallic operation were obtained, the yield and selectivity of dichloride of 2-methyl-1-prop-ene were more than 65% and 80% respectively. Second, the preparation of 2-methylene-1, 3-propanediol, which dichloride of 2-methyl-1-propene reacted with alkali was investigated. By means of the Orthogonal Experiments design and single factor experiments, the influence of several reaction variables on the yield were examined and the optimum reaction conditions under the intervallic operation were obtained, the conversion of dichloride of 2-methyl-1-propene was 100%. Moreover the preparation of MPO, which 2-methylene-1, 3-propanediol reacted with hydrogen, in presence of Al_2O_3-Pd catalyst, was gotten, and the product was appraised.

本文在氯化反应过程中，自行设计制作出了氯化管式反应器，在该反应器中异丁烯和氯气迅速混合反应生成二氯异丁烯，根据该氯化反应特征利用正交试验和单因素试验考察了有关因素的影响并优化得到了最佳工艺条件，二氯异丁烯的总收率达65％以上，选择性达80％；接着，对二氯异丁烯进行水解反应制得了2-甲烯-1，3-丙二醇，讨论了相关因素对水解反应的影响，并通过正交试验和单因素试验得到了较佳工艺条件，二氯异丁烯的转化率达100％；同时还对制备的2-甲烯-1，3-丙二醇在常压下采用三氧化二铝负载钯催化剂催化加氢得目的产物MPO，并对产品进行了鉴定，从而打通了整个工艺路线。

The theory of this kind of sensor is introduced. The structure and design of the measure instrument on-line based on the single chip processor is discussed.The symbol adjust circuit of the sensor and the 8031 single chip microcomputer system are designed and the main program flow chart is described.

介绍了这种传感器的工作原理，讨论了基于8031单片机的微量水分在线测定仪的结构，设计了传感器信号调理电路及8031单片机系统，给出了软件流程图。

This thesis circumambience a hot topic in "single slice machine control the GSM mold piece receive and dispatch a message", elaborated a homologous GSM wireless terminal of design project, development method and development process, made use of 8 bit AT89S51s of Atmel company a single slice machine and Siemens company most the new generation GSM mold piece TC35i to carry out a GSM Mode which has a message to receive and dispatch function in the design is two prototypes.

本论文围绕"单片机控制GSM模块收发短信"这一热点课题，阐述了相应的GSM无线终端的设计方案、开发方法和开发过程，在设计中利用Atmel公司的8位AT89S51单片机和Siemens公司的最新一代GSM模块TC35i实现了一款具有短信收发功能的GSM Mode二原型。

Examples of basic units might be a single class or a single function.

基本单元的例子可能是单个类或单个函数。

This article detailedly expatiated upon the principles, contents and methods of cropland classification; it analyzed the necessity to combine the work of cropland classification with GIS technology, summarized the attributions of ARC/INFOand some other related softwares; it established the technologic route of cropland classification supported by GIS, by a case study in Liwu Town,Li County Hebei Province, this article expatiated the practicable mechanism of combining the function of ARC/INFO with the work of cropland classification, and put forward some new ideas and approaches of utilizing GIS: this paper put forward the idea of coding the polygons in the basic graphic database such as soil map and land use map according to their attributes, and put forward the detailed coding project; based on the code databases, it expatiated upon the detailed approaches to create single梖actor map and output the final classification map; it put forward the principles and the method of creating classification units in the environment of GIS, that is how to keep the lines of soil polygon, the border of districts and the important objects such as residential areas, banks of rivers and roads ,and how to overlap single梖actor soil map and land use map, and to delete some vector lines of the overlapped map appropriately; it detailed the methods of creating mathematic model of classification coefficients in the environment of computer, and used FOXPRO6.0 programs to realize the automation of calculating coefficients; it represented the method of plotting the final map by overlapping various coverages according to the code tables and lookup tables, the process of outputting the cropland classifying map was finished by program.

本文法对耕地等别划分的原则、内容及方法作了较详细的阐述，介绍了多系数综合修正法的耕地等别计算方法及其具体应用和AHP的确权方法；分析了耕地等别划分工作与地理信息系统技术相结合的必要性，简要介绍了地理信息系统软件ARC／INFO及其他相关软件的功能特点，制定了地理信息系统支持下耕地等别划分的技术路线，通过对河北省蠡县蠡吾镇的实证研究，重点阐述了ARC／INFO及相关数据库软件与耕地等别划分结合的有效机制及GIS应用的一些新思想、新方法：提山了对土壤图和土地利用现状图等基础图形数据库进行特征编码的思想和具体的编码方案，阐述了基于与图形库相链接的特征编码表，单因素图提取、成果图的输出的具体实现方法；提出了GIS环境下耕地单元划分的原则和方法，即在保留土壤因素图斑界线、行政界线和重要地物等的原则下，将土壤单因素图和土地利用现状图进行拓扑叠加，对叠加图中的矢量线进行适当的删除；阐述了在计算机环境下，耕地分等参数计算模型建立的方法，并用FOXPRO6.0语言程序实现了参数计算的自动化；阐述了根据图形库特征编码表和建立绘图符号查找表，按不同图层叠加绘制成果图的方法，该过程用命令程序实现耕地等别图的输出。

A collating element is any single character, or any sequence of characters that collates as a single unit.

对照元素是任意的单个字符，或对应於某个单个单元的字符序列。

An optimization model for combinatorial double auction based on integer programming method is established and transformed into single-sided auction,which will benefit to solve combinatorial double auction with the method used for single-sided auction and to make the problem much more easier.

本文运用整数规划的方法建立了一个组合双向拍卖问题的一般化模型，并通过逐步转化，把组合双向拍卖简化为单边拍卖，有助于利用求解单边拍卖的方法来求解复杂的双向拍卖问题，使这一问题的研究得到了简化。

Appling gas chromatography method to determine single layer's output and water cut of commingle producing well enriches commingle production technology. This application not only can be used to determine single layer's output, but also water cut. This technology must play important role to develop Putaohua and Fuyu reservoir effectively.

气相色谱技术用来确定合采井分层产量、含水的应用技术丰富了葡扶合采技术，其应用不仅可以确定合采井分层产量，而且可以定性确定两套油层的含水，对研究两套油层的动用状况必将发挥重要作用。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。