查询词典 single step method

Based on the original research of the high order single step method ,this paper makes the research on the applicability of this method for the analytic expression of stiffness.

本文在原有高阶单步法研究的基础上对刚度为时间解析函数时方法的适用性进行了研究。

Methods A total RNA from ten cases of ovarian cancer and contral normal ovarian tissues were extracted by a single step method. The cDNA retro-transcribed from equal quantity mRNA derived from ten cases of ovarian carcinoma and normal ovarian tissues, which were labeled cDNA strand with Cy5 and Cy3 fluorescence as probes.

按一步法分别抽提10例人卵巢癌组织和对照正常卵巢组织的总RNA并纯化mRNA；分别从10例取等量的人卵巢癌组织和对照组织的mRNA分别混合后逆转录合成以Cy5和Cy3标记的cDNA一链做探针，混合后在含有8464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

Methods］ A total RNA from 10 cases withovarian papillary serous cystadenocarcinoma and normal ovarian tissues were extracted by a single step method of gene chip. The cDNA retro?鄄transcribed from equal quantity mRNA derived from 10 cases with ovarian papillary serous cystadenocarcinoma and normal ovarian tissues. The cDNA strand was labeled with fluorescent Cy5 and Cy3(normal ovarian epithelial cells) as probes.

方法］ 采用基因芯片技术，按一步法分别抽提10例卵巢浆液性乳头状囊腺癌患者的癌组织和正常卵巢组织的总RNA并纯化mRNA；逆转录合成以Cy5荧光标记卵巢癌细胞和Cy3荧光标记正常卵巢上皮细胞的cDNA作为探针，在含有8 464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

Euler method, single step method and one-leg multistep method are considered as maily numerical computation schemes.

本文分别采用显式Euler法，单步θ-方法和one-leg方法作为数值计算格式。

Successfully applying to nonlinear structure analysis and active control with time-delay consideration,the high order single step method shows its good stability,high precision and high speed computation,etc.

高阶单步法已成功地应用于结构非线性分析及考虑时滞的主动控制等，显示了它的稳定、精度高和计算迅速等特点。

Because the successful application to nonlinear analysis of structure vibration and active control takes into account the time-delay,the high order single step method shows good stability, high precision and high computing speed, etc.

由于高阶单步法已成功地应用于结构非线性分析及考虑时滞的主动控制等，显示了它的稳定、精度高和计算迅速等特点。

Single- step method of RNA isolation by acid guanidinium thiocyanate－phenol－chloroform extraction.

重组人骨形成蛋白2成熟肽在大肠杆菌中的表达及其诱导成骨活性。

Our technology develops a single step method of producing biodiesel from the waste plant and animal oil by using DYD catalyst to carry out transesterification and esterification simultaneously.

本技术可使用不同酸价的油脂为原料，在常压下反应，反应转化率可达96%，油脂利用率100%，所获得的生物柴油产品的品质达到美国ASTM 6751标准。

All single step method and CTAB method did not work well when banana total RNA was isolated, unless modified single step method was used, in which, polysaccharide was removed by aether extraction prior to RNA precipitation.

对于富含多糖的香蕉果肉，一步法和CTAB异硫氰酸胍法的效果均不理想；改良后的一步法中增加了乙醚去除多糖并获得了较好的效果。

By exploring the filming processes of the PEDT negative pole, this paper, creatively using the vacuum single step method, has found a comparatively suitable processing condition.

通过对PEDT阴极被膜工艺过程的研究，找到了较适合的单体M和氧化剂C配比，清洗液的配方和清洗时间，烘干温度和时间，被膜和补形成次数等工艺参数。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失