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The numerical modeling process for SVE remediation is achieved after a steady alternant direction implicit finite difference format is developed. In this paper, as an example, the 60 days remediation under 4 different conditions is simulated, which proves that the clear-up result is mainly governed by soil permeability.

再采用一个稳定的交替方向有限差分解出传质微分方程，从而实现多维情况下竖井SVE修复的完整模拟过程。4组不同条件下为期60d的SVE修复过程的计算机模拟显示，土壤通透系数是决定修复效果的关键因素。

By using RegCM, the summer climatologic characters over China during1991～2005 are simulated.

并利用区域气候模式(BCCRegCM 1.0，以下简称RegCM)模拟了1991～2005年中国夏季气候的异常变化，分析了区域模式对江淮梅雨的模拟能力。

AIM: To investigate the expression of integrin subunits in osteoblasts during simulated weightlessness induced by clinostat.

目的：观察回转器模拟失重对成骨细胞整合素亚单位表达的影响。

Three experimental groups of the cultured rat osteosarcoma cells underwent simulated weightlessness in rotating clinostat for 24, 48 and 72 h respectively.

目的 通过观察回转条件下培养的大鼠骨肉瘤细胞内Ⅰ型胶原的基因和蛋白表达的变化，探讨模拟失重对成骨细胞增殖功能的影响。

The proliferation of HFE-145 cell is inhibited during simulated weightlessness on a clinostat for 12 h.

回转器模拟失重使胃粘膜上皮HFE-145细胞在12 h时出现了细胞周期阻滞和增殖抑制。

The proliferation of rat osteosarcoma cells was retarded by 72 h rotating clinostat simulated weightlessness.

回转器模拟失重72 h使大鼠骨肉瘤细胞的增殖功能降低。

METHODS: Calvarial osteoblasts of neonate rats were cultured under conditions of normal gravity(1G) and simulated weightlessness induced by clinostat respectively. The total RNA in cells was isolated in different time points (24, 48, 72 h). Reverse transcription PCR analysis was made to examine the gene expression of α5,αv and β1 integrin subunits.

培养的大鼠乳鼠颅骨成骨随机分为对照组和失重组，在实验的24， 48和72 h提取细胞总RNA，进行反转录PCR，检测整合素的α5，αv和β1亚单位mRNA表达，并计算与内参照β肌动蛋白 beta？？？？

METHODS: Calvarial osteoblasts of neonate rats were cultured under conditions of normal gravity(1G) and simulated weightlessness induced by clinostat respectively. The total RNA in cells was isolated in different time points (24, 48, 72 h). Reverse transcription PCR analysis was made to examine the gene expression of α5,αv and β1 integrin subunits. Each value was normalized against that of β actin mRNA.

培养的大鼠乳鼠颅骨成骨随机分为对照组和失重组，在实验的24， 48和72 h提取细胞总RNA，进行反转录PCR，检测整合素的α5，αv和β1亚单位mRNA表达，并计算与内参照β肌动蛋白 mRNA水平的比值。

Constructed cell model under simulated weightlessness by clinostat and confirmed the credibility by assessing the expression of biomarkers—alkaline phosphatase and osteocalcin.

对成骨活性标志物碱性磷酸酶、骨钙素和体外钙化能力进行检测以确定模型的成立。

In present study, we discussed the effects of cell microfilament on the activity of collagen type I alpha 1 chain gene (COL1A1) promoter under microgravity simulated by clinostat and/or cytochalasin B as microfilament depolymerizer in the established EGFP-ROS cell line using the method of fluorescence semi-quantitative analysis and the fluorescent stain of microfilament.

但微重力对相关基因调控的影响知之甚少，本研究以构建的基因工程细胞株为对象，以回转器模拟微重力效应，利用增强型绿色荧光蛋白（enhanced green fluorescence protein，EGFP）荧光半定量和细胞微丝荧光染色分析技术，探讨回转模拟微重力条件下，细胞微丝系统对I型胶原α1链基因（collagen type I alpha chain 1，COL1A1）启动子活性的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。