查询词典 simulated

Three experimental groups of the cultured rat osteosarcoma cells underwent simulated weightlessness in rotating clinostat for 24, 48 and 72 h respectively.

目的 通过观察回转条件下培养的大鼠骨肉瘤细胞内Ⅰ型胶原的基因和蛋白表达的变化，探讨模拟失重对成骨细胞增殖功能的影响。

The proliferation of HFE-145 cell is inhibited during simulated weightlessness on a clinostat for 12 h.

回转器模拟失重使胃粘膜上皮HFE-145细胞在12 h时出现了细胞周期阻滞和增殖抑制。

The proliferation of rat osteosarcoma cells was retarded by 72 h rotating clinostat simulated weightlessness.

回转器模拟失重72 h使大鼠骨肉瘤细胞的增殖功能降低。

METHODS: Calvarial osteoblasts of neonate rats were cultured under conditions of normal gravity(1G) and simulated weightlessness induced by clinostat respectively. The total RNA in cells was isolated in different time points (24, 48, 72 h). Reverse transcription PCR analysis was made to examine the gene expression of α5,αv and β1 integrin subunits.

培养的大鼠乳鼠颅骨成骨随机分为对照组和失重组，在实验的24， 48和72 h提取细胞总RNA，进行反转录PCR，检测整合素的α5，αv和β1亚单位mRNA表达，并计算与内参照β肌动蛋白 beta？？？？

METHODS: Calvarial osteoblasts of neonate rats were cultured under conditions of normal gravity(1G) and simulated weightlessness induced by clinostat respectively. The total RNA in cells was isolated in different time points (24, 48, 72 h). Reverse transcription PCR analysis was made to examine the gene expression of α5,αv and β1 integrin subunits. Each value was normalized against that of β actin mRNA.

培养的大鼠乳鼠颅骨成骨随机分为对照组和失重组，在实验的24， 48和72 h提取细胞总RNA，进行反转录PCR，检测整合素的α5，αv和β1亚单位mRNA表达，并计算与内参照β肌动蛋白 mRNA水平的比值。

Constructed cell model under simulated weightlessness by clinostat and confirmed the credibility by assessing the expression of biomarkers—alkaline phosphatase and osteocalcin.

对成骨活性标志物碱性磷酸酶、骨钙素和体外钙化能力进行检测以确定模型的成立。

In present study, we discussed the effects of cell microfilament on the activity of collagen type I alpha 1 chain gene (COL1A1) promoter under microgravity simulated by clinostat and/or cytochalasin B as microfilament depolymerizer in the established EGFP-ROS cell line using the method of fluorescence semi-quantitative analysis and the fluorescent stain of microfilament.

但微重力对相关基因调控的影响知之甚少，本研究以构建的基因工程细胞株为对象，以回转器模拟微重力效应，利用增强型绿色荧光蛋白（enhanced green fluorescence protein，EGFP）荧光半定量和细胞微丝荧光染色分析技术，探讨回转模拟微重力条件下，细胞微丝系统对I型胶原α1链基因（collagen type I alpha chain 1，COL1A1）启动子活性的影响。

In present study, we discussed the effects of cell microfilament on the activity of collagen type I alpha 1 chain gene ( COL1A1) promoter under microgravity simulated by clinostat and/or cytochalasin B as microfilament depolymerizer in the established EGFP-ROS cell line using the method of fluorescence semi-quantitative analysis and the fluorescent stain of microfilament.

本研究以构建的基因工程细胞株为对象，以回转器模拟微重力效应，利用增强型绿色荧光蛋白(enhanced green fluorescence protein，EGFP)荧光半定量和细胞微丝荧光染色分析技术，探讨回转模拟微重力条件下，细胞微丝系统对I型胶原a1链基因(collagen type I alpha chain 1 gene， COL1A1)启动子活性的影响。

And the model of clutter based on this is simulated.

并以此为依据进行杂波模型的仿真。

By injecting different amounts and speed of blood coagulum, acute cot pulmonale and sudden death..type of PE could be simulated.

不同时间的血液凝块作为栓子对肺血管血流量影响不同，表明血栓的性状与PE的发生有关。

However it can often be frust ating for a child when people are unable to pronounce their name.

但它往往可以令人沮丧的一个孩子当人们无法断言他们的名字。

All these compounds were obtained from this genus for the first time. Compound, 4-cumylphenol, was a new nature product.

所有化合物均为首次从本属海藻中分离得到，其中4cumylphenol为新天然产物。