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Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.
结果(1)成功构建人大细胞肺癌高低转移株差异表达基因正向消减cDNA文库和反向消减cDNA文库;(2)经蓝白菌落筛选和斑点杂交,正向消减文库获得307个阳性克隆,反向消减文库获得78个阳性克隆;(3)正向消减文库挑选55个克隆成功测序,经同源性分析最终确定差异表达基因片段23个与已知人类全长基因具有很高相似性(95%~100%),这些基因包括NME2、NPM1、MT2A、HSPE1、TAFlA、EPRS、PX19和EIF3S9等;(4)反向消减文库挑选31个克隆成功测序,经同源性检索和比对最终确定差异表达基因片段16个,其中15个与人类全长基因具有很高相似性(95%~100%),包括ANXA2、TUBB、PKN2、GNAS、EEF1A1、SSR2和RPLPO等基因。1个片段和己知人类基因仅有部分相似性,表明它可能是未被克隆的人类基因EST片段。
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There is only tangential stress and radial stress on the wellbore wall. The decrease of the temperature is similar to increase of the mud density. It makes the tangential stress reduction. The increase of the temperature is similar to the decrease of the mud density.
文中对钻井过程中由泥浆周期性载荷作用下的井壁力学响应进行了研究,得出了不同载荷作用下,恒孔隙压力井壁和稳态孔隙压力分布井壁的应力和位移解析解。
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Based on trigram models, this paper proposes a three-step method of "word—similar word—part of speech" by incorporating the similar words and solves the problem of sparse data to a large extent.
本文利用三元模型,通过引入相似词,采取"词形-相似词-词性"三步回退的策略,比较好地缓解了数据稀疏问题。
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The two volcanic materials are similar but are also different ①the partition mode of rare-earth element is similar to that of the basalt.②the increase content of the rare-earth element content in transitional layer shows that it enrichs in this segment due to the volcano events. Moreover, the comparability of the partition mode shows that the volcano-genic materials and the basalt are of the same source.③low Eu anomaly different from the high Eu anomaly of medium-acidic tuff in Meishan section, that show that their formation is different④there are plentiful basalt detritus.⑤there are enrichment of the Ti element.⑥the lower content of siderophile element of Ni and Co, especially in the transitional layer, show that there were not extraterrestrial events and big scale basic or ultrabasic volcano eruption events in this area. These characteristics show that the sedimentary source of Xuanwei Formation and Kayitou Formation is basalt.
1稀土元素配分模式与玄武岩类似;(2)稀土元素含量在异常层的增加反映了火山事件导致了它在该段地层中的富集,而配分模式的相似性反映了火山物质来源与玄武岩具有同源性;(3)低"Eu"异常区别于煤山剖面的中酸性凝灰岩高"Eu"异常模式,说明界线粘土岩成因也不同于煤山剖面的界线粘土;(4)大量的玄武岩岩屑;(5)Ti元素的富集;(6)亲铁元素Ni和Co含量较低,特别是在界线异常层含量降低,表明本区在界线处可能并不存在地外事件和大规模基性、超基性火山喷发事件,这些特征反映本区宣威组和卡以头组物质来源主要为玄武岩。
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Thiocarbonyl compounds have similar chemical properties to those of carbonyl compounds because of their similar valence electron structures.
硫代羰基化合物与羰基化合物具有相似的价电子结构,因而具有类似的化学性质。
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Their atoms have similar configurations and similar physical and chemical behaviour; the most common valence s are 3 and 4
它们的原子都有相似的配置,以及相似的物理和化学行为,最常见的原子价是3和4。
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This virus is spread similar to the hepatitis A virus and causes acute disease similar to the others.
这种病毒的传播类似于甲型肝炎病毒,引起急性肝炎也与其他病毒引起的相似。
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Stem and pedicels glabrous; leaves similar to or slightly different from each other; flowers actinomorphic; nectaries similar.
茎和花梗无毛;叶类似于不同于彼此的或稍;花辐射对称;蜜腺相似。
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The comparison of sequences showed that the determined sequence was most similar to those in GenBank (AF016546)(similarity level, 97.9%), Phylogenetic analysis indicated that the resulting sequence was more similar to those of Mycoplasma spp.(homo!ogy level, about 70%), but was farther from Anaplasma spp.( homology level, about 50%).
系统发育分析表明,所测序列与支原体属病原代表种的序列接近,同源性约为70%,而与无浆体科病原代表种的序列相差较远,同源性约为50%。
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In addition, the features of elemental geochemistry of all samples suggest that (1) the EarlyTriassic andesitic rocks, characterized by characteristic natures of island arc volcanics, likely erupted in the subduction setting; that (2) the generation of LateTriassic rhyolites with an affinity of Alike granite and synchronously main body granites in the Lincang batholith displaying similar geochemical features, is closely related with the postcollision or latecollision setting; that (3) although the LateTriassic basic rocks partly show island arc volcanics such as highAl contents and NbTa depletion, some of them possessing highMg contents and mostly having traceelement ratios similar to intraplate basalt, which indicates that they erupted in the postcollision rift environment.
元素地球化学特征表明,早三叠世火山岩具有典型的岛弧火山岩的特征,暗示其很可能形成于俯冲的构造环境;中三叠世晚期的流纹岩与临沧花岗岩基主体具有非常相似的地球化学特征,结合其流纹岩具有A型花岗岩属性,表明该区中三叠世火成岩形成于碰撞后的构造背景;而晚三叠世基性火山岩虽然在地球化学特征上显示出与岛弧火山岩相似的高铝和NbTa的亏损,但其中部分火山岩具有高镁和板内玄武岩的微量元素比值特征,说明这些火山岩可能形成于碰撞后裂谷环境。
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- 推荐网络例句
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The production moved off-Broadway to New York City, where she won the Joseph E. Calloway award for her performance, and was profiled in American Theater Magazine as one of ten young theater artists to watch.
生产提出过百老汇的新的纽约市,在那里她赢得了约瑟夫calloway奖,她的表现,并于异型在美国的战区杂志之一, 10战区的青年艺术家观赏。
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It's a risk tradeoff, and I believe that adding test support code usually wins.
这是一个风险的权衡,我相信添加测试代码通常会占上风。
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Besides, Fantine was only a moderately good workwoman
芳汀究竟还只是一个手艺平凡的工人。