查询词典 silver protein

We studied the effects of protein content, pH and ionic strength on viscosity of silver carp myofibril protein by orthogonal rotational regression design, and established the regression equation of silver carp myofibrillar protein solution viscosity with protein content, pH and ionic strengt

利用二次回归正交旋转试验设计，研究了蛋白质浓度、pH、离子强度对鲢鱼肌原纤维蛋白粘度的影响，建立相应的回归方程，分析比较了它们间的相互作用关系，确定了取得最优粘度时，各因素的组

The protein spots were visualized by silver-based staining technique with the protein silver stain kit.

用银染试剂盒进行硝酸银染色使凝胶中的蛋白质点可视化。

The gels were stained with silver, and the differential protein expressions were analyzed with Melanine 3 software. Results The protein spots of the two cell lines were 1285±51 and 1184±47 (SW480) respectively, containing 96±7 differential protein spots with 50±6 expressed or obviously increased only in LST cell line and 47±5 in SW480 cell line.

结果 LST和SW480细胞株双向电泳图谱蛋白质点数分别为1285±51和1184±47，共获得96±7的蛋白质差异点，其中50±6个点仅在LST细胞株中表达或表达明显增强，47±5个点仅在SW80细胞中表达或表达明显增强。

Changes of silver carp muscle protein s during chilled storage;2. The changes of rheology,texture and color of minced pork after the cooking respectively in water bath,retort and microwave were tested,based on which the effects of muscle protein and transglutaminase contents on the gel properties of minced pork were then investigated.

结果表明：3种热处理方法下，一定量的转谷氨酰胺酶及肌肉蛋白使猪肉糜表现出不同的颜色和质构特性；与其他两种方法相比，水浴加热使猪肉糜更具有弹性；加入MTGase后，样品的储能模量G′增大，表明MTGase的加入影响了猪肉糜的流变特性、颜色以及质构，其效力取决于猪肉糜的热处理方法。

SERS labelled immunogold was then used as probe to detect the interaction between the Goat anti-rat IgG and silver substrate (which we will use it as the substrate for solid antibody). It is found from experiment that SERS labelled immunogold can be non-specifically adsorbed onto silver surface uniformly because of the strong interaction between the protein molecule and silver.

由于我们设想以银片为免疫检测中固相抗体的基底，所以论文中首先以SERS标记的免疫金溶胶为探针，讨论了抗体与银表面间的作用，实验中发现，SERS标记的免疫金溶胶可以通过抗体与银之间的作用较好的自组装于银表面，同时，银表面上可以测到很强的苯硫酚的SERS信号。

Purified protein samples were separated by 2-DE with 17 cm IPG strips, and the protein spots were detected by fast silver staining. Six protein spots were randomly excised from the silver stained gel for primary analysis by MALDI-TOF MS. Four proteins were identified, and they were 1-aminocyclopropane-1-carboxylate synthase 2, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, F11O4.2 and Rubisco small subunit.

根据预实验结果，选用17 cm IPG预制干胶条对纯化的蛋白样品进行2-DE实验，采用快速银染法进行染色，随机挖取银染胶上6个蛋白点进行MALDI-TOF MS分析和数据库检索，鉴定了3个蛋白，它们是：1-氨基环丙烷-1羧酸合酶2，核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基和F11O4.2。

Mainly using protein - film voltammetric technique, we have achieved direct electrochemical response of lots of proteins such as hemoglobin, myoglobin, cytocrome c, cytocrome b5, horseradish peroxiase, GOD ,Vitreoscillia sp. haemoglobin, etc., at a series of protein membrane modified electrodes, for instance, protein/kieselgubr, protein/polyethylene glycol, protein/polyethyleneimine, protein/phosphatidylcholine, protein/DNA, protein/montmorillonite/polyvinyl alcohol, protein/silver nanoparticle/ cysteamine membrane modified electrode, etc., which are prepared by casting, incorperating or layer by layer self-assembly methods. Electrochemical behavior of these proteins in the films have been studied, and their structural and conformational changes have also been examined with spectrometric techniques.

三年来，在基金委的支持下，我们以蛋白膜伏安法这一新技术作为主要的研究手段，并结合其他生物、化学技术，研究了血红蛋白、肌红蛋白、细胞色素C、细胞色素b5、辣根过氧化物酶、葡萄糖氧化酶以及好氧革兰式阴性菌透明颤菌中的血红素类蛋白等多种蛋白质在以涂布和自组装方法制成的蛋白质/硅藻土膜、蛋白质/聚乙二醇膜、蛋白质/卵磷脂膜、蛋白质/聚乙烯亚胺膜、蛋白质/蒙托石/聚乙烯醇自组装膜以及蛋白质/银胶/半胱胺自组装膜等修饰电极上的直接电化学行为，结合各种光谱技术，对蛋白质在膜中的构象进行表征，探索其电子传递机理。

Results Well-resolved,reproducible 2-DE patterns of SGC7901/VCR and SGC7901 were established.Silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.Gels stained with colloidal Coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.

结果 获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。

objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

低甲氧基果胶的胶凝机理及防止预凝胶。。。他扎罗汀凝胶与克林霉素凝胶治疗痤疮。。。注射隆乳后经乳晕切口取出聚丙烯酰胺。。。目的分析利用蛋白质组学方法研究胃癌耐药相关蛋白质中双向电泳凝胶的染色显示。方法培养胃癌细胞sgc7901和长春新碱诱导的耐药胃癌细胞sgc7901/vcr，用双向凝胶电泳技术分离总蛋白，银染及胶体考马斯亮蓝染色，image scanner扫描仪扫描凝胶。结果获得了背景清晰、重复性好的双向凝胶电泳图谱，两种染色凝胶相比，硝酸银染色在样品少时显示更佳，过量则影响图像质量，而胶体考马斯亮蓝染色在上样量增加时的凝胶蛋白质点数目及丰度均增加，并无明显拖尾。结论两种显色方法受样品量影响较大，恰当选用有利于通过蛋白质组学研究胃癌耐药机制工作的进一步开展。

It was established that, the fish skin was stirred two times with 2.5% or 5% NaCl solution at a ratio of material to solvent of 1:10 at about 5℃ for 12h, about 5.9%, 6.7%, 9.1% soluble non-collagenous protein could been separated from skin of silver carp, bighead carp and grass carp, then by homogenizing with acid at a ratio of material to solvent of 1:30, about 78.9%, 84.1%, 82.0% of collagen from skin of silver carp, grass carp and bighead carp could be extracted by acetic acid or citric acid.

结果表明，鲢、鱅鱼皮经2.5％NaCl溶液(1：10)，草鱼鱼皮经5％NaCl溶液(1：10)，温度5℃左右，12h磁力搅拌处理二次，除去的可溶性蛋白分别占原总蛋白的5.9％、6.7％、9.1％。鱼皮再经加酸(1：30)溶胀、均质处理后，即可得到鱼皮胶原蛋白的粗提取液，鲢、草鱼鱼皮的酸提取回收率以醋酸为最高，分别为78.9％、84.1％，鱅鱼皮以柠檬酸为最高，约为82.0％。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。