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The antibacterial activities of most of fractions were similar to the pomegranate peel extract.(7) Respiratory inhibition experiment showed that the respiration of thallus was inhibited mainly through the hexose monophosphate pathway, which leaded to be short of NADPH, and induced metabolic function dysfunction. Growth curve assay showed that the pomegranate peel extract inhibited cytodieresis in logarithmic growth phase. After exposure to the pomegranate peel extract, bacterial protein was analyzed by SDS-PAGE. The results showed that protein synthesis was inhibited, especially for large molecular weight protein.

7呼吸抑制试验结果表明，石榴果皮提取物主要是通过抑制HMP途径，导致还原力NADPH缺乏，引起代谢功能障碍，达到抑菌的目的；加药组和正常组金黄色葡萄球菌生长曲线的对比结果表明，石榴果皮提取物能够抑制金黄色葡萄球菌对数生长期的菌体分裂；SDS-聚丙烯酰胺凝胶电泳分析表明，石榴果皮提取物能够抑制菌细胞蛋白合成，尤其对菌体内大分子量蛋白作用最明显。

XPS experiments showed that zirconia sample possesses a 2p electron binding energy of phosphorus at 132.95 eV after the zirconia was treated with DNA solution, which showed that DNA was adsorbed onto this zirconia; TEM images showed that DNA appears in emanant on zirconia particle.

研究了镧离子、铕离子及钙离子对DNA电子传递速率的影响，用Tafel方程计算了动力学速率常数。结果表明，在金属离子的浓度均为1.0×10〓mol/L时，分别加入铕离子、镧离子及钙离子，其动力学常数分别是原来的2.91倍、2.26倍及1.45倍，其增加的顺序是Eu＞La＞＞Ca。

By comparing with the curves of classical antibiotics in Doskochilova system, the antibiotic type of fermentation broths was identified. The antimicrobial tests showed, the inhibition rate of alt the twenty strains were considerably and ten of them reached 80% when the fermentation broths was diluted by 10 times. It showed strongest inhibition effect against Erwinia carotorora (17.75 mm). Organize method tests showed that inhibition rate of fermentation broths of the strain HJ1-2 to Sclerotinia sclerotiorum and Botrytis cinerea was 100% and 71.39%, respectively.

发酵液的抑菌活性测定结果表明，放线菌HJ1-2菌株发酵液对20种供试病原真菌均具有不同程度抑制作用，其10倍稀释液对其中10种病原真菌菌丝生长的抑制率达到80%以上；供试7种细菌中放线菌HJ1-2菌株发酵液对白菜软腐病菌的抑制作用最强，抑菌圈直径为17.75mn；放线菌HJ1-2菌株发酵液对油菜菌核病和番茄灰霉病的抑制率分别为100和71.39%。

The study of morphological, physiological and biochemical characteristics showed that the strain 32-11-2-2 was a slight halophilic strain; it had the morphological characteristics of a continent Bocillus and could produce amylase, fluidify glutin; and it also showed a positive reaction in the litmus milk test. The 16S rDNA analysis showed the strain 32-11-2-2 had a 98% homology with Bacillus subtilis.

结果：分离得到421株细菌，包括活性菌株54株，其中活性菌株32-11-2-2是一株弱嗜盐菌，具有陆地芽孢杆菌的形态特点，能产生胞外淀粉酶、过氧化氢酶，液化明胶、石蕊牛奶反应阳性，16S rDNA序列分析该菌株与枯草芽孢杆菌同源性达到98%以上。

3 Patients were in the acutestage,〓FDG and 〓NH〓 PET showed brain stem infarction in 2 patients, while CT was normal. MRA of one of them showed right vertebral artery obstruction. One case showed focal infarction within the territory of the right middle cerebral artery 12 hours after stroke onset.〓NH〓 uptake was reduced in right frontoparietal lobe, putamen and part of thalamus, while hyperperfusion was observed in ipsilateral head of caudate nucleus and a portion of thalamus.

急性期3例，2例〓FDG和〓NH〓均示病灶位于右侧脑干为放射性缺损区，CT检查均未显示，1例MRA示右侧椎动脉阻塞：另1例〓FDG示病灶在右侧大脑中动脉区域，与12小时CT所示病灶范围相同，但12小时的〓NH〓示右侧额、顶叶，壳核和部分丘脑为放射性减低和缺损区，而同侧尾状核头部和部分丘脑为过度灌注；发病24小时〓FDG显示除〓NH〓所见缺损区外，右侧额、顶叶部分区域和右侧尾状核头部代谢异常增高，血流和代谢有不一致处。

The results showed that though apoHb could be reconstituted with all FePs, it showed different functions. Binding of apoHb with hemin can enhance the heterolytic cleavage of O-O bond of H〓O〓, but inhibiting the cleavage when FeTCPP and FeTPPS〓 were used instead of hemin. The binding of apoHb with FeTTMAPP showed negligible influence on the cleavage of O-O bond in H〓O〓.

结果表明，虽然具有阴离子基团的FeTCPP和FeTPPS〓能够在天然血红蛋白中辅基位置的附近与脱辅基血红蛋白结合，但是由于结构上与天然辅基的差别，使得血红素蛋白中相应的氨基酸残基不能起到有效的作用，因此不但不能提高反而抑制了其过氧化物酶活性；而对于具有阳离子基团的FeTTMAPP，脱辅基血红蛋白对其活性没有明显的影响。

Results 9 cases of retinoblastoma showed the high-density tumor,calcific plaque as well as extraglobal extensions.6 cases of choroidal melania showed the retrobulbar tumor with enhancement.2 cases of coats disease showed the density in the crystalline lens of the eyeball diffuse enhanced and CT volue was from 60 to 88 HU.

结果 视网膜母细胞瘤9例，表现为眼球内高密度肿块、钙斑及球外侵犯。脉络膜黑色素瘤6例，表现为眼球后部肿块，多明显强化。Coat's病2例，表现为眼球玻璃体内密度弥漫性增高，CT值60～88HU。

RESULTS: In this series, 6 cases showed hematemesis, 2 showed melena and 5 showed both symptoms. All cases underwent endoscopic examination after being hospitalized; 11 cases received a positive diagnosis by endoscopy (11/13, 85%), the success rate of first endoscopic therapy was 91%(10/11), and 4 cases were treated by laparotomic surgery (4/13, 31%). Among them, definitive surgery was performed under the guidance of endoscopic hemoclipping in 2 patients who failed to be treated by endoscopy. In another 2 cases, diagnosis was missed by endoscopy and definite diagnosis was made only following exploratory operation or on the basis of pathology after the operation.

结果：13例患者临床表现为单纯呕血者6例，呕血伴黑便者5例，单纯黑便者2例，所有病例入院后均行内镜检查；其中11例经内镜确诊(11/13， 85%)，首次内镜治疗成功率为91%(10/11)。4例经手术治疗(4/13， 31%)，其中2例内镜治疗失败者在内镜钛夹指引下实施确定性手术，另2例内镜漏诊者靠手术探查或术后病理确诊。10例随访患者无1例复发。

Furthermore, out of 497 fAFLP markers, 80 special bands were found to be able to distinguish the four groups from each other and may be applied for germplasm characterization and molecular assistant classification of Meretrix clam.4 Molecular classification of two species of Meretrix clam based on fAFLP and ITS sequences4.1 The results of fAFLP maker analysis of S, G and W showed that each group had their own specific loci among which there were 53 special loci in W group, much more than those of S group (14) and G group (21). Among the 53 loci, nine were all dominant loci. These unique loci could be taken as molecular markers to distinguish W from other groups. The genetic similarity indexes and distance matrix between S and G groups were 0.9585 and 0.0424 respectively, but the genetic similarity indexes and distance matrix between W group and S or G group was 0.7939 or 0.7941, and 0.2308 or 0.2305 respectively. The results revealed that significant difference existed between W and S or G groups in molecular genetic structure. The phylogenetic trees by the methods of UPGMA and NJ also indicated that S and G populations were very closely related, while W population was a relatively independent cluster, lying beyond the species which S and G belong to.4.2 The internal transcribed spacer region of the rDNA from S group, G group and W group were PCR amplified and sequenced. The results showed that the size of ITS ranged between 1266-1269bp in W group, while those in G and S groups were 1614bp and 1520bp respectively. The GC content ranged 62.32-62.62% in W group while it was 61.77% in G group. The genetic distances between three populations of W group were 0.001～0.003, but it was 0.110 or 0.147 respectively between W group and G group or S group. Phylogenetic trees by NJ method also showed that G group was very closely related to S group, while W group was a relatively independent cluster.

在457个总扩增位点中找出了53个W的特有位点，远多于S群体(14)和G(21)群体，而且在53个特有位点中有9个出现频率为100%的位点，这些位点可以作为区分其它2个群体的特征性标记；S– G群体特有的位点有112个，其中有4个位点出现频率为100%，可作为S– G群体区别于W群体的特征性标记。S群体和G群体间的遗传相似性系数为0.9585，遗传距离只有0.0424，在NJ和UPGMA法构建的亲缘关系的树状图上均首先聚在一起，说明二者的亲缘关系很近，应属于种内群体间的关系；而W与S和G的遗传相似性系数均较小(0.7939和0.7941)，相对遗传距离很大而且十分相近(0.2308和0.2305)，在亲缘关系树状图上单独分出一支，也表明W与S和G群体间的亲缘关系较远。4.2 ITS序列比较分析通过对白壳文蛤、山东文蛤和广西文蛤的ITS序列扩增电泳、PCR-RFLP分析和ITS序列分析发现，W的ITS序列长度在1266-1269 bp，而S和与G的ITS序列总长度分别为1520 bp和1614 bp；从ITS1和ITS2长度来看，W分别为739-741 bp和316-317 bp，S为895 bp和414 bp，G为987 bp和416 bp；而从ITS碱基组成来看，W的GC含量在62.32-62.62%之间，而G群体为61.77%。W的3个壳色不同群体间的遗传距离仅0.001、0.002和0.003，S与G群体间的遗传距离是0.010，说明W群体内变异很小，而S与G群体间已出现明显的遗传分化，但还均属于种内群体间的遗传变异；而W与G和S的遗传距离分别达到0.110、0.147，两个类群差异显著，已远超出种内群体间的遗传变异。

Through designing of composition and structure of the bioactive graded coating,innerstress and its distribution in the coating were analyzed and calculated, the resultsshowed that when composition distribution coefficient n was 1.5, a reasonable stressdistribution could be got, that was at the beginning of deposition the suspension containingrichly BG granules was used so that a rich BG granules layer, a good transitional layerbetween BG layer at the bottom and the coating could be obtained at the titanium alloy side,the bottom of the coating; the stress value near the interface and surface and its character,pressure stress or tensile stress, were decided by the character of its composition itself.Changing composition distribution coefficient n could only change the variation tendency ofstress in the coating, but did not change the stress distribution rule in the coating. Thethinner the coating is, the sharper stress variation in the coating is, which does not mean thatthicker coating is better because the thicker the coating is, the little the permitteddeformation of coating is, so the coating thickness should be thinner, for example, about50μm for bending applications, but for applications only bearing pure shear stress, such asroot of tooth implant, the coating can be thicker little, for instance, about 80～100μm. The study on electrification characteristic and electrophoresis deposition of HAand BG granules in aqueous and non-aqueous solution system found that EPD almost didn'toccur in aqueous solution system. However, because HA granules take position charges inabsolute alcohol, a homogeneous EPD be carried out on the cathode titanium alloy slice, but taking negative charge in absolute alcohol the BG granules not be deposited on the cathode. A guided HA crystallizing, 100～300nm, on surface of the BG granules be realized by metathetical reaction, which cover BG granules with HA microcrystals and make the covered BG granules taking position charges in absolute alcohol, sequentially realize the EPCD of the BG and HA granules on the cathode, so it is feasible to make a titanium alloy/BG/HA bioactive graded coating by making use of EPCD technology. The corrosion experiment of rich boron bioglass coating and plasma spray coating showed that split phase, rich boron and rich silicon phase, occurred during its preparation. In basic medium the corrosion behavior of 〓 BG coating showed uniformity corrosion, the corrosion mostly occurred at rich boron phase area, therefore batch formula design of BGshould avoid the occurring of split phase. The corrosion appearance of plasma spray coatingappeared a non-uniform corrosion, mostly occurred at the edge of the laminated HA moltendrops, and emerged an accelerated corrosion tendency, which will easyly lead to corrosioncrackles extending to the interface and the happening of osmotic interfacial corrosion, thatmay be one of the major reasons leading to the coating cracking-off in the later period. Thetesting results of thermal expansion coefficient of 〓 and 〓BG showed the thermalexpansion coefficient of 〓 BG matched with that of titanium alloy better, and 〓 BG couldsinter with titanium alloy into densification enamel layer at low temperature (720℃).

将Ti6Al4V合金在1000℃下进行真空热处理会降低其力学性能，且合金内的V元素会向表面富集，因此，钛合金真空热处理和表面涂层的烧结温度不能过高，即应低于其相转变点；通过对生物活性梯度涂层的组成和结构的设计，分析和计算了梯度涂层内的应力大小和分布，结果表明：对于本研究，当成分分布系数n=1.5时，可以获得较合理的涂层力学性能，即在沉积开始时，采用富含BG颗粒的悬浮液，以便在钛合金侧获得同底层BG有良好过渡的富BG涂层；梯度涂层界面和表面的应力大小、性质由材料组成本身的性质决定，改变成分分布系数，只能改变涂层内应力变化的趋势；涂层的厚薄不影响涂层内的应力分布规律，但涂层越薄，涂层内的应力变化越快，但这并不意味着涂层越厚越好，因为涂层越厚，涂层允许的变形越小，对于应用于弯曲受力部位的涂层而言，涂层应薄一点为好(50μm)；而对于仅纯受剪切应力的部位，如牙根种植体，涂层可适当加厚(80～100μm)；通过对HA和BG颗粒在水溶液体系和非水溶液体系中的带电特性和电泳沉积的研究发现，它们在水溶液体系中很难发生电沉积；在无水乙醇溶液中，HA颗粒带正电，可在阴极钛合金片上发生均匀的电泳沉积，而BG颗粒则带负电荷；利用复分解反应法，可以制得100～300nm的HA，通过诱导HA在BG颗粒表面结晶，可对BG颗粒进行表面包覆，获得了被HA包覆的BG颗粒，改变了BG颗粒表面的带电特性，使BG和HA颗粒在无水乙醇中均带上正电荷，从而实现了HA和BG颗粒在阴极上的共沉积。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。