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Ultrasonic examination of cancerous hydrothorax showed that hydrothorax in thoracic cavity had no echo zone filled with full of thin light spots;sound transparent was not very clear;pleura showed uniform thickening;opaque dark area of fluid had parenchymatous uniform phyma.

结果 结核性胸腔积液超声检查显示胸腔内胸腔积液无回声区透声清,胸膜均匀性增厚,液性暗区内未见实质性肿块;癌性胸腔积液超声检查显示胸腔内胸腔积液无回声区内充满细小光点,透声欠清,胸膜呈非均匀性增厚,液性暗区内可见有实质非均质性肿块。

The contents of mineral elements, like Fe, in LMTCMs were higher than that of nor-LMTCM. The result of analysis of mineral element of 14 LMTCMs by ICP-MS method also showed the content of Fe have something to do with lung meridian tropism effects. The result of the study of platycodon radix approved the laws too. These laws were used to predict if the marine medicines have the lung meridian tropism effects.2. The Compatibility laws of Medicines and their chemical composition in LSP were studied by mining the data of the constituent herbs and their chemical composition. The results showed that the most constituent medicines of lung system prescriptions have lung meridian tropism. Radix glycyrrhizae, herba ephedrae, semen armeniacae amarum, fructus schisandrae Chinese and their compatibility are common in lung system prescriptions. Terpene, alkaloid, steroid, and flavanoid compounds and their compatibility are common in lung system prescriptions.3. The marine phycophyta medicines of LSP were studied by the same method.

对比归肺经中药及肺系方剂主要化学成分构成,萜类、生物碱、甾体类成分可能是归肺经中药及肺系方剂的主要药效成分;结合君臣佐使药的化学成分构成特点及肺系方剂组成中药的归经构成情况,肺系方剂组方设计应该考虑以下几个方面:一、优先考虑归肺经中药,在应用不归肺经中药时要配伍归肺经中药;二、组方时应考虑含萜类、甾体及生物碱类化合物的中药;治疗肺咳病及咳嗽症状时可以考虑含萜类化合物的中药,治疗气喘症状时可以考虑含生物碱类化合物的中药;三、在确定组方中药或化学成分配伍关系时,君药多考虑含生物碱、黄酮类化合物的中药;臣药、佐药可以考虑含萜类及甾体类化合物中药;使药可以考虑含萜类、甾体类及黄酮类化合物的中药。

After induced with IPTG, the proteins expressed by different fragments were analyzed by SDS-PAGE, and the results showed that all these truncated proteins could be expressed in E. coli BL21. Bioassay result against Plutella xylotella showed that the active fragment of Cry1Ba3 protein was located between amino acid No. 22 and No. 655. As the insecticidal activity of the truncated protein containing amino acid No. 54-655 was declined significantly, and No. 22-627 and No.

采用浸叶法检测这些蛋白片段对小菜蛾的杀虫活性,研究结果表明含有第1~685位和含有第22~655位氨基酸的蛋白片段对小菜蛾的毒性,与全长Cry1Ba3蛋白相比,没有改变;含有第54~655位氨基酸的蛋白片段对小菜蛾的毒力明显降低;而含有第22~627位和含有第85~655位氨基酸的蛋白片段完全丧失了对小菜蛾的活性。

Result: Porosis and inflammatory cell infiltration in the spinal cord, rupture of muscle bundles, hypertrophia and over growth of muscle cells and collagens, and inflammatory cell infiltration in the bladder wall could been seen under HE dyeing. Nissal dyeing showed the decrease of neurons at the injured segment, upper and lower segments of the spinal cord. Result of immunohistochemistry showed there were caspase-3 positive expression in all the segments. The injured spinal cord and bladder expressed significantly more caspase-3 positive results (P.0l), upper and lower segments were lower than the injured segments (P.01), but higher than the control team (P.0l).

结果:HE染色发现脊髓损伤后脊髓内空洞形成、炎性细胞浸润等病理学变化,膀胱壁中可见到肌束断裂、肌细胞肥大增生、胶原增生、炎性细胞浸润等病理学改变;Nissal染色显示脊髓损伤后损伤节段及其上、下节段均出现神经元数量的减少;免疫组化结果表明3组均有不同程度的caspase-3表达,损伤组脊髓和膀胱内膜caspase-3表达较正常组显著增加(P.01),损伤上段及下段caspase-3表达较损伤节段为少(P.01),但均高于正常对照组(P.01)。

All 31 YSTs (5 pediatric and 26 postpubertal) showed strong positive SALL4 staining in more than 90% tumor cells but had negative OCT4 staining. Both spermatocytic seminomas showed positive SALL4 staining in 80% to 95% tumor cells in all 3 types of tumor cells with weak-to-moderate staining intensity.

为了检验SALL4的特异性,我们还给23例睾丸的非GCTs(10例间质细胞瘤、4例支持细胞瘤、3例腺瘤样瘤、3例睾丸旁横纹肌肉瘤、2例弥漫性大B细胞瘤、1例睾丸网的乳头状囊腺瘤)和275例非睾丸性肿瘤(158例转移癌、12例转移性黑色素瘤、11例原发性和2例转移性间皮瘤、72例原发性和20例转移性肉瘤)做了SALL4免疫染色。

The aim is to use rat model of formalin result to pain to observe rat praxiology responses after giving ASA , The results found that: The rats showed spontaneous pain-related responses after formalin test. After being injected formalin into one hindpaw, the rats of F group immediately showed intense pain-related response: flinching / shaking and licking / biting injected paws.

在福尔马林炎性痛动物模型测定给药后大鼠的痛行为反应,发现ASA可以明显抑制F引起的双相痛反应,故从行为学的水平确定了ASA有显著的镇痛效应。

Result: Compared to normal subjects, some functional regions associated with encoding, maintenance and retrieval process showed increased activation in schizophrenia patients, i.e. right precuneus for encoding process, left PMA, left DLPFC, right precuneus and left VLPFC for maintaince process, left PMA for retrieval process. In addition, subcortical structures, primary motor cortex and some verbal regions in left temporal lobe also showed more activation.

结果:与正常组比较,患者组工作记忆不同认知过程的执行脑区激活程度增加,编码期激活增加脑区为右侧楔前叶,维持期激活增加脑区为左侧PMA、左侧DLPFC、右侧楔前叶及左侧VLPFC,提取期激活增加脑区为左侧PMA,而且患者组还激活了更多皮层下结构、初级运动皮层及左侧颞叶语言相关脑区。

The reseach on activity changes of SOD, POD and CAT during the somatic embryogenesis of Y35 showed:(1) The activty of SOD was from 52.98 to 133.20 U·g-1·h-1, and remained a rising trend after early single embryo forming, this revealed that SOD might be positively correlated to the differentiation of embryogenic cell and the development of somatic embryo.(2) The activty of POD was from 0.05 to 0.50 U·mg-1·min-1, ascended firstly and desceded later, and was highest in embryogenic callus and lowest in late single embryo , this revealed that POD might be positively correlated to the division and differentiation of proembryo mass, while negatively correlated to the development of PEMⅢto late single embryo.(3) The activty of CAT was from 0.86 to 2.81 U·mg-1·min-1, showed an up-down-up trend, reaching to the highest peak at the time of early embryo formating and decreasing to the lowest at the time of early cotyledonary embryo formating, this revealed that CAT might be positively correlated to the development of early single embryo, while negatively correlated to the formation of middle single embryo and early cotyledonary embryo.The changes in activty of SOD, POD and CAT indicated these three antioxidant enzymes coregulated the differentiation and development of embryogenic cells during Larix somatic embryogenesis.4. Differentially expressed cDNA libraries of the stages of proembryo mass and somatic embryo maturation were successfully constructed by suppression subtractive hybridization.

对Y35体细胞胚胎发生过程中抗氧化酶活性变化的研究显示:(1)SOD活性在52.98~133.20 U·g-1·h-1之间,并在早期单胚形成后一直保持上升的趋势,表明其与胚性细胞的分化及体细胞胚的发育均具呈正相关;(2)POD活性在0.05~0.50 U·mg-1·min-1之间,呈现出先下降后升高的趋势,在胚性愈伤组织中最高,而在后期单胚形成时降至最低,表明其与原胚团的分裂和分化呈正相关,但与PEMⅢ向后期单胚的发育呈负相关;(3)CAT活性在0.86~2.81 U·mg-1·min-1之间,表现出升-降-升的变化趋势,在早期单胚形成时升至最高,在早期子叶胚形成时降至最低,表明其与早期单胚的发育呈正相关,而与中期单胚和早期子叶胚的发育呈负相关。

In bioconv ertion experiment for spiramycin of No.5 transformant, the bioconversion product was analysed with TLC, bioautography, HPLC and mass spectrum.Results showed that the bioconvert product is propionyspiramycin. Propionyl acylase gene was l ocolized on 0.53 kb insert DNA fragment of recombinant pasmid pLJM95. Analysis result of DNA sequence showed that content of G+C is 68.2% for 0.53kb insert DNA fragment .

以No.5转化子对螺旋霉素进行生物转实验,其转化产物经薄层层析,生物显迹,高压液相色谱及质谱分析表明,与标准丙酰螺旋霉素一致,这说明 No.5 转化子具有将螺旋霉素生物转化为丙酰螺旋霉素的能力,丙酰化酶基因位于重组质粒,pIJM95 0.53kb 插入DNA片段上。

Sequence analysis showed that the ITS gene was proved to be a complex structural region which was not suitable for the identification of Prototheca species, but the LSU and SSU rDNA regions showed 94﹪ and 99.9﹪ sequence identities with Prototheca zopfii var.

核酸序列分析显示核糖体内转录间区DNA可能存在复杂结构,不适合无绿藻的测序鉴定;但核糖体大亚基及小亚基DNA序列分析,与祖菲无绿藻碳水化合物变种(Prototheca zopfii var.hydrocarbonea)一致性分别为94﹪、99.9﹪。

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I Showed Her
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