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Results: Identification of the Ad-pml by restriction digestion and PCR showed the length. Ad-pml was confirmed in sequence. fluorescence showed successful recombined adenovirus with infectiveness.

结果：经PCR反应可扩增出1767bp大小的片段，测序结果检测Ad-pml序列正确，通过免疫荧光反应证明病毒包装成功并且具有感染力。

Results The anesthesia range was narrowed and anesthesia duration was shortened with the prolongation of epidural catheter placement,Epidural roentgenography showed that the medium diffused normally within epidural space,biopsy of epidural tissues showed that there were inflammative cells infiltration and fiber proliferation with longer epidural catheter placement（more than 4 weeks）,without significant differences between both groups.Conclusion Epidural corticosteroids can not prevent epidural adhesion from occurrance induced with long-term epidural catheter placement.

结果 随着硬膜外腔置管时间延长，麻醉阻滞范围变窄，麻醉阻滞时间也明显缩短，两组之间没有明显区别；硬膜外腔造影显示：造影剂在硬膜外腔扩散良好，无明显受阻；组织学检查显示：置管时间长(4周以上)的动物，硬膜外组织炎细胞浸润、纤维组织增生、硬膜增厚，而且随置管时间延长，这&种变化更&加显著，但两组之间没有明显区别。

Results:Conventional MR showed infraspinatus abnormalities were four cases,Mrarthrography showed infraspinatus abnormalities were seven cases.

回顾性分析经关节镜证实的8例肩关节内在撞击综合征常规MR成像和MR关节造影检查资料。

Results Of 10 cases with internal impingements,MR showed infraspinatus abnormalities were 8 cases.Supraspinatus abnormalities were 4 cases.Labral abnormalities were 9 cases.Posterosuperior humeral head damage were 10 cases.10 cases with subacromial impingement.MR showed infraspinatus abnormalities were 2 cases.Supraspinatus abnormalities were 8 cases.Labral abnormalities were 0 case.

结果 10例内在撞击综合征MR表现为冈下肌腱异常者8例，冈上肌腱异常者4例，盂唇异常者9例，肱骨头后上部异常10例。10例肩峰下撞击综合征MR表现为冈下肌腱异常者2例，冈上肌腱异常者8例，盂唇异常者0例，肱骨大结节异常8例。

It has been found that a synthetic resveratrol analogue showed good anti-breast and anti-colon cancer activity, while no effect on normal cells. The animal experiment is underway and showed inspirable result.

在此基础上合成了一个白藜芦醇衍生物，它对乳腺癌细胞和结肠癌细胞有很好的抑制作用，但对正常细胞没有影响，正在进行动物实验，已取得令人鼓舞的结果。

The results showed:(1) MP polymerase chain reaction test showed positive results in the bronchoalveolar lavage fluid from all of the MP-infected rats (infectious group, n=4), while they were all negative in BALF from the control group (n=4,P＜0.05) and from those rats both infected with MP and, at the same time, treated with erythromycin (treating group n=4,P＜0.05). Bacterial cultures of the bronchial and lung tissue were negative in all three groups. Using transmission electron microscope the widened interalveolar septa with increased amount of collagen were found in the infectious group, while there were no obvious abnormalities in the other two groups.

结果显示：(1)感染组动物(n=4)支气管肺泡灌洗液肺炎支原体-PCR检测均为阳性，而对照组(n=4)和感染加红霉素治疗组动物(n=4)均为阴性(P＜0.05)；三组动物的支气管和肺组织常规细菌培养结果均为阴性；感染组动物透射电镜检查见肺泡间隔增宽，其中有较多胶原纤维堆积，其余两组则未见明显异常。

Several anti-tumor cell lines and carbonic anhydrase Ⅱ model were established. More than 10000 compounds and extracts from plants were screened using these models and about 10 compounds showed strong inhibitory activities. Two species of plants Inula helianthus-aquatica and Saussurea deltoidea were chosen to investigate chemical and bioactivity constituents based on the results of screening of extracts and fractions. 58 compounds were isolated and identified from these two plants, 6 compounds were found to be new and 14 compounds showed anti-tumor activities. We also studied the anti-tumor mechanism of the compound bigelovin isolated from Inula helianthus-aquatica. Bigelovin was found with apoptotic activity against cell line U266 by lowering the amount of important transcription factor E2F-1 which inhibits the cell cycle at G0/G1 stage. Docking study on 7 natural new phenolic inhibitors of CA Ⅱ and 3D-QSAR study on 61 sulfonamide inhibitors of CA Ⅱwere also investigated. The calculated results were in good correlation with experimental results.

基于以上两种重要疾病和研究组方向，本论文工作开展了多个肿瘤细胞株和碳酸酐酶Ⅱ靶标模型的建立和筛选工作，累计筛选1万多个/次样品，发现10余个活性较强化合物；从粗提取物筛选结果中选取两种抗肿瘤活性强的菊科植物水朝阳旋覆花(Inula helianthus-aquatica)和三角叶凤毛菊进行了系统化学和活性成分研究，分离鉴定了58个化合物，发现6个新化合物和14个抗肿瘤活性化合物；通过合作对水朝阳旋覆花中活性较强的bigelovin进行了抗肿瘤作用机制研究，发现它可能是通过降低G2期重要的转录因子E2F-1，致使细胞周期阻滞在G0/G1期，引起多发性骨髓瘤U266细胞凋亡；开展了7个新的天然酚类CAⅡ抑制剂的对接研究和61个合成的系列磺酰胺类CAⅡ抑制剂的3D-QSAR分析，结果表明计算与实验一致。

A strain of breast tumor cell line ZHL2006 was established and biologically identified. The ZHL2006 cells grew fast in vitro and the population doubling time was 43.6h. Electronmicroscopical observation showed that there were microvilli on the surface of the cells, nucleus irregularity, karyoplasmic ratio increased, nucleolus distinctness, et al.. Chromosome number and structure were abnormal. Immunohistochemistry showed that PCK were expressed. The ZHL2006 cell line could form onto clone in soft agar and had oncogenicity in athymic mice.

ZHL2006细胞在体外生长迅速，群体倍增时间为43.6h；倒置相差显微镜及Giemsa染色镜下观察细胞为多边形及梭形；透射电镜观察可见细胞表面有微绒毛，核高度不规则，核浆比例增大，核仁明显等；染色体数目和结构异常；广谱细胞角蛋白染色阳性，说明其自上皮而非来自基质；同时ZHL2006细胞系在软琼脂中可形成克隆，具裸鼠致瘤性。

In group B, a large amount of inflammatory cell infiltration in liver tissues were noted and hepatic cells showed spotty necrosis and bleeding under the light microscope; the electron microscope showed a significant decrease of mitochondria cristae, the broadness of endoplasmic reticula, hepatic cell necrosis and karyopyknosis.

B组光镜下可见肝组织大量炎性细胞浸润，肝细胞点状坏死及出血；电镜下可见线粒体嵴明显减少，内质网扩张，出现凋亡小体及核固缩。C组上述病理变化明显减轻。

Results HE staining showed that sulindac could inhibit the growth of tumor cells, with the morphological presentations as apoptosis and necrosis including pyknosis, karyorrhexis and chromatin crescent formation, etc. By electron microscopy, cells also had apoptotic characteristics including chromatin crescent formation and apoptotic body. Immunohistochemistry and Western-blot assay results showed that 48 hours after sulindac administration, the expression of NF-κB, bcl-2 and bax gradually decreased, while the level of bax protein gradually increased, in a dose dependence manner.

结果 苏木精－伊红染色检测结果显示舒林酸抑制肿瘤细胞生长，肿瘤细胞出现核固缩、核碎裂、染色质边集等凋亡及坏死的形态学表现；电镜下可看到染色质边集、凋亡小体；免疫组化、Western-blot结果显示舒林酸作用48 h后人胃癌SGC-7901细胞NF-κB、bcl-2表达逐渐下降，bax表达逐渐上升，具有剂量依赖性。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。