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Results Unvaried non-conditional logistic regression analysis showed that sex,primary disease,site of puncture,nursing care,and ways of use of the catheter showed no correlation with CRS.

先以单因素非条件logistic回归分析筛选出有统计学意义的危险因素,再应用多因素非条件logistic回归分析患者CRS形成的独立危险因素。

Morphological and physiological identification showed that NTG-01 was Gram-negative, short rod shape, with flagella around the bacteria, V-P test positive and Methyl red test negative. The bacterium can use glucose as carbon suource and produce gases. No H2S produced on TTS medium. Lysine decarboxylase and orthinine decarboxylase, catalase and gelatine test are positive. Arginine decarboxylase, dehydrolase, oxidase and urase test are negative. Sequencing and alignment of 16S rDNA showed that it shared a 99% homologous sequence with Enterobacter aerogenes.

结果显示菌株NTG-01为细菌,革兰氏染色阴性,短杆状,鞭毛周生,V-P试验阳性,甲基红实验阴性,利用葡萄糖产酸产气,不能在TIS培养基上产生H_2S,赖氨酸和鸟氨酸脱羧酶试验呈阳性,精氨酸脱羧酶和双水解酶试验呈阴性,氧化酶和脲酶试验阴性,接触酶和明胶液化试验阳性,但后者反应迟缓,一周后才观测到结果。

The result of uroscopy of the proband was strong positive. There was a novel deletion mutation of c.876-877 del TC in the coding region of exon 6 of IDS gene, which was a hemizygous mutation. However, the mutation of his mother and sister was a heterozygous mutation. Detection of the exon 6 of IDS gene showed that the mutation was not found among normal controls and other patients with MPS I, IV, and VI other than MPS II. Homology comparison of amino acid sequences from different species showed that the phenylalanine glutamine of the mutation site of c.876-877 del TC located in p.292-293 was highly conserved. The activity of IDS enzyme of the proband was only 2.3 nmol/4 h/mL, which was much lower than normal; but the activity of IDS enzyme of his father, mother and sister was 641.9 nmol/4 h/mL, 95.8 nmol/4h/mL and 103.2 nmol/4h/mL, respectively.

结果显示:先证者尿检呈强阳性;其IDS基因exon 6编码区内存在c.876-877 del TC新缺失突变,为半合子突变,而其母、其姐为杂合突变;正常对照和其他非II型MPS患者的IDS基因exon 6的检测结果均未发现该突变;不同物种氨基酸序列的同源性比对显示: c.876-877 del TC突变所在的位置即p.292-293的苯丙氨酸谷氨酰胺高度保守;酶活性测定的结果显示:先证者的IDS酶活性仅为2.3 nmol/4 h/mL,大大低于正常值,而其父的为641.9 nmol/4 h/mL,其母的血浆酶活性为95.8 nmol/ 4h/mL,其姐的为103.2 nmol/4 h/mL。

On electrolyte disturbances, for those who were not given diuretic showed low serum Na+ or low both Na+and Cl-; for those who had taken diuretic showed variform.

随心力衰竭程度的加重,老年CHF病人电解质紊乱的发生率增加,且以低Na+、低Na++低Cl-为主,尤其是未用利尿剂病人。

On electrolyte disturbances, for those who were not given diuretic showed low serum Na(superscript +) or low both Nat and Cl(superscript -); for those who had taken diuretic showed variform.

未用利尿剂病人电解质紊乱全部为低Na或低Na+低Cl,而应用利尿剂的病人电解质紊乱表现多样。

Sample collection: Two hundred and fifteen sera samples of normal population were collected from the peripheral blood of healthy blood donors of Shenyang, and the sera were separated by centrifugation after blood clotting; The patients sera were collected from the outpatients and inpatients of No. 1 hospital of China Medical University and the Second hospital of Jilin University. Syphilis was confirmed by clinical and serum test of TPHA and RPR; That of pemphigus vulgarism was confirmed not only by clinical, histopathological examination, but also by direct and indirect immunofluorescence test. DIF test on lesion or non-lesion skin showed immunoglobin and/or C3 deposits in the intercellular space of epidermis, and IIF test showed positive anti- intercellular antibody in patient serum using mouse's or rat's tongue or esophagus as substrate.

标本的收集:正常人的血清:采集沈阳市正常献血员外周静脉血共215份,待血凝后离心分离血清;患者的血清:来自中国医科大学一院及吉林大学二院门诊及住院部的病人,梅毒患者除了病史及临床表现外,全部经血清学TPHA及RPR确认;寻常性天疱疮患者除了临床及组织病理诊断外,还进行了直间接免疫荧光的检测,直接免诊断疫荧光:在皮损区及非皮损区的表皮细胞间显示IgG和/或C3补体沉积;间接免疫荧光用鼠或兔的舌或食管作为底物,在病人的血清中显示有肯定的细胞间抗体。

Primer 325 RAPD showed all COS-PA from SICU in 2003 were identical (SICU-A type), but the Burn-A type and SICU-A type were distinctly different. Most of the non-COS-PA from SICU also showed the trend of prevalence, which was defined as SICU-B type in RAPD typing, including the strain found in the bottom of the washstand.

SICU 2003年收集的COS-PA,经RAPD(引物325)分型为同一型别;在SICU收集的non-COS-PA也多数呈同一型别,自SICU水池下水口收集到的1株non-COS-PA也属于此型。

The 54 accessions were classified into five major groups:Ⅰ,Ⅱ,Ⅲ,Ⅳ and Ⅴ by cluster analysis using UPGMA, which showed significant relationship with the origin regions of accessions. Genetic differentiation between and within eight eco-geographical groups of C.dactylon was estimated by Shannon's diversity index, which showed that 63.81% genetic variance existed within group, and 36.19 % genetic variance was among groups, indicating rather large genetic distances among those germplasm, and there was a correlation between genetic differentiation and eco-geographical habits among the groups.

聚类分析结果表明,52份材料可聚为5类;基于Shannon多样性指数估算了8个狗牙根生态地理类群内和类群间的遗传分化,类群内的遗传变异占总变异的63.81%,类群间的遗传变异占总变异的36.19%,表明这些抗源材料遗传差异较大,各生态地理类群间的遗传分化与其所处的生态地理环境具有一定的相关性。

The SOD and the LDH-1 activities and EST (R_f=0.17 and R_f=0.72) at 1℃weresignificantly higher than the values of the room temperature(P<0.05), but the total enzymeactivities of LDH, G6PDH and EST at room temperature and 1℃were similar(P>0.05).This showed well-developed anoxia tolerance of the Heilongjiang wood frog to maintainplasma viability when low temperature interrupts the delivery of oxygen and substrates. LDH-1rose significantly avoided the liver lactic acid accumulation that may created the liver to bewearied. EST (R_f=0.17 and R_f=0.72) rose may enhance the ester metabolism to offer muchenergy. These showed steady metabolic rate and relative stable organism environment of theHeilongjiang wood frog to survive under low temperature.Native PAGE separated proteins of liver tissue successfully on the better condition is 6%final gel concentration and there were 15~19 bands identified on this condition.

同功酶电泳图谱显示,1℃组与室温组黑龙江林蛙肝脏LDH酶活性差异不显著,均以LDH-5为主,但1℃组LDH-1显著高于室温组(P<0.05),推测黑龙江林蛙为适应低温低氧环境,增强LDH-1酶活性以较好的利用乳酸,避免因大量乳酸形成造成肝脏疲劳;而1℃组肝脏G6PDH酶活性无显著变化,进一步说明低温条件下葡萄糖戊糖途径和糖酵解作用的互相协调,维持机体代谢的稳定;1℃组和室温组EST总酶比活力无显著变化,但1℃组中R_f=0.17和R_f=0.72的EST同工酶显著升高(P<0.05),推测在低温条件下黑龙江林蛙可能通过选择性增强部分酯酶同功酶活性,增加肝脏酯类物质代谢,为机体提供维持生命必需的最低能量。

The results showed that the TiN andN films are polycrystalline phase. The properties and structure of TiN films are mainly determined by the N2 mass flow rate, and as the N2 mass flow rate increases, the structure of the TiN films transforms to f.c.c. type leading to better the properties of films. The presence of negative bias voltage can optimize the grain of TiN films, lower the defect proportion and denser the films, which improves the hardness of films. The TiN films conform to the free carrier absorption mechanism and there are many of free electrons with lower N content in TiN films. With the increase of N content, the quantity of free electrons and reflectivity of films decrease, and the plasma frequence shifts to the lower energy, which leads to the regular change of colors of films from silver, yellowy, golden to yellow-red, meanwhile the lightness of films decreases. The goldenN film consists of TiN and ZrN phase, but belongs to a sigle f.c.c. structure with (111) preferred orientation. The Zr-doping dosen't change the position of the valance band, conduction band and forbidden band ofN film, but leads to the presence of new energy levels, which is the reason thatN film remains golden. The transparent hard films with good corrosion resistance and high hardness have been prepared and the further reseachs showed that the grain size of those films is by far smaller than the wave length and the width of forbidden band of those films is very broad, is the reasons that those films are transparent.

研究表明:氮化钛和N 薄膜为多晶态,氮流量决定了氮化钛薄膜的结构和性能,增加氮流量能使氮化钛薄膜的结构向面心立方结构转变,从而得到性能良好的氮化钛薄膜;施加负偏压能优化氮化钛晶粒和减少薄膜中的缺陷,使膜层变得更致密,从而提高薄膜硬度;氮化钛主要遵循自由载流子光吸收,氮含量较少时薄膜中的自由电子数目较多,随着氮含量的增加,薄膜中的自由电子数目不断减少,反射率逐渐降低,等离子体频率向低能端移动,从而使薄膜颜色出现规律变化,由金属色银白色到淡黄、金黄再到红黄,并且薄膜亮度呈下降趋势;金黄色的N 薄膜中存在TiN 和ZrN 的分离相,但其为单一的面心立方结构并具有(111)面择优取向;相对于TiN 薄膜,Zr 掺杂后,并没有使薄膜的导带、价带和禁带发生变化,只是在TiN 禁带内增加了新能级,这也正是掺杂Zr 后,薄膜仍

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I Showed Her
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