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The EAE rats were induced by injecting subcutaneously an emulsion of GPSCH and CFA. In addition, each rat received an intracutaneous injection of pertussis. Albumin in serum and CSF samples was examined on the days of 4,6,8,10,12,14,16,18 and 20 p.i.. The integrity of BBB was assessed by caculating CSF to serum albumin quotient. At the same time the brains, spinal cords, thymus and inguinal lymph nodes were removed for paraffin sections cutting. These sections were used for HE staining and ICAM-1 semiquantitative immunohistochemical staining. The results showed as follows:1. None of EAE rats on days 4,6 and 8 p.i. showed clinical signs whichbecame apparent on day 10 p.i.(2.33±0.76; n=6). On days 12 and 14 p.i.

检测p.i。第4天、6天、8天、10天、12天、14天、16天、18天、20天血清和CSF中ALB含量,其CSF/S ALB比值作为评价BBB损害的指标;同时段取动物脑、脊髓、胸腺和腹股沟淋巴结制成石蜡切片行HE染色和ICAM-1半定量免疫组化分析,结果显示如下: 1、EAE大鼠p.i.4-8天无任何临床表现;p.i.10天症状开始明显,症状评分为2.33±0.76(n=6);p.i.12-14天为发病高峰期,症状评分为3.75±0.54(n=12),病情由最初的活动减少,体重减轻逐渐发展为肢体无力、瘫痪,共济失调,甚至濒死状态;p.i.16天大部分动物症状开始缓解,评分为2.08±0.66(n=6);p.i.20天疾病症状完全消失。

Results In treated group,68 cases showed excellent,13 responsive and 3 irresponsive;in the control group,56 showed excellent,11 responsive,5 irresponsive.

结果 对照组显效56例,有效11例,无效5例;治疗组显效68例,有效13例,无效3例,两组比较差异无统计学意义(u=0.2896,P>0.05)。

It can be show different characteristics in the MRI of the orbital diseases, the sheath of the orbital tumor contain certain characteristics, can express soft muscle locate the orbital compartment or outside. and the tumor showed hypointensity or hypo-or isointense mass on T1-weighted images, and iso-or hyperintense or hyperintense mass on T2-weiglited images, inflammation expresses for the orbital inside limit the soft muscle or widespreads excrescent signal, and showed hypo-or isointense mass on Ti-weighted images, and iso-or hyperintense mass on T2-weighted images, the companion contain fatty the eye muscle big or the eye wreath increase thick, the lachrymal gland is swollen.

其在MRI表现各具有影像学特征,眼眶肿瘤多表现为位于眼眶内、外的局限性软组织肿块,MR T1WI呈等或稍低信号,T2WI呈高或等信号多见;炎性假瘤则表现为眶内局限性软组织肿块或弥漫性异常信号,MR T1WI呈低信号,T2WI呈高信号,常伴有眼肌肥大或眼环增厚,泪腺肿大等。

Results 9 latissimus dorsi flaps survives well.but 1 flap showed small necrosis at the distal part,and all patients showed good profile and function of face and neck.

结果:9例背阔肌肌皮瓣游离移植后完全存活,1例背阔肌皮瓣远端部分坏死,术后患者的面颈部功能和外形得到明显的改善。

In over-wintering species experiment, AMF colonization showed significant difference, which was greatiy dependent upon the plant species character. The AMF colonization rate and index of LaHumperenne in all community in which GnapbaHum affim existed showed better colonization than that in Latium perenne mono

存在有鼠曲草的含有黑麦草的各种组合(如黑麦草+鼠曲草组合,)里黑麦草的AN田侵染指数和侵染率均高于黑麦草单种,、(黑麦草+野豌豆+鼠曲草)等处理中的黑麦草的入MF感染情况表现比较复杂,、(黑麦草+野豌豆+鼠曲草)中黑麦草的AN正侵染指数发生显著性变化P

Cognition disturbance and allolalia were the chief clinical manifestations. The imageology showed that the impairment involved occipital lobe and temporal lobe most. 17 cases presented red-ragged-fibres and parking lot-like ultrastructure in 3 cases. Brain biopsy showed layerage structure. There were 15 of them carried mtDNA A3243G mutation. Conclusions MELAS Syndrome usually involved central nerve system and skeletal muscle.

主要临床表现为认知功能障碍及言语障碍,头颅影像学检查示枕叶、颞叶脑回受累为主。18例行肌肉病理检查显示17例存在破碎样红纤维,3例行肌肉电镜检查发现线粒体堆积呈停车场样排列,3例脑活检示病变皮层组织呈分层样改变,15例发现mtDNA A3243G点突变。

The results indicated: the growth features of different size of tomato fruits were much different;the increasing course of small tomatoes showed "ladder" model,and that of big and middle ones showed "wave" model.The daily change of leafstalk stem-flow velocity presented "double-apex" model.

监测结果表明:由于番茄果实的个体差异和前期生长的差异,大、中、小番茄果实的生长规律有明显的差异;小番茄的增长过程呈"阶梯"型,大中番茄的增长过程呈"波浪"型。

The molecular weight of broad bean vicilin and legumin measured by gel filtration chromatography were 135000 and 351000, DSC showed that their denature temperatures were 72℃ and 87℃ respectively. The legumin had a lowest solubility at pH 4. 5-5. 0 whereas the vicilin at pH5. 5. Vicilin and legumin were all possessed low levels of α-helical and high β-sheet secondary structure fractions. The total content of α-helical,β-sheet and β-turn fraction of vicilin and legumin reached to 90% and 78% separately showed that they had a high ordered structure in solution.

对两种组分的凝胶层析表明7S和11S的相对分子质量分别为135000和351000;DSC分析显示其热变性温度分别为72℃和87℃;溶解度分析表明:11S球蛋白在pH4.5-5.0之间,7S球蛋白在pH5.5时具有最低的溶解度;圆二色性分析结果显示7S和11S球蛋白的β-折叠结构含量分别高达40.5%和38.1%,证明二者都属于β-折叠型蛋白质,二者的α-螺旋、β-折叠和β-转角结构的含量总和分别达到了90%和78%,从而说明它们在溶液中的结构是高度有序的。

The deduced amino acid sequence showed a high identity to the reported sequences of GOBPⅠ from other lepidopteran insects, and showed that SlGOBPⅠ may belong to the family of GOBP.

SlGOBP Ⅰ氨基酸序列与鳞翅目昆虫的气味结合蛋白氨基酸序列具有较高的相似性,表明SlGOBPⅠ属于GOBP家族。

Prior administration of "Styrax Pill for Coronary Disease" proper (6 components viz., styrax liquidus, Borneolum, Lignum santali, Aristolochia, Olibanum and Cinnabaris) or the mixture of Styrax liquidus, Borneolum, Lignum Santali and Olibanum to mice prolonged the survival time of the animals subjected to low pressure hypoxia. Styrax liquidus and Borneolum administered separately showed the same effect, whereas none of the other four components showed significant protection.

用小鼠耐缺氧试验和麻醉狗冠状窦血流量及心脏动-静脉血氧差(MA-VO_2)的实验研究冠心苏合丸及其成分的作用,发现:(1)冠心苏合丸能延长小鼠耐缺氧的时间;(2)冠心苏合丸能使心肌梗塞狗的CSF回升,减少心率和MA-VO_2,对非心肌梗塞狗不能提高CSF但亦能减少心率和MA-VO_2;(3)在本实验条件下,苏合香脂和冰片似为冠心苏合丸中起作用的两个成分,其余成分如檀香、青木香、乳香或其油均未能证实其效果。

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