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The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

Pear clone with low-chilling requirement 'SH-085' were planted in containers under shelter, after being grafted on bird pear rootstocks. Two soil water status treatments were undertaken at three different stages after entering short days. Sampling and defoliation were done respectively on Oct. 19, Nov. 3, Nov. 16 and Dec. 1. And then the defoliated plants were treated with high soil water content (-0.01 MPa~-0.05 MPa) to observe the growth and to analyse the accumulation of carbohydrates at various stages.

梨低需冷性新品系'SH-085'嫁接於乌梨砧木上於遮雨的环境下种於栽培槽中，於由长日开始转变为短日后的三个不同时期进行两种不同乾湿程度的水分管理，而后於78年10月19日、11月3日、11月16日及12月1日分别取样及除叶后再以高土壤水分含量(-0.01MPa~-0.05MPa)处理，观察枝梢再生长及开花结果之情形並分析不同时期碳水化合物蓄积之情形。

M/s. The following dimensionless relationships were obtained: Sh=2+0.073Re〓Sc〓〓〓 The desulphurization process by powder injection and blowing in RH refining of molten steel and its mechanism have been considered and analyzed. Based on the tworesistance mass transfer theory and the mass balance of sulphur in the system, a kinetic model for the process has been developed. The related parameters of the model have been reasonably determined. Modeling and calculations for the process by injecting and blowing the lime based powder flux under the assumed operating modes in a RH degasser of 300t capacity have been carried out using the model.

考虑颗粒与液流的相对速度，RH—PTB条件下熔池传质特性可由下式表征： Sh=2+0.073Re〓Sc〓考虑紊流中脉动速度引起的能量耗散，RH—PTB条件下熔池传质过程服从如下关系：〓将粉剂颗粒与钢液间的传质当作刚性的气泡与钢液间的传质处理，对RH—PTB过程有：〓考察和分析了RH喷粉脱硫过程及机理，基于双重阻力传质理论和体系内硫的质量衡算，建立了RH喷粉脱硫过程的动力学模型，确定了模型各有关参数，包括各传质系数和脱硫处理过程中钢液内参与脱硫反应的粉剂量等。

LISTEN SH Super high temperature grease is made from special organic gelatinizer which is added of many anti-oxidation and anti-corrosive additive and super fine soft metal powder.

雷萨SH超级高温润滑脂采用特殊有机稠化剂，稠化高档合成油，并添加抗氧、防腐等多种复合添加剂以及超细软金属粉末制成。

The poly chains can clearly function as steric stabilizers by binding to the gold surface through the thioether but may also (unlike, for example, PEG-SH)4d,h play an electrostatic role due to the potential for this water-soluble ionizable polymer to carry a negative charge.

这个聚甲基丙烯酸链可以很清楚的功能空间稳定器对黄金表面，但也可以通过thioether(不像，例如，PEG-SH)4d，发挥静电作用有潜力的ionizable这类聚合物带负电荷。

This is a natural paper of pure cellulose with a high level of whiteness and wet strength treatment, Sacco embossing and Manter's unique adhesive SH-3020 Plus.

这是纯天然纤维素纸的白度和湿强度的治疗，萨科压花和曼特独特的胶高层次的SH - 3020加。

2B8a was weakly reactive to neutrophils (23.72%) and negative for T cells, NK, DC, RBC and Plt. The antibody reacted to all 3 marrow CD34+ cells with an average positive rate of 39.33% while it was negative for G-CSF-mobilized CD34+ peripheral blood stem/progenitor cells (PBSC, 1.25%). Cell line analysis showed that the antibody notably reacted to three out of 4 cell lines (Raji, SMS-SB, Nalm-6 and Nall-1) with the positive rates of 98.78%, 98.61%, 94.93% respectively and weakly to one of them with 5.68% in B lineage cell lines and monoblastic cell line (U937, 67.78%) while it was only weakly positive or negative for other myeloid leukemia cell lines including Meg01 (33.40%), HL-60 (29.70%),K562 (28.19%), KG1a (16.23 %) and HEL92.1.7 (8.02%). Among 4 T lineage leukemia,5 neuroblastoma and 1 colon cancer cell lines tested, only Molt-3 was found weakly positive (31.40%) for 2B8a, while the remaining 3 T cell lines (Molt4, JM and CCRF-CEM), 5 neuroblastoma cell lines (LA-N1, KCNR, BE, SK-N-SH, SK-N-AS) and the colon cancer cell line (HR8348) tested were negative.

结果表明： 2B8a抗原在外周血B细胞上表达（3/3例，平均阳性细胞数为26.29 ％），而在T淋巴细胞和NK细胞上不表达（0/3例）；在粒细胞和单核细胞上阳性表达均为2/3例，平均阳性细胞数分别是23.72 ％和59.84 ％；在DC细胞、红细胞和血小板上均不表达（0/3例）。2B8a抗原在骨髓CD34＋细胞上的阳性表达是3/3例，平均阳性细胞数39.33 ％，而在G-CSF动员的外周血CD34+细胞上的阳性表达仅1/3例，平均阳性细胞数为1.25 ％。2B8a抗原在B系细胞系Raji、SMS-SB、Nalm-6和Nall-1上的平均阳性细胞数分别为98.78 ％、98.61 ％、94.93 ％和5.68 ％；在T系细胞系Molt-3上的平均阳性细胞数为31.40 ％，而在Molt-4、JM和CCRF-CEM 细胞上不表达；在髓系细胞系U937、Meg-01、HL-60、K562、KG1a和HEL92.1.7上的平均阳性细胞数分别为67.78 ％、33.40 ％、29.70 ％、28.19 ％、16.23 ％和8.02 ％；在神经母细胞瘤细胞系SK-N-SH、KCNR、BE、LAN-1和SK-N-AS细胞以及结肠癌细胞系HR8348细胞上均不表达，而在羊膜细胞系FL细胞上呈一定的阳性表达，平均阳性细胞数为45.03％。

Cell line analysis showed that the antibody notably reacted to three out of 4 cell lines (Raji, SMS-SB, Nalm-6 and Nall-1) with the positive rates of 98.78%, 98.61%, 94.93% respectively and weakly to one of them with 5.68% in B lineage cell lines and monoblastic cell line (U937, 67.78%) while it was only weakly positive or negative for other myeloid leukemia cell lines including Meg01 (33.40%), HL-60 (29.70%),K562 (28.19%), KG1a (16.23 %) and HEL92.1.7 (8.02%). Among 4 T lineage leukemia,5 neuroblastoma and 1 colon cancer cell lines tested, only Molt-3 was found weakly positive (31.40%) for 2B8a, while the remaining 3 T cell lines (Molt4, JM and CCRF-CEM), 5 neuroblastoma cell lines (LA-N1, KCNR, BE, SK-N-SH, SK-N-AS) and the colon cancer cell line (HR8348) tested were negative.

结果表明： 2B8a抗原在外周血B细胞上表达（3/3例，平均阳性细胞数为26.29 ％），而在T淋巴细胞和NK细胞上不表达（0/3例）；在粒细胞和单核细胞上阳性表达均为2/3例，平均阳性细胞数分别是23.72 ％和59.84 ％；在DC细胞、红细胞和血小板上均不表达（0/3例）。2B8a抗原在骨髓CD34＋细胞上的阳性表达是3/3例，平均阳性细胞数39.33 ％，而在G-CSF动员的外周血CD34 细胞上的阳性表达仅1/3例，平均阳性细胞数为1.25 ％。2B8a抗原在B系细胞系Raji、SMS-SB、Nalm-6和Nall-1上的平均阳性细胞数分别为98.78 ％、98.61 ％、94.93 ％和5.68 ％；在T系细胞系Molt-3上的平均阳性细胞数为31.40 ％，而在Molt-4、JM和CCRF-CEM 细胞上不表达；在髓系细胞系U937、Meg-01、HL-60、K562、KG1a和HEL92.1.7上的平均阳性细胞数分别为67.78 ％、33.40 ％、29.70 ％、28.19 ％、16.23 ％和8.02 ％；在神经母细胞瘤细胞系SK-N-SH、KCNR、BE、LAN-1和SK-N-AS细胞以及结肠癌细胞系HR8348细胞上均不表达，而在羊膜细胞系FL细胞上呈一定的阳性表达，平均阳性细胞数为45.03％。

In order to investigate the quantitative structure-retention relationship in gas chromatography, the molecular structure of monosubstituted alkane RX (X=halogen, OH, SH, NH2) is divided into two parts, R and X, to obtain molecular structure parameters, and the retention times in GC for 37 monosubstituted alkanes RX were determined.

将单取代烷烃RX(X=卤素，OH，SH，NH2等)分子结构分为两个区域来提取分子结构参数，从三方面影响因素(烷基R、取代基X、R与X的相互作用)来定量关联RX的气相色谱保留时间。

For propelling the slurry of low density (below 30% in weight) and low anti-corrosion it is an ideal energy-saving product to replace the WARMAN double-suction pump in L and SH double-suction clear water pump series.

输送低浓度(体积浓度20%以下)，低磨蚀渣浆时是替代L系列WARMAN泵和SH型双吸清水泵的理想节能换代产品。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。