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Methods The HPV16E7 gene was cut to three parts, amplified by PCR, and connected individually with BPVL1 on plasmid PUC. With PVL1393 as a transfect vector, the BPVL1/HPV16E7 recombinants were transfected to baculovirus which subsequently expressed the chimeric BPVL1/HPV16E7 protein in the SF-9 cells. The recombinant proteins were then purified by centrifuge, sonication, sucrose and CsCl ultracentrifuge, and were identified by SDS-PAGE, Western blot, ECL and TEM.

HPV16E7基因分3段经PCR扩增后分别克隆入连有BPVL1的质粒PUC形成BPVL1-HPV16E7;以质粒PVL1393为载体将BPVL1-HPV16E7基因转染杆状病毒并在昆虫细胞中进行表达;用超声粉碎和蔗糖超离、氯化铯梯度离心等方法纯化以及免疫印迹、ECL、透射电镜等方法鉴定表达产物。

From January 2007 to December 2007, the samples of this study were the patients who were under 15 years old in alleviated period by purposive sampling. They were divided into two groups:(1) 96 patients participated in the Chinese and western medicine care group;(2) 64 patients participated in the western medicine care group. The SF-36 health questionnaire for pre- and post-tests were administered to all participants. The cost took account of national health insurance apply claims and ratio of hospital's cost and fee.

收案期间为2007年1月1日至12月31日,样本来源分别为中医优质门诊照护计划合作之医疗院所及西医小儿过敏科门诊,采立意取样的方式,年龄为15岁以下由医师确诊为小儿气喘患童,比较其不同治疗模式:接受中西医联合照护组与只接受西医治疗组,在治疗前后测量其生活品质的改变情形,并收集各组健保申报费用及医院成本费用比率资料,最后进行两组成本效果分析比较。

The service you provide is great and it will be a pleasure to do future business with you, said SF of Weyerhaeuser.

您所提供的服务是伟大的,这将是一个令人感到愉快,未来的业务与你说,协的惠好。

Function : The product uses for natural gum and various kinds of rubber alloy , it is responsivity inextractable antiager SF-98. maleimide system can acceptfreeradical to open double linkage during vulcanization.It forms into horizontal linkage to come into being crosslinking with rubber .it has inextractable antiager effect .and horizontal linkage for thermal oxidation has excellent stability.

功用:该品用于天然胶及各种成合胶,是一种反应性不抽出防老剂,由于马来酰亚胺体系在硫化过程中可接受自由基打开双键,形成横键与橡胶产生交联,起到了防老不抽出的作用,同时这横键对于热氧化作用具有很高的稳定性。

It is the first time to clone the BiVP gene from the bumblebee, Bombus ignitus and express it in Spodoptera frugiperda Sf-9 cell. The purified recombinant protein fulfilled the needs of further studying the bioactivity of this protein.

首次在红光熊蜂中克隆到蜂毒蛋白酶基因,在昆虫杆状病毒表达系统中进行了表达,并对表达产物进行了纯化,为深入研究BiVP的生物活性奠定了基础。

Spinrad sees romanticism and science as having been damagingly split during the New Wave vs HARD SF debates of the 1960s; only with cyberpunk, he argues, did they fuse together again.

斯平拉德认为在20世纪60年代的新浪潮与硬科幻的冲撞中,浪漫主义与科学被严重的分离了;只有通过赛博朋克,他说道,它们两者才又融合在了一起。

Spinrad sees romanticism and science as having been damagingly split during the New Wave vs HARD SF debates of the 1960s; only with cyberpunk, he argues, did they fuse together again.

斯平拉德认为20世纪60年代伴随着新浪潮与硬科幻两股思潮的撞击,浪漫主义与科学。。。

The DASI scores showed no difference between the PCI and medical-therapy groups at one year and beyond, although there was an initial advantage for the PCI-treated patients.

PCI组和药物组两年的SF-36数值无临床差异和统计学意义。像心绞痛和呼吸困难等次要终点,PCI组有优势,但在研究结束时两组差异极小。

While the predominant musical force in SF was still dark, dubby House and Wicked–style Breaks, the city embraced the downtempo movement with a healthy bunch of live bands and DJs generating the tunes.

虽然音乐的主要力量科幻仍然黑暗, dubby众议院和邪恶风格的休息,城市拥抱downtempo运动与健康的一堆现场乐队和产生的DJ音乐。

Tudy on the application of the Chinese version of SF 36 scales and selection of interceptive cents for its grade range[J].

F 36量表中文版的应用及分级截断点选择的研究[J]。

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推荐网络例句

Neither the killing of Mr Zarqawi nor any breakthrough on the political front will stop the insurgency and the fratricidal murders in their tracks.

在对危险的南部地区访问时,他斥责什叶派民兵领导人对中央集权的挑衅行为。

In fact,I've got him on the satellite mobile right now.

实际上 我们已接通卫星可视电话了

The enrich the peopling of Deng Xiaoping of century great person thought, it is the main component in system of theory of Deng Xiaoping economy, it is a when our country economy builds basic task important facet.

世纪伟人邓小平的富民思想,是邓小平经济理论体系中的重要组成部分,是我国经济建设根本任务的一个重要方面。