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Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

该论文在综述前人工作的基础上,开展了如下未见文献报道的创新性的研究工作:(1)使用灵敏的衍生试剂对麻黄碱和伪麻黄碱在水体系中的衍生反应条件进行了系统研究,建立了微乳电动色谱-激光诱导荧光检测法和胶束电动色谱—激光诱导荧光检测法测定麻黄碱和伪麻黄碱的灵敏分析新方法,与以前的报道相比,灵敏度和分析时间均有很大改善;(2)使用荧光素钠作为背景荧光试剂,建立了同时分析测定六种黄酮类化合物(秦皮甲素、秦皮乙素、异秦皮定、染料木素、柚皮苷和槐角苷)的毛细管区带电泳—间接激光诱导荧光检测新方法,扩大了激光诱导荧光检测的应用范围,对难衍生化合物的分离分析提供了一种新思路和新途径;(3)以荧光素钠作为背景荧光试剂,建立了一种用于同时测定食品提取DNA中的腺嘌呤和鸟嘌呤含量的胶束电动色谱—间接激光诱导荧光检测新方法;(4)用动态和静态激光散射法研究了模拟生理条件下人血清白蛋白与氨比西林钠盐相互作用所形成的复合物,为药物分子与蛋白质的相互作用、药代动力学等的研究提供了一种新思路。

Objective To study the relationship of serum HGF content and survival rate on squamous cell carcinoma of esophagus patients.

目的 探讨血清中肝细胞生长因子含量对食管鳞状细胞癌的影响。

A Study of the Relationship Between Serum Esterase Polymorphism and Reproductive Performance of Saba Pig.

撒坝猪血清酯酶多态性与繁殖性能关系的研究。

Objective To investigate the relationship between the level of testosterone and estradiol in serum and central serous chorioretinopathy.

目的 探讨血清中睾酮和雌激素水平与中心性浆液性脉络膜视网膜病变发病的关系。

Objective:To investigate the effect of puerarin injection on serum estradiol in female rats with ovary removed.

目的:观察葛根素注射液对去卵巢大鼠血清雌二醇(E2)水平的影响。

Results The level of serum estradiol in the operated groups was significantly decreased.

结果 手术组术后4~8周血清雌二醇的浓度显著低于正常组和假手术组(P.05)。

Objective To explore the relationship between serum estradiol and threatened abortion in women with pregnancy of 4 to 8 weeks.

目的 探讨妊娠4~8周孕妇血清雌二醇(E2)变化及其与先兆流产的关系。

The level of estradiol in serum of the rats were examined by radioimmunoassay.

同时采用放射免疫分析方法检测大鼠血清雌二醇的浓度。

Methods: The serum estrin (E2), progestogen, follicule stimulating hormone and luteotropic hormone were measured in 15 patients with hysterectomy, 22 patients with uterine arterial embolization and 29 normal persons, respectively.

对15例子宫切除患者、22例子宫动脉栓塞患者和29例正常人分别测定血清雌二醇、孕酮、卵泡雌激素和黄体生成素。

The high levels of serum FCH,TG and apoA1,maladjustment of menstruation,usage of estrin,well history of mammary gland disease and history of mammary gland trauma may be the risk factors for occurrence of breast cancer.

结论血清FCH、TG、apoA1水平高,月经不规律,使用雌激素,乳腺良性病史,乳腺外伤史可能为乳腺癌发生的危险因素。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力