查询词典 serum-therapy

Serum samples were then stored in 1.0 ml Eppendorf tube at -10C until assayed.Two hundred u 1 of thawed serum were piped into a 1.0 ml Eppendorf tube.

动物及给药方法体重在230g左右的Sprague Dawley清洁级雌性大鼠60 浙江大学2002年硕士学位论文只。

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

2DRG co-cultured with sciatic nerve undergoing Wallerian degeneration for 14days in vivo in serum free DMEM/F12,1sciatic nerve segment was added into co-culture system at the first moment,the axons were strong and straight and could adhered to each other,and the number of axons dramatically reduced compared with that of DRG cultured alone in serum free medium.2DRG cultured in serum free DMEM/F12for 4days,then the sciatic nerve segment was added into the culture system for 3days,the axons became straight and part of them adhered to each other.

分别在无血清和有血清的条件下将新生鼠的背根神经节和体内变性14天的大鼠坐骨神经段进行联合培养作为实验组，并以在相应条件下单独培养的DRG的轴突的生长情况作为对照组来观察比较轴突的生长情况。

Their hypolipidemic effects were investigated with normaland alloxan induced diabetic mice models. In normal mice group administrating 250mg/Kg/day s. c. injection for 8 days, clofibrate preparations almost significantly decreased serum level of triglyceride; furthermore, administrating 500mg/Kg/dayfor 8 days, whole preparations significantly lowered serum level of triglyceride andpartialy lowered serum level of cholesterol.

以正常及以Alloxan诱发糖尿病雄性小鼠为试验模式，评估制剂的降血脂效果：各制剂皮下注射250mg/kg/day 8日，大都能显著降低正常小鼠的三酸甘油脂的含量；同法给与500mg/kg/day 8日，则全部都有降低三酸甘油脂的含量，且也有部分降低胆固醇的效果。

After the rats were orally administered with 0.066 4 g/, 0.134 g/, 0.4 g/ lycopene and the oil for 30 days, the concents of serum MDA, the energy of serum SA, the energy of serum GSH-Px and the contents of lipofasciin in liver were observed.

各组大鼠分别经口灌胃给予0.066 4 g/？0.134 0g/？0.400 0 g/的番茄红素及食用油30天后，测定血清中MDA含量？超氧化物歧化酶活力？谷胱甘肽过氧化物酶活力？

Compared with these of fat-fed group, the body weight, blood glucose levels after oral glucose and the glucose area under curve, 40min and 90min blood glucose levels after insulin injection subcutaneously and the glucose area under curve in the insulin tolerance test, the fasting serum insulin, insulin resistance index, serum cholesterol, triglyceride and lower density lipoprotein cholesterol decreased, the fasting serum leptin, high density lipoprotein cholesterol and C18:2 were significantly increased in N-6 PUFAs group.

n-6脂肪酸组从第1~10周体重均较高脂组明显减轻，糖负荷后血糖和糖耐量试验中葡萄糖曲线下面积、皮下注射胰岛素后40min、90min血糖及胰岛素耐量试验中葡萄糖曲线下面积、血清胰岛素、胰岛素抵抗指数、血清胆固醇、甘油三酯和低密度脂蛋白含量均较高脂组明显降低；血清瘦素水平、高密度脂蛋白、18碳2烯酸较高脂组明显升高。

Rabbit was immunized by the conjugated alginate-BSA (1.0mg/kg) for 40-days routine immunity method; ELISA method was used to examine the titration of anti-alginate serum; The first generation parotid acinar cells were chosen to divided five groups (group A :contrast, group B: BAS, group C: alginate, group D: anti-alginate serum, group E: alginate +anti-alginate serum), We examined the proliferation of each part by MTT method at four times (lh, 6h,12h and 24h); In the meanwhile, The growth and shape of parotid acinar cells were observed under phase contrast microscope and scanning electron microscope.16 of the immunized rabbits by alginate were divided equally into Al and Bl groups, 12 normal rabbits were divided equally into A2 and B2 groups as control groups, Each rabbit was affused by 0.3ml(40fil/ml)alginate though parotid duct once time two days.

将藻酸盐与BSA偶连形成具有较强免疫原性的Alginate-BSA交联物免疫兔，按抗原量1.0mg/kg接种于新西兰白兔皮内，间隔10d加强免疫1次，共加强4次后，利用间接ELISA法检测藻酸盐抗体效价：将原代培养的兔腮腺腺细胞按10~4个/ml接种到96孔板上，置入细胞培养箱继续培养3d，分为5组：A空白对照组、B牛血清白蛋白组、C藻酸盐组、D抗藻酸盐血清组和E抗藻酸盐血清+藻酸盐组，分别加入PBS、牛血清白蛋白、藻酸盐或新鲜抗藻酸盐血清继续培养，分别于1h、6h、12h和24h不同的时间点，取出96孔培养板，采用MTT法检测5组腮腺腺细胞增殖情况

Methods 34933 serum samples were screened by ELISA method on Treponema pallidum antibody. The samples with probable positive result were retested by toluidine red unheated serum test and Treponema pallidum particle assay. Results There were 559 serum samples with probable positive result.

对34933例标本采用酶联免疫吸附试验进行梅毒螺旋体的初筛，初筛结果为可疑阳性的样本进行梅毒螺旋体明胶凝集试验和甲苯胺红不加热血清反应素试验的检测。

We collected Leishmania promastigotes,washed its with 0.01mol/LpH7.2 buffers ,smashed its,and then measured protein content and titrated work concentration.2.We tested Kala-azar patients"serum and healthy persons"serum of epidemic area by ELISA. The results of three antigensmethods were the same, work concentration of serum was the same(l :100).Results:The protein content of three antigens is, 901 strain with 1000ug/ml,801 strain with 960ug/ml and 951 strain with 1100ug/ml. Suitable work concentration is, 901 strain with 10ug/ml ,801 strain with 12ug/ml and 951 strain with 11ug/ml.

1、抗原的提取：收集新疆不同地区分离得到的利什曼原虫前鞭毛体，经培养，用0.01mol/L pH7.2的磷酸盐缓冲液漂洗，超声粉碎，比色法测定其蛋白质含量，间接ELISA法滴定出抗原的工作稀释度。2、检测：用提取的三株抗原，分别进行间接ELISA法测定黑热病病人血清和疫区健康人血清抗体，并以肺结核病人血清、非疫区健康人血清做对照，三株抗原所用检测方法一致，血清工作稀释度均为1∶100。

Experimental Center of National Level One, Hubei Traditional Chinese Medica l College, Wuhan 430061）Abstract To investigate the protective effects of Jiaweisinisa n on acute liver injury induced by intraperitoneal injection of D-ga lac-tosamise, serum superoxide dismutase, malondialdehyde , glutathioe contents were measured. The pathological changes of liver wer e observed simultaneously. The results showed that JWSNS could decrease serum MD A content, but increase serum SOD and GSH activities in acute liver injury induc ed by D-GalN. It was also found that JWSNS could alleviate the degeneration and necrosis of the hypatocytes.

为研讨加味四逆散防治肝损伤的作用机理，通过动物试验，先用加味四逆散预防给药，再采用D-氨基半乳糖造成大鼠急性肝损伤模型，然后检测大鼠血清超氧化物岐化酶、丙二醛、还原型谷胱甘肽水平，同步观察该方对急性肝损伤大鼠体内脂质过氧化反应及肝脏病理改变的影响，结果表明该方可明显升高大鼠血清SOD、GSH水平，降低MDA水平，肝脏病理学检查亦表明该方使肝细胞变性坏死程度减轻。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。