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The EAE rats were induced by injecting subcutaneously an emulsion of GPSCH and CFA. In addition, each rat received an intracutaneous injection of pertussis. Albumin in serum and CSF samples was examined on the days of 4,6,8,10,12,14,16,18 and 20 p.i.. The integrity of BBB was assessed by caculating CSF to serum albumin quotient. At the same time the brains, spinal cords, thymus and inguinal lymph nodes were removed for paraffin sections cutting. These sections were used for HE staining and ICAM-1 semiquantitative immunohistochemical staining. The results showed as follows:1. None of EAE rats on days 4,6 and 8 p.i. showed clinical signs whichbecame apparent on day 10 p.i.(2.33±0.76; n=6). On days 12 and 14 p.i.

检测p.i。第4天、6天、8天、10天、12天、14天、16天、18天、20天血清和CSF中ALB含量,其CSF/S ALB比值作为评价BBB损害的指标;同时段取动物脑、脊髓、胸腺和腹股沟淋巴结制成石蜡切片行HE染色和ICAM-1半定量免疫组化分析,结果显示如下: 1、EAE大鼠p.i.4-8天无任何临床表现;p.i.10天症状开始明显,症状评分为2.33±0.76(n=6);p.i.12-14天为发病高峰期,症状评分为3.75±0.54(n=12),病情由最初的活动减少,体重减轻逐渐发展为肢体无力、瘫痪,共济失调,甚至濒死状态;p.i.16天大部分动物症状开始缓解,评分为2.08±0.66(n=6);p.i.20天疾病症状完全消失。

Methods Bile acids were continously infused into the Wistar rats to simulate the serum concentration change of patients with obsturctive jaundice.Serum bile acids concentration,arterial blood ketone body ratio and energy charge of liver were determined.

经Wistar大鼠颈内静脉持续给予外源性胆汁酸,升高其外周血清中胆汁酸浓度,模拟阻塞性黄疸患者外周血清中胆汁酸的变化规律,观察腹腔动脉血酮体比值及肝组织能荷变化,时间一周。

It was subsequently realized that the product's bioactivity was dependent upon a critical concentration of three bioactive proteins contained in the milk serum: i.e., the thermolabiles—serum albumin, alpha lactalbumin and lactoferrin.

我们到后来才知道这产品的生理活性是来自乳浆所含的三种具有生理活性蛋白质的浓度,它们是:对温度敏感的血清白蛋白、α乳白蛋白及乳铁蛋白。

During secondary inner ear immune responses against keyhole limpet hemocyanin,to understand the change of interleukin-6 (IL-6) levels in perilymph and serum, IL-6 levels in perilymph and serum were investigated using an immunoassay.

为了解在用keyhole limpet hemocyanin引发的大鼠继发性内耳免疫反应时,实验动物血清及外淋巴液中是否有细胞因子活性的变化,采用免疫测定方法对实验动物血清及外淋巴液中白细胞介素(interleukin-6,IL-6)活性进行测定。

Phenotype analysis of transgenic mice Anti-IgM/anti-IgD antibodies, anti-IgM/anti-IgM〓/anti-IgM〓 antibodies were used to analyze allelic exclusion of transgenic mice B cells, and it was shown that 50%~80% B cells express transgene encoded IgM. ELISA was used to evaluate serum antikeratin IgM and total IgM of transgenic mice peripheral blood, and it is found that sera antikeratin IgM level of several lines of transgenic mice is significantly higher than littermate control. However, there is no difference in serum total IgM level between transgenic mice and non-transgenic mice.

三、抗体重链转基因小鼠表型分析以anti-IgM/anti-IgD抗体、anti-IgM/anti-IgM〓/anti-IgM〓抗体分析转基因小鼠B细胞等位基因排斥情况,发现50%~80%的B细胞表达转基因编码的IgM;ELISA分析转基因小鼠外周血中抗角蛋白IgM和总IgM水平,发现部分转基因小鼠的抗角蛋白IgM水平明显高于对照小鼠,而总IgM水平没有差异。

The RLS method exhibits lower variation in response signal for the same weight of BSA, human serum albumin and γ-globulin and has been successfully applied to the determination of the total protein in human serum samples.

用共振光散射方法对相同量的牛血清蛋白,人血清蛋白和γ—球蛋白进行测定,其相应的信号变化不大。

In this paper, the interaction between luteolin and human serum albumin and bovine serum albumin was studied by using fluorescent spectra and UV absorption spectra.

摘 要:本文通过荧光光谱和紫外吸收光谱的方法,探讨了木犀草素与人血清白蛋白和牛血清白蛋白之间的相互作用。

GanLe capsule can regulate the engulfing index of little mices serum anthracic,can increase the contain of serum hemolysin,and the contractible thymus by CTX,but it have no effect to the T- lymphopoiesis out of body.

肝脏病理检查发现,肝乐胶囊用药组病理损伤较病毒对照组轻。免疫试验结果表明肝乐胶囊能提高小鼠血清碳粒廓清指数(P〈0.05);增加小鼠血清循环抗体的含量(P〈0.05);对抗CTX致小鼠胸腺萎缩的作用(P〈0.001)。

in order to probe into whether glycine supplementation plays an adjusting role in sports performance and immune functions, the authors divided 30 mice randomly into an exhaustive exercising group (e1), glycine supplemented exhaustive exercising group (e2) and a normal resting control group, with 10 mice in each group, supplemented glycine to mice in group e2 and physiological saline to mice in the other two groups, let mice in groups e1 and e2 do an exhaustive swimming exercise 4 weeks later, applied the trace ch50 hemolysis method to analyze supplement activity, applied the mtt method to perform a quantitative analysis on the lymphopoiesis ability of spleen, and thus revealed the following findings: compared with mice in group e1, the time taken by mice in group e2 to swim to an exhausted state is significantly longer (p.05); total supplement activity in blood serum of mice in group e1 is significantly lower than the same of mice in group s (p<0.01), and total supplement activity in blood serum of mice in group e2 is significantly higher than the same of mice in group e1 (p.01), but still significantly lower than the same of mice in group s (p.05); as observed from the test of the lymphopoiesis ability of spleen, the lymphopoiesis ability of spleen of mice in group e1 is significantly lower than the same of mice in group s, and the lymphopoiesis ability of spleen of mice in group e2 is significantly higher than the same of mice in group e1, but still significantly lower than the same of mice in group s.

四型肽精氨酸脱氨酶在类风湿性关节炎。。。以马来酸酐与氨水为原料热缩聚法合成。。。摘要:为探讨甘氨酸补充对运动能力及免疫功能是否具有调节作用,将30只小鼠随机分成力竭游泳运动组(e1)、甘氨酸补充后力竭游泳组(e2)和静息正常对照组,每组10只。在e2组补充甘氨酸,其余两组给予生理盐水,处理4周后,e1组和e2组均做力竭性游泳运动。采用微量ch50溶血法分析补体活性,采用mtt法定量分析脾脏淋巴细胞增殖能力。结果显示,与e1组比较,e2组游泳至力竭的时间显著延长(p<0.01)。e1组血清总补体活性显著低于s组(p<0.01),e2组显著高于e1组(p.01),但仍显著低于s组(p.05)。在脾脏淋巴细胞增殖试验中观察到,e1组显著低于s组,e2组显著高于e1组但仍显著低于s组。

At the same time,the protective effect of mannital and VitC were assessed.Result:The serum level of creative phosphokinase and malon dialdehyde was higher,but superoxide dismutase activity was lower during reperfusion than in the ischemic period.The tissue structure and ultrastructure were damaged more seriously in reperfution period. After treated by mannital and VitC, the level of CPK and MDA in serum was lower, but SOD activity rasied, and the ultrastructure was better.

结果:再灌流组较缺血组血清CPK增高、MDA升高,再灌流组脑组织中MDA 升高,血清中SOD活力下降,再灌流组脑组织结构和超微结构破坏更加严重,应用自由基清除剂后血清中CPK降低,MDA含量降低,SOD活性升高,组织中MDA含量亦降低,超微结构较前好转。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力