查询词典 serum-disease

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低，可能是由于其分子量较小，所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中，利用硫氧还蛋白融合方式对该抗原进行修饰，并在大肠杆菌中表达融合蛋白(Trx-B_2)，纯化该蛋白后免疫小鼠及家兔，结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后，得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度，并从免疫后的兔血清中提取IgG包被酶联板，利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清，结果阳性率分别为87.5%和43.75%，符合率为71.88%。

MethodShengneng reagent and Shenneng control serum Ⅰ,Ⅱ(Lot NO.3229, Lot NO.4339) and patients' serum were used to evaluate the precision, linearity, accuracy and the schlepped polluting rate of 7180 biochemistry autoanalyzer by detecting these items as alanine aminotransferase, total protein, calcium, glucose, creatine Cre） and triglycerides.Hitachi 7600 autoanalyzer was used as the control analyzer to evaluate the correlation.

采用上海申能试剂，申能质控血清Ⅰ、Ⅱ（批号NO.3229，NO.4339）和病人血清，选定包括丙氨酸胺基转移酶、总蛋白、葡萄糖、钙、肌酐、甘油三酯在内的六个项目对7180进行精密度、线性、准确度及携带污染率的评价，并且与日立7600型生化仪进行相关性评价。

MethodShengneng reagent and Shenneng control serum Ⅰ,Ⅱ(Lot NO.3229, Lot NO.4339) and patients' serum were used to evaluate the precision, linearity, accuracy and the schlepped polluting rate of 7180 biochemistry autoanalyzer by detecting these items as alanine aminotransferase, total protein, calcium, glucose, creatine Cre） and triglycerides.

采用上海申能试剂，申能质控血清Ⅰ、Ⅱ（批号NO.3229，NO.4339）和病人血清，选定包括丙氨酸胺基转移酶、总蛋白、葡萄糖、钙、肌酐、甘油三酯在内的六

The results showed that: 1 SOD and GSH-PX activity of hepatopancreas and serum were significantly or extremely significantly increased when 5% or 10% alfalfa meal was added to the diet, whereas the MDA content of the two groups decreased. 2 The concentration of TC and TG in serum, hepatopancrac and muscle dropped as the amount of alfalfa meal increased.

结果显示，1添加5%和10%的苜蓿草粉能显著或极显著提高黄河鲤鱼血清和肝胰脏中超氧化物歧化酶和谷胱甘肽过氧化物酶活力，同时也能显著或极显著降低丙二醛含量，说明苜蓿草粉能有效地提高黄河鲤鱼的抗氧化能力。2血清、肝胰脏和肌肉中总胆固醇和甘油三酯含量都随着苜蓿草粉添加量的增加依次降低。

Experiment 3: By the 30d feeding method of toxicology in vivo test, it was detected that the effect of olaquindox on blood corpuscle, immunity of erythrocyte, antioxidase system activity of blood serum, some biochemistry item of blood serum, histopathology, subcellular structure and deletion of liver mitochondria DNA and so on, Based on Kunming mice divided randomly into 6 groups .

试验三：采用毒理学体内试验30d 喂养法，将小鼠随机分成6组，检测喹乙醇对血细胞、红细胞免疫功能、血清抗氧化酶系活性、血清多种生化指标、组织病理学、亚细胞结构及肝脏线粒体 DNA 缺失等产生的影响。

Swine enteropathogenic E. colis were rejuvenescenced and 6 of them were selected to prepare blood serum. We had tested the reactance of cross agglutination and agar diffusion between the strains and the blood serums. No. 300 and No. 343 were selected because they had highly cross reaction. To test superantigen of No.300 and No.343, the antigens made by both of them immunized piglets, and then we detected the serum 0D values with indirect ELISA. The OMP and PF of No. 300 and No. 343 were extracted for SDS—PAGE and westen-blotting.

本研究针对10株猪致病性大肠杆菌，经复壮后选取致病性强的6菌株作为供试菌株，制备免疫血清与这6菌株的多种抗原分别进行交叉凝集试验和琼脂扩散试验，根据各菌株抗原的交叉反应程度，筛选出抗原交叉程度高的菌株300和343，检测两菌株超抗原的毒性作用，以不同方式制备抗原免疫仔猪，用间接ELISA检测免疫效果，并提取菌株300和343的外膜蛋白和渗透因子经SDS—PAGE及免疫印迹试验，进行相关分析。

After three months, gained blood from cardinal vein and detected the level of serum E2; put all rats to death and got femur bone tissue with asepsis condition. Detected the transcriptional level of PTHR1 gene with RT-PCR. And detected the expression level of PTHR1 protein with Western blot. Results: In ovariectomized groupthe level of serum E2 was 13.80±1.10 pmol/L, the E2 of model control group was 23.51±1.20 pmol/L.

术后3个月腹主静脉取血，测定各组大鼠血清E2水平；分别用小动物骨骼强度测定仪和双能X线骨密度仪检测右股骨生物学力学和骨密度；从左股骨中提取总RAN和总蛋白，分别检测PTHR1在转录和翻译水平上的变化。

Objective To discuss the value of detection of serum serum anti-nuotrophil cytoplasmic autoantibodies and anti-vascular endothelial cell antibodies in the diagnosis of erythema nodosum. Methods Samples from erythema nodosum patients and normal people were collected, the ANCA and AECA were detected by indirect immunofluorescence.

目的 探讨血清抗中性粒细胞胞浆抗体及抗血管内皮细胞抗体在结节性红斑发病中的作用和意义方法收集结节性红斑患者和正常人血清，用间接免疫荧光技术检测血清中ANCA和AECA，并分型。

ConclusionThe positive detect ion rate of serum Cys Cis higher in early stage of diabetic nephropathy group.The serum Cys Chas important diagnostic value in early stage of diabetic nephropathy group.

血清Cys C在早期糖尿病肾病中有较高的检出率，对早期糖尿病肾病的诊断具有重要价值。

Methods With the immunity transmission turbidimetric method, we examined in 178 examples ACS the patient blood serum CRP density, in 50 examples stable angina pectoris the patient, in 66 examples unstable angina pectoris the patient, in 62 examples myocardial infarction the patient with 94 examples healthy collators blood serum, and carriy on the comparison.

方法］采用免疫透射比浊法，检测178例ACS患者血清CRP浓度，其中50例稳定心绞痛患者，66例不稳定心绞痛患者，62例急性心肌梗塞患者；与94例健康对照者血清，并进行比较。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。