查询词典 serum-disease

In this study, isobaric tags for relative and absolute quantification labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS) was employed to compare the differential protein expressions in MCF7 cells upon 17β-oestradiol (E2) stimulation or serum deprivation versus in normal conditions. A total of 576 proteins with the confidential level of ≥95% were identified, 26 proteins among the 576 proteins were found to be differentially expressed after E2 stimulation or serum deprivation, and among them 10 proteins were up-regulated and 16 proteins were down-regulated by more than two folds.

利用四重用于相对和绝对定量的等量异位标签(isobaric tags for relative and absolute quantification， iTRAQ)标记结合二维液相色谱-串联质谱(two-dimensional liquid chromatography-tandem mass spectrometry， 2D-LC-MS/MS)对雌二醇(17β-oestradiol， E2)和血清各自以及共同作用对MCF7乳腺癌细胞内蛋白质表达的影响进行了比较，共鉴定到置信度在95%以上的蛋白质576种，其中各组相对于正常培养组的细胞共找到26种差异在1倍以上的蛋白质，其中10种上调，16种下调。

The testing methods and targets were shown as below:Recorded milk weights from the 7th day postpartum to 15th day postpartum,and calculate the average day-milk yield;Milk samples were draw in 7th day and 14th day postpartum,and testing the milk fat,milk lactose,milk protein,milk Zn and the milk somatic cell counts;Blood samples were adopted at the 7th day postpartum,testing the levels of serum PRL; Blood samples adopted at the 1st day and the 12th day in oestrous cycle were used to test the levels of E_2 and P_4 in serum;In the experimental stage, registering the condition of parturition, the mean birth weight of calves,the first oestrus,the oestrus scycle postpartum and the conception rate of the first service after artificial insemination.

试验方法及所测指标如下：于产后第七天到第十四天记录日产奶量，最后计算每组平均日产奶量；每组随机抽取分娩后第七天、第十四天的奶样，测定乳糖、乳蛋白、乳脂、乳锌含量及乳体细胞数；于产后第七天，随机采取奶牛血样，测定血清中PRL含量；于发情周期的当天和第12天，随机采取血样，测定血清中雌二醇(Estradiol，E2)和孕酮(Progesterone，P)的浓度；记录奶牛的分娩情况、犊牛初生重、产后第一次发情日期、产后发情周期，并采用人工授精技术进行受精，记录第一情期受胎率，最后取平均值，作为衡量奶牛繁殖性能的指标。

Results It showed that the serum active enzymatic of 5′-NT in hepatic and gall diseases,osteal diseases levels were increase, especially the more significant increase of the serum active enzymatic of 5′-NT in level appeared in the patients with hepatic and gall diseases,osteal diseases .

目的 检测各型肝胆疾病、骨骼疾病患者血清5′-核苷酸酶(5′-NT)，谷丙转氨酶和γ-谷氨酰转肽酶，碱性磷酸酶酶活性的变化，观察在肝胆疾病和骨骼疾病中的临床意义，同时与正常健康者作对照。

Results It showed that the serum active enzymatic of 5′-NT in hepatic and gall diseases,osteal diseases levels were increase, especially the more significant increase of the serum active enzymatic of 5′-NT in level appeared in the patients with hepatic and gall diseases,osteal diseases .

结果 发现在肝胆疾病和骨骼疾病时，血清5′-NT酶活性升高，尤其是急性黄疸型肝炎、肝癌、胆道疾病患者血清5′-NT酶活性差异有非常显著性（ P ＜0.01）时，随病情好转，血清5′-NT酶活性有所降低。

Results It showed that the serum active enzymatic of 5′-NT in hepatic and gall diseases,osteal diseases levels were increase, especially the more significant increase of the serum active enzymatic of 5′-NT in level appeared in the patients with hepatic and gall diseases,osteal diseases .

作者：李顺君，黄文方，饶绍琴目的检测各型肝胆疾病、骨骼疾病患者血清5′-核苷酸酶(5′-NT)，谷丙转氨酶和γ-谷氨酰转肽酶，碱性磷酸酶酶活性的变化，观察在肝胆疾病和骨骼疾病中的临床意义，同时与正常健康者作对照。

The results showed that the 56kD and 26kD antigens had better immunogenicity and could not react with the sheep positive serum of Mycoplasma pneumonia and negative serum of E. ovis. Of the 64 samples detected, the positive rate was 66.7%(42/64), 22.9% higher than that of direct microscopic examinerion.

结果表明，56kDa和26kDa的羊附红细胞体抗原具有较好的免疫性，而且不与羊附红细胞体阴性血清和羊支原体病阳性血清发生交叉反应，具有较好的特异性；ELISA检测的64份血清样本的阳性率为66.7%，比涂片镜检高22.9个百分点。

Rabbits were immuned with SW-BSA vaccined twice, the serum antibody titer changes were tested by indirect ELISA assay and attacking toxic with Oxytropis kansuensis (10 g/kg/d) when the antibody titers were in higher. At the same time, the serum biochemical indicators were detected.

SW-BSA白油佐剂疫苗免疫家兔2次，间接ELISA法检测血清抗体效价的变化，抗体效价达到较高后进行甘肃棘豆(10 g/kg/d)攻毒试验，同时检测血清各项生化指标。

Lipid profile of serum, plaque area and matrix metalloproteinase content in the innominate artery were assayed. Results: It was shown that the WEP lowered TC, TG and LDL-C levels in serum, and dereased paque area and MMP content in the innominate artery of mice.

结果 蚤休水提液能显著降低小鼠血清的总胆固醇、总甘油三酯以及低密度脂蛋白胆固醇水平，同时减少无名动脉处的斑块面积，降低斑块中基质金属蛋白酶－1(MMP-1)的含量。

The liver and kidney were partly removed and fixed with 4 % paraform phosphate buffer. The remnant of the liver was stored in liquid nitrogen and -80℃. 4.examination: Serum: ALT, AST, BUN, Scr in serum were measured by biochemical analyses and MDA,SOD by spectrophotometric methods and HA , LN, C-IV by radioimmunological methods.

指标检测：血清：全自动生化分析仪检测血清中谷丙转氨酶、谷草转氨酶、尿素氮、血肌酐的含量，放射免疫的方法检测血清透明质酸、层粘连蛋白、IV型胶原，分光光度计法检测血清中超氧化物歧化酶活性及丙二醛的变化。

Methods: Experimental mice were perfused with different dosages of tetracycline. Eighteen hours later, the serum levels of ALT, AST were measured and the liver tissues were observed by photics microscope to find the relative suitable dosage that can obviously induce acute liver injury. Then the suitable dosage was given in the same way, and the levels of ALT, SOD, MDA, GSH-PS, IL-18 in serum were measured 18, 36, 54, 72 h later respectively. The condition of cell apoptosis was analyzed, and the tissues were observed by photics microscope and electron microscope to find the relative suitable time.

先以不同剂量四环素灌胃，18h后检测小鼠血清ALT、AST水平并进行光镜观察，探索导致明显急性肝损伤的相对合适剂量；再以相对合适剂量四环素灌胃，18、36、54、72h后分别检测小鼠血清ALT、SOD、MDA、GSH-PX、IL-18水平及肝细胞凋亡情况，并进行光镜、电镜观察，探索导致明显急性肝损伤的相对合适时间及可能机制。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。