查询词典 serum-disease

The cells were cultured under the following serum microenvironment. The primary cells of autoserum group were cultured with autoserum, changing the medium with fetal bovine serum after passage. The primary cells of homogeneity foreign serum group were cultured with homogeneity foreign serum, changing the medium with fetal bovine serum after passage. The primary cells of fetal bovine serum group were cultured with fetal bovine serum, and cultured with fetal bovine serum after passage. The primary cells of Dulbecco's modified Eagle's medium group were cultured with serum-free DMEM, changing the medium with fetal bovine serum after passage.

分别以下列血清微环境培养：自身血清组：分离细胞后原代用含大鼠自身血清的培养基培养，传代后换用含胎牛血清的培养基培养；同种异体血清组：分离细胞后原代用含同种异体血清的培养基培养，以后用胎牛血清培养基培养；胎牛血清组：分离后用含胎牛血清培养基培养，以后一直用含胎牛血清培养基培养；DMEM组：分离细胞后原代用不含血清的DMEM培养基培养，传代后换用含胎牛血清的培养基培养。

A method of screening serum of patients of gout and hyperuricemia includes checking purine compound content of xanthine and hypoxanthine in serum to be tested, confirming that serum to be tested is serum of gout patient if xanthine content of 1.00-2.35 mg / L and / or hypoxanthine content of 4.1-16.00 mg / L are contained in serum to be tested and confirming that serum to be tested is serum of hyperuricemia patient if xanthine content of 0.20-1.20mg/L and / or hypoxanthine content of 1.30-3.50mg/L are contained in serum to be tested.

本发明所提供的筛查痛风疑似患者及高尿酸血症疑似患者血清的方法，是检测待测血清中包括黄嘌呤和次黄嘌呤在内的嘌呤化合物的含量，如待测血清中黄嘌呤含量为1.00-2.35mg/L，和/或次黄嘌呤含量为4.1-16.00mg/L，则待测血清为痛风疑似患者血清；如待测血清中黄嘌呤含量为0.20-1.20mg/L，和/或次黄嘌呤含量为1.30-3.50mg/L，则待测血清为高尿酸血症疑似患者血清。

The BMSCs were divided into six groups after repeatedly passaged: A,the BMSCs were cultured with conventional culture fluid(DMEM culture fluid+20%fetal bovine serum+2 mmol/L aminoglutaric acid amine) all the time;B,the BMSCs were cultured with conventional culture fluid+HGF(25ng/ml)+dexamethasone10~(-7M;C(HGF and Zuoguiwan induced group), the BMSCs were cultured with conventional culture fluid+ HGF(25ng/ml)+ dexamethasone10~(-7M+ 10%Zuoguiwan drug serum;D(conditioned medium and contrast serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % normal rat serum;E(conditioned medium and Bazhentang drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10 % Bazhentang drug serum;F(conditioned medium and Zuoguiwan drug serum induced group), the BMSCs were cultured with conventional culture fluid+50 % conditioned medium+10% Zuoguiwan drug serum.

常规培养组始终使用常规培养液(DMEM培养液+体积分数20％胎牛血清+2mmol／L谷氨酸胺)进行培养；HGF诱导组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7M进行培养；HGF加左归丸组以常规培养液+促肝细胞生长因子(HGF，25ng／ml)和地塞米松10~(-7／M+10％的左归丸含药血清进行培养；条件培养液加对照血清组以常规培养液+50％的条件培养液+10％正常大鼠血清进行培养；条件培养液加八珍汤组以常规培养液+50％的条件培养液+10％八珍汤含药血清进行培养；条件培养液加左归丸组以常规培养液+50％的条件培养液+10％左归丸含药血清进行培养。

Results l.Serum concentrations of VCAM-1 in PIH (93.39 + 57.3)ug/ml were higher than that in normal pregnant group. Serum concentrations of VCAM-1 in moderate(97.89 + 34.07)ug/ml and severe PIH(132.24 + 60.97)ug/ml were significantly higher than that in normal group.(P.05, P.01). There was no difference between serum levels of VCAM-1 mild PIH and normal pregnant group. 2.Serum levels of IL-6 in PIH group(102.17 ?48. 31)pg/ml were significantly higher than that in normal group(49.16 + 12.9)pg/ml.Serum levels of IL-6 in moderate(95.79+31.19)pg/ml and severe PIH( 127.27+11.3 8)pg,ml were significantly higher than that in normal group. There was no difference between serum levels of PIH in mild PIH (52.13 + 12.90)pg/ml and normal pregnant group. 3. In PIH group, serum concentrations of VCAM-1 correlate with the levels of IL-6,r=0.63. 4.The expression of VCAM-1 in cytotrophoblast of spiral arteries in normal pregnant group(100%) were significantly higher than that in PIH group.Theexpression of VCAM-1 in moderate PIH(37.50%) and sever PIH were lower than that in normal group.There was no difference between the mild PIH and normal group.Conclusions The increased levels of serum of VCAM-1 may participate in the process of vascular endothelium damages in PIH.

结果 1、妊高征组血清VCAM-1浓度为(93.39±57.3)μg／ml明显高于正常妊娠组(44.87±15.60)μg／ml，差别有显著性(P＜0.05)；中、重度妊高征组VCAM-1浓度分别为(97.89±34.07)μg／ml和(132.24±60.97)μg／ml，与正常妊娠组比较，差异有显著性(p＜0.05)和非常显著性(P＜0.01)；轻度妊高征组VCAH-1为(48.46±15.60)μg／ml与正常妊娠组比较，差异无显著性(P＞0.05)。2、妊高征组血清IL-6含量为(102.17±48.31)pg／ml，明显高于正常妊娠组(49.16+12.9)pg／ml，差异有非常显著性(P＜0.01)；中、重度妊高征组IL-6含量分别(95.79±31.19)pg／ml和(127.27±11.38)pg／ml，与正常妊娠组比较，差异有显著性(P＜0.05)和非常显著性(P＜0.01)；轻度妊高征组IL-6含量为(52.13±12.90)pg／ml与正常妊娠组比较，差异无显著性(P＞0.05)。3、VCAM-1与IL-6水平呈明显正相关，r=0.63(P＜0.01)。4、子宫胎盘床螺旋动脉滋养细胞VCAM-1表达，正常妊娠组都存在阳性表达(阳性表达率100％)，妊高征组有10例阳性表达阳性表达率为叩们，差别有非常显著性河＜0.01L 中、重度妊高征组阳性表达率分别为37、50％和0，与正常妊娠组比较，差别有显著性河＜0.05）和非常显著性汀＜0.01太轻度妊高征组阳性表达率为83.33凡与正常妊娠组差别无显著性问＞0.05）。

Results:There were no abdominal pain and fever,jaundice faded away gradually postoperation.Biliary drainage was 300ml-1000ml/d more or less.Colour of bile changed into normal by degrees.Intra peritoneal drainage was open and the colour was light bloody.Amount of drainage dropped off day after day.Whole blood cell test, serum glutamic pyruvic transaminase,serum glutamic oxalacetic transarninase,total serum protein,serum albumin and serum globulin returned to normal in first week postoperation.Serum bilirubin,serum glutamyltranspetidase and serum alkaline phosphatase declined gradually in first week postoperation.T-tube was clipped at twelfth day postoperation.T-tube Cholangiography was clear at 24~ day postoperation.

结果：术后无上腹部绞痛、高热，黄疸逐渐减退，每日引流胆汁300ml-1000ml不等，由淡黄色混浊、内有胆泥过渡到金黄色清亮的正常胆汁，腹腔引流通畅，引流液为淡红色，量从最初的200ml迅速过渡到10ml，血常规、血清谷丙转氨酶、谷草转氨酶、总蛋白、白蛋白、球蛋白等1周内均恢复正常，血清总胆红素、直接胆红素、间接胆红素、谷氨酰转肽酶、碱性磷酸酶逐渐下降，术后12d夹闭T管，术后24d T管造影提示左、右肝管及胆总管下端通畅，无狭窄。

The T; LH and FSH level of serum of male schizophrenia were lower than that of normal control, but the PRL level of serum of male were significant higher than that of normal control, f There wsa a significant relationship between psychiatric symptoms and sex hormones level. There was a positive correlation between extreme and pugnacity factor of PANSS and T level of serum in male schizophrenic patients, There was a negative correlation between psychopathological scores and 〓level of serum in female schizophrenic patients, g There was a significant correlation between neuropsychological tests scores and sex hormones level of serum. There was a significant positive correlation between cognitive function and T level of serum in male schizophrenic patients. There was a significant positive correlation between cognitive function and 〓 in female schizophrenic patients.

治疗后病人的PRL较治疗前有显著提高；6）精神分裂症病人的精神症状与其性激素水平的相关有统计学意义，其中男性病人的T与PANSS中的偏激/好斗呈正相关，女性病人的〓与PANSS中的一般精神病理量表分呈负相关；7）精神分裂症病人的认知功能与其性激素水平的相关有统计学意义，男性病人的神经心理测验成绩与T呈正相关，女性病人的神经心理测验成绩与〓呈正相关。

The results showed that the rates of mature oocyte were 75.2％, 73.1％, 69.8％, 63.5％and oocyte at telophase of MI were 16.3％, 15.9％, 16.9％, 27.0％, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P＜0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P＜0.05); Significant higher rates (66.6％, 66.6％, 73.6％) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3％)(P＜0.05). The rates of oocyte with cortical granules in cortex were 58.8％, 33.9％, 54.7％and 47.9％respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P＜0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三、EGF和TGFα对卵母细胞成熟的影响未成熟卵母细胞分别在TCM199基础培养液+血清、TCM199基础培养液+BSA+EGF、TCM199基础培养液+BSA+TGFα和TCM199基础培养液+BSA四组成熟培养系统中成熟培养，22小时后上述各组卵母细胞的核成熟率分别为75.2％、73.1％、69.8％、63.5％，处于第一次减数分裂末期的比率分别为16.3％、15.9％、16.9％、27.0％，在添加血清、EGF、TGFα各组中核成熟率显著高于其它各组(P＜0.05)，处于末期的比例明显低于其它各组(P＜0.05)：添加血清、EGF、TGFα各组在a-Tubulin蛋白的分布与表达上(66.6％、66.6％、73.6％)明显高于BSA组的正常率43.3％(P＜0.05)；各组CG发生迁移较好的卵母细胞的比率分别为58.8％、33.9％、54.7％、47.9％，血清组和添加EGF组CG迁移至皮质区的比例明显高于仅添加BSA处理组(P＜0.05)。

Sum up as follows now. This group uses 1 clinical data ancon vein is pressed determine heart function of the patient 44, male 24, female 20; age 46 years old of ~79 year old, average 56 years old of; classification by function of new York heart, heart failure Ⅲ spends 6,Ⅱ spends 23,Ⅰ spends 15; right heart failure 31, complete heart failure heart disease of sex of source of 13; lung 14, hypertensive heart disease 8, coronary heart disease 5 (among them acute cardiac muscle straightens dead 2), outspread sex cardiac muscle is ill 7, rheumatism sex heart disease 4, hypertensive heart disease is amalgamative coronary heart disease 3, be short of disease of cardiac muscle of courage and uprightness 2, congenital heart disease 1. 2 detect 2.1 equipment treat the method dish 1, l measure press glass to be in charge of 1 (long 30cm, internal diameter 4mm), also can use measure a head to press a canal to replace, 10mL injector 1, 12500U heparin fluid 1. 2.2 operations patient is taken smooth lie, make be exhibited outside the upper arm show ° of 45 °~60 with trunk, and difficulty breathes like the patient in same horizontal; with right atrium, desirable lie partly, arm height is equivalent to the 2nd costal region.

现总结如下。1临床资料本组采用肘静脉压测定病人的心功能44例，男24例，女20例；年龄46岁~79岁，平均56岁；按纽约心功能分级，心力衰竭Ⅲ度6例，Ⅱ度23例，Ⅰ度15例；右心力衰竭31例，全心力衰竭13例；肺源性心脏病14例，高血压心脏病8例，冠心病5例(其中急性心肌梗死2例)，扩张性心肌病7例，风湿性心脏病4例，高血压心脏病合并冠心病3例，缺血性心肌病2例，先天性心脏病1例。2检测方法2.1设备治疗盘1个，L型测压玻璃管1个(长30cm，内径4mm)，也可以用测脑压管代替，10mL注射器1具，12500U肝素液1支。2.2操作病人取平卧位，使上臂外展和躯干呈45°~60°，且和右心房在同一水平面；如病人呼吸困难，可取半卧位，手臂高度相当于第2肋间。

The levels of schistosome circulating cathodic antigen in serum and urine were detected parallelly by McAb-Dot-ELISA. In 81 acute cases, the positive rates in serum and urine were 96.30% and 66.67%(P＜0.01) but the combined positive (positive in serum and／or in urine) rate reached 100%, while in 109 chronic cases, they were 77.06% and 10.09%(P＜0.01) but the combined positive rate was not increased. In 100 healthy individuals from non-endemic areas, no positives were detected in serum and urine with false positive rate of 0%. The cross reaction rates in serum and urine of 51 cases with clonorchiasis were 9.43% and 0% but all were 0% in 48 cases with ancylostomiasis.

提要 应用McAb-Dot-ELISA平行检测血吸虫病人的血清和尿样(浓缩20倍)中日本血吸虫肠相关趋阴极循环抗原。81例急性血吸虫病患者的血清和尿样的阳性率分别为96.30%和66.67%，而血清和尿样组合检测的阳性检出率提高至100%。109例慢性血吸虫病患者的血清和尿样的阳性检出率分别为77.06%和10.09%，组合检测的阳性检出率没有提高。100份健康人的血清和尿样的假阳性率均为0%。54例华支睾吸虫病患者的血清和尿样的交叉反应率分别为9.43%和0%。48例钩虫病患者的血清和尿样的交叉反应率均为0%。

Preparation for drug serum: 20 Wistars mouses were at random divided intofour group that were fed with regular dosage baoganning (wascomposed of Milkvetch Root, Peach Seed, Baical Skullcap Root, WhitebackleafMallotus Root, Turtle Shell, Dan-Shen Root, RadixBupleuri, White Peony Root,Immature Bitter Orange,1.77g/ml),double dosage baoganning(3.54g/ml),Colchicine(0.05g/ml) and saline for seven days respectively. They were normalmouse serum,serum of baoganning regular dosage, serum of baoganning doubledosage and serum of Colchicine.

研究方法一、药物血清的制备：给正常20只Wistar大鼠随机分组，每组5只，采用血清药理学方法，分组给予保肝宁(由黄芪、桃仁、丹参、黄芩、鳖甲、柴胡、白芍、枳实等组成)等剂量煎剂(1.77g／ml)，保肝宁二倍剂量组煎剂(3.54g／ml)，秋水仙碱(0.05g／ml)灌胃7天，正常对照组大鼠给予生理盐水，常规方法制备含药血清及正常大鼠血清。

As of Tuesday, Google's results were still censored in China.

截至周二，谷歌的搜索结果仍受中国审查。

In order to make the positive action increase and negative one decrease, the sub-forces of the social factors must be adjusted to form a centripetal force.

在这一过程中，人的主体性发挥是社会有机体健康发展的灵魂。