查询词典 sequencing

The answer was that the former could be far sensitive than the latter, but the former needed complex instruments. The latter could help us to directly judge the order of severity by colors.On the other hand, we took advantage of polymerase chain reactionto amplify the MCP genome of the virus and compare it with other nine published MCP genomes of viruses including CzIV, TIV, CIV, LCDV-1, LCDV-K1, BIV, FV3, GIV, RSIV. The sequencing data showed that the MCP of Cobia iridoviruswas more homologous to the MCP of LCDV-1（89﹪similarity）than that from FV3（47﹪similarity）.

另一方面，为了了解引起海鲡病变之病毒究竟属於何种病毒，我们透过聚合酶链锁反应增幅其主要鞘蛋白基因并加以定序，与其他九种已发表的虹彩病毒之 MCP 核酸及胺基酸序列，包括：CzIV、TIV、CIV、LCDV-1、LCDV-K1、BIV、FV3、GIV、RSIV 之 MCP 进行分析比对，显示 Cobia iridovirus亲缘性和感染大多数硬骨鱼类的 Lymphocystivirus 属的 LCDV-1 最为接近（89﹪相似度），和感染两栖类的 Ranavirus 属的 FV3 亲缘性较低（47﹪相似度）。

We identified the likely disease-causing mutation by applying genomic methodologies (multiplex target amplification and massively parallel sequencing) to historical specimens from the Romanov branch of the royal family.

为了弄清该疾病的确切性质，研究人员从最近发现的俄国Romanov家族的遗骸中提取了已经降解的DNA样本，其中包括维多利亚的患有血友病的曾孙Alexei王储的DNA样本。

We identified the likely disease-causing mutation by applying genomic methodologies (multiplex target amplification and massively parallel sequencing) to historical specimens from the Romanov branch of the royal family.

我们从皇室家族Romanov一支取得样本，通过遗传学方法（多元靶点扩增和大量平行测序）确定了可能的致病性突变。

In this study, 2 individuals carrying RHD positive, D antigen negative allele (with exon 1 and 10 of RHD) and their mRNA were investigated by using reverse transcriptase PCR and sequencing through one pair of specific primers for 5′- and 3′-non-coding region of RHD, taking a Rh-positive sample as control.

本文分别针对RHD基因mRNA的5′-和3′-非编码区设计一对特异性引物，通过逆转录PCR和cDNA测序，分析2例RHD基因阳性（拥有第1和第10外显子）、D抗原表型阴性个体的全长mRNA/cDNA序列，同时以1例正常Rh阳性个体作对照。

The sensibility and specificity of the method were evaluated with the amplification and by sequencing randomized Rh-negative and Rh- positive controls.

序列特异性引物方法扩增RHD 基因特异的外显子及其侧翼序列，PCR产物直接序列分析测定核苷酸的变异。

The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.

因胸膜肺炎放线杆菌的致病性主要是由毒素决定的，故参照猪胸膜肺炎放线杆血清2型菌株的序列(GenBankL1 2 1 4 5)设计了一对特异性引物，用PCR的方法扩增apxⅢA基因并得到了长 3 466bp的片段，然后将其克隆到pMD 1 8T中，经酶切鉴定和序列分析表明克隆是成功的；再将apxⅢA插入到原核表达载体pET 2 8b后，转化BL2 1 (DE3)，在IPTG诱导下获得高效表达，经Westernblotting检测证实表达产物有活性。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。