查询词典 sequencing

Results Identification by enzyme restrictions and PCR amplifications, as well as DNA sequencing confirmed that the plasmids were successfully constructed.

结果 限制性酶切、PCR扩增和DNA测序证明质粒构建成功。

It is important to use direct DNA sequencing for determining the mutations or polymorphisms of WD gene.

exon8为我国WD病人基因突变的热区之一，这对于建立准确快速的WD直接基因诊断方法具有重要意义，该测序方法对明确基因变异的具体部位和内容的具有一定的重要性。

Virus isolates have been sent to the WHO Collaborating Centers for Reference and Research on Influenza for sequencing and antigenic makeup characterising to update the diagnostic reagents available for National Influenza Centers .

分离出的病毒已送往世界卫生组织流感确认及研究协作中心进行基因序列和抗原组成特性检测，以更新国家流感中心的诊断试剂。

The whole system, which is powered by a single 5V power, supplies steady core voltage (1.8V) and I/O voltage (3.3V) with high-efficiency synchronous step-down dc/dc converter TPS54314 and TPS54316, presents a power supply sequencing solution for dual supply voltage DSPs and includes a supervisor on the power supplies and reset circuit in order to make the system more stable and efficient.

整个供电系统采用单一的5V电源供电，用高效率的同步电压转换芯片TPS54314和TPS54316芯片分别产生TMS320C6711B DSPs的内核电压（1.8V）和周边I/O接口电压(3.3V)，同时巧妙地解决了两种供电的顺序问题，并增设了电源监控和复位电路，确保供电系统稳定、高效地工作。

The availibility of the extracted TTV-DNA by the two methods, primers and PCR system was testified by cloning and sequencing the 3' and 5'NCR of patient 72#, who was TTV PCR- positive when used the DNA extracted by STG method. The sequence of 3' and 5'NCR showed 88% of analogy to that of Japanese isolate in the GenBank.

同时，说明用STG法提取的TTV DNA、设计的引物和所用的PCR系统可以有效地用于检测TTV，为今后利用PCR研究TTV的致病性等提供了条件。

Environmental bacteria ; Genetic diversity ; 16S ribosomal RNA gene ; Gene tag serial sequencing

环境细菌；遗传多样性； 16S核糖体RNA基因；基因标签串测序法

Dendritic cells are the most potent antigen-presenting cells in human immune system. Our institute has been working on the region of DC development and DC-mediated immune therapy on cancers for a long time. A cDNA library of human dendritic cells has been set up and by large-scared random sequencing, we have isolated a lot of novel fulllength cDNA clones.

树突状细胞(Dendritic cells，DC)是人体内重要的抗原提呈细胞，本研究所对DC的分化发育及其介导的肿瘤免疫治疗的研究工作已进行多年，并在此基础上建立了人树突状细胞基因文库，通过大规模随机测序的方法开展了从DC中克隆新分子的工作，从2万余条EST中发现了许多新的分子。

Finally, the constructed β3AR gene antisense expression vector was identified though restriction endonuclease analysis and sequencing. RESULTS: Target gene of about 1.2 kb was successfully cloned into retroviral vector pLNCX. The sequence of cloned β3AR fulllength cDNA was identical to the reported one in GenBank.

结果：经琼脂糖凝胶鉴定，所克隆的目的基因大约为1.2 kb，并成功地连接到逆转录病毒载体pLNCX上，经测序证明，插入的目的片段其碱基序列与Genbank上报道的完全一致，且插入载体的方向完全正确。

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

Panels of neutralising antibodies are being developed for each serotype and characterised by nucleotide sequencing of escape mutant virus.

针对每一个血清型中和抗体反应板已经开发出来，加入并据核苷酸序列检定免疫逃脱型变异毒种。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。