查询词典 sequencing

Methods The hairpin sequences of siRNAs targeting VR1 gene of rat were designed, and two pairs of oligonucleotide sequence were synthesized. The annealed oligonucleotide fragments were cloned into linearized pRNAT-U6.2/Lenti expression vector and identified by PCR and DNA sequencing.

设计靶向大鼠VR1基因的发夹状siRNA，合成两对互补的寡核苷酸序列，退火后克隆到酶切的pRNAT-U6.2/Lenti siRNA载体，通过PCR和DNA测序鉴定重组质粒。

Results DNA sequencing showed that the oligonucleotide fragments were correctly cloned into linearized pRNAT-U6.2/Lenti expression vector and the expression of VR1 mRNA in L4-L6 DRG neurons was inhibited significantly by pRNAT-U6.2/Lenti-siVR1 after intrathecal injection in rats.

结果 测序鉴定证实目的寡核苷酸片段被克隆到pRNAT-U6.2/Lenti载体中；经蛛网膜下腔注射后，pRNAT-U6.2/Lenti-siVR1可下调正常大鼠L4-L6DRG神经元内VR1mRNA的表达。

Great, realistic, Scrabble-style action Letter bag, personal tray, password protected Automatic turn sequencing and email updates Unlimited simultaneous games Online rule book Winner announcements Administrative controls such as game deletion, score adjustment, and more.

很强的现实针对性，拼字式行动的信袋，个人进纸匣，密码保护，自动转测序和电子邮件更新无限的同时线上游戏规则书赢家公布的行政管制，例如游戏删除，分数调整，更多。

Using the total RNA of merozoites of Eimeria acervulina isolated from Guangdong Pro- vince as template,a partial segment of 3-1E gene was amplified by RT-PCR. The gene fragment 682 bp inlength was cloned into pGEM-T Easy vector,and the recombinant plasmid was identified by PCR,restric-tion enzyme analysis and sequencing. The homology analysis revealed that the nucleotide homology be-tween the 3-1E gene of the E.

根据GenBank中登录的堆型艾美球虫3-1E基因序列，设计了3条引物，以广东株堆型艾美球虫裂殖子总RNA为模板，利用反转录-聚合酶链反应扩增获得了3-1E基因部分片段，将这一片段克隆至pGEM-T Easy载体中，经PCR、限制性内切酶分析和克隆片段的序列测定、比较，证实了克隆片段的可靠性。

Branches, leaves and phloem of apple collected from an old orchard in Yanji, Xinjiang were used for total RNA isolation specific fragments of Apple fruit crinkle viroid were amplified by RT-PCR using the primers disigned according to the sequence AB104558 on GenBank. Ten sequences were obtained through recovering, cloning and sequencing, and also registered in GenBank accession No.

采集新疆焉耆地区的老果园中的苹果树枝条、叶片和韧皮部，提取总RNA，根据GenBank中的AB104558序列设计引物，利用RT-PCR技术扩增出苹果皱果类病毒（Apple fruit crinkle viroid ，AFCVd）特异条带，通过回收、克隆和测序，结果表明，获得的10条序列，已在GenBank中登录（登录号：EU031507～EU031516）。

Compared with low resolution results of HLA-DRB1 alleles,high resolution results were analyzed to see any correlation between HLA-DRB1 alleles and GVHD. by double-blind statistically analysis of HLA-DRB1 high resolution in 48 donor/recipient typings in UCBT.

利用盐析或柱提法提取48例非亲缘性脐血及移植患者全血DNA，采用HLA-SBT方法分别对HLA-DRB1等位基因进行高分辨分型，并与HLA-SSP（HLA-sequencing specific primers）低分辨结果进行比较分析。

Long-PCR amplification, clone and primer-walking sequencing methods were employed in determine the complete sequence of mitochondrial genome of tokay.

还采用长PCR扩增等方法，测定了大壁虎线粒体基因组全序列。

Polymorphism of HLA-DQB1 promoter region in Hans IDDM patients and normal controls have been identified by PCR, PCR/SSCP and PCR/sequencing methods.No differences were found in y and s box between patients and controls carrying different allele as well as in different ethnic groups. There are two different sequences in x box,but CCTAGAGACAGATT sequence locates frequently on the haplotype with DQB1.0302 allele. Polymorphism between transcription point and y box (at position －44～-46 and －59～-61) might be associated with the genetic susceptibility to IDDM. Additionally,a new single base mutant (CACC→CAC A ) was found at position －131 and －128 in two patients carrying DQB1.0601 allele.

结果显示携带不同等位基因的患者与对照者DQB1 5'-调控区y、s box核苷酸序列相同，且与白种人基因结构一致；y box核苷酸序列存在二种结构，CCTAGAGACAGATT序列常常与DQB1.0302等位基因在同一单倍型；转录起始位点至y box间-44至-61位存在多态性，-59至-61位AAG等位基因可能与1-型糖尿病易感相关联；在2例携带DQB1.0601等位基因患者的-131至-128位间发现CACC→ACA A单个碱基取代突变。

To investigate the methods to effectively and simply assess the CAG repeat size of HD gene which was necessary for gene diagnosis of Huntington disease, the sequence including polymorphic CAG repeat of HD gene was amplified by PCR with TaKaRa LA Taq DNA polymerase and GC buffer. PCR products were analyzed on polyacrylamide gel to distinguish normal alleles from HD alleles. The DNA fragments of affected alleles were recovered from polyacrylamide gel as templets for secondary PCR. The secondary PCR products were cloned into T vector for sequencing analysis to determine CAG repeat size. A total of 20 normal individuals and 3 members from a HD pedigree were included in this study.

为了简单高效检测HD基因开放阅读框5'端n三核苷酸重复序列，建立快速准确的亨廷顿病(Huntington disease， HD)基因诊断方法，应用TaKaRa LA Taq DNA聚合酶配合GC buffer扩增HD基因包含n重复序列的目的片段，非变性聚丙烯酰胺凝胶电泳检测后回收n拷贝数异常增多的目的片段，再次PCR扩增后将产物连接至T载体，进行DNA测序确定CAG的拷贝数。

What is making the Human Microbiome Project feasible is the recent development of superfast gene sequencing technologies.

近来基因序列技术的超速发展使人类微生物组计划成为可能。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。