查询词典 sequencing

Methods:DNA was extracted from tissues of HCC and amplified by PCR.MI was determined by using seven microsatellite markers and mutational ana lyses of IGFⅡR by running sequencing gel electrophoresis.

用QIAamp方法提取肝细胞癌DNA，选择7对引物，经PCR扩增，走DNA序列胶电泳，测定其MI的阳性率及有无IGFⅡR基因的突变。

A set of microRNA markers, which is hard to be quantified and quantitated before, in the serum of diabetes patients was also established employing the Solexa sequencing.

这项工作一方面在运用新测序技术研究复杂疾病进行了方法学的尝试，同时，研究结果也丰富了中国人群中II型糖尿病风险基因的研究结果。

This review focuses on newly developed strategies for miRNA quantification, and elucidates in detail the probe-hybridization based methods including Northern blotting, microarray, gold nanoparticle labelling, and splinted ligation with radioactive labels. The amplification-based methods including quantitative PCR, rolling cycle amplification, invader assay, and the next generation sequencing methods were also discussed. The advantages and disadvantages of these methods were compared.

文章系统地介绍了最新发展的microRNA定量检测方法，详细阐述了基于探针杂交技术的Northern blotting法、微阵列芯片法、纳米金标记法、桥连同位素标记法，以及基于扩增技术的定量PCR检测法、滚环扩增法、引物入侵法和新一代大规模高通量测序法等，并对这些方法的优缺点进行了分析比较。

Supplanting this approach with routine sequencing of all the genes is consequently attractive and, more importantly, scalable.

因此，用对所有基因进行常规测序的方法代替当前方法很有诱惑力，并且是可以实现的。

Using a well established model, we systematically analyze fundamental limitations on the viability of using mechanical unzipping of DNA as a fast and inexpensive sequencing method.

我们使用一个好的确定的模型，系统的分析了使用DNA的机械解链作为快速、便宜的测序方法的可行性的重要局限。

Writing in the journal Science, the researchers said they reconstructed the ant family tree using DNA sequencing of six genes from 139 ant genera, encompassing 19 of 20 ant subfamilies around the world.

研究人员对包括全球20个蚂蚁亚群中的19个亚群－139种蚂蚁，用6个基因的 DNA 排序技术重建了蚂蚁家族。

Methods:Direct sequencing of PCR products was applied to study G3316A mutation in blood corpuscle mtDNA in 315 T2DM patients without relation of consenouinity and 158 normal controls from Zhejiang province. The mutation was analyzed with DNASTAR and Antheprot 5.0 softwares.

采用PCR产物直接测序法对浙江籍汉族人群中无血缘关系的315名T2DM患者及158名正常对照的外周血mtDNA进行检测，并用DNASTAR和Antheprot 5.0软件分析突变位点。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

Preparation of quasi-interpenetrating network/functionalized gold nanoparticle composite matrix and its performance for DNA sequencingA new matrix additive,PDMA-functionalized gold nanoparticle, was prepared by "grafting-to" approach to avoid GNP aggregation at high concentration of salt in buffer solution,and then incorporated into quasi-IPN composed of LPA(3.3 MDa) and PDMA to form novel polymer/metal composite sieving matrix(quasi-IPN/GNP-PDMA) for DNA sequencing by CE.

准互穿聚合物网络/官能化的金纳米粒子复合介质的制备及其用于DNA测序性能的研究为了解决GNPs在高盐浓度下易附聚的问题，由&grafting-to&法制备了一种新型介质添加剂，即PDMA官能化的GNPs，并将其加入到由LPA(3.3 MDa)和PDMA所组成的quasi-IPN中得到了用于毛细管电泳DNA测序的聚合物/金属复合筛分介质(quasi-IPN/GNP-PDMA)。

Selecting the better amplified from Diacylglycerol acyltransferase, gene, Acyl- desaturase gene ,then cloning sequencing, afer that, through the NCBI and nucleotide sequence to match homology , the results show that: cloning by the cassava, castor-oil plant Jatropha curcas DGAT and SAD genes and gene fragments have the high homology with other known plant DGAT genes and SAD genes .

从中筛选出扩增效果较好的二酰基甘油酰基转移酶(Diacylglycerol acyltransferase， DGAT)基因，硬脂酰-酰基载体蛋白脱饱和酶(Acyl- desaturase， SAD)基因克隆测序，经测序，通过NCBI与已知的核苷酸序列进行同源性比对，结果表明：克隆所获得的木薯、蓖麻和麻疯树 DGAT基因和SAD基因的片段与其它已知植物的DGAT基因和SAD基因具有很高的同源性。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。