查询词典 sequencing

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

Southern blot analysis and partial sequencing showed that the candidate BAC clones actually contained pufferfish homologue of

作者相信，本河豚鱼基因组BAC文库定会在河豚鱼基因组研究和比较基因组学的研究中发挥重要作用。

However, with the advent of new sequencing technologies, genomics research is now at the threshold of a second revolution.

但是，随着新的测序技术的到来，基因组学研究正处在第二次革命的开始。

Objective To develop a new method for amplifying and sequencing the full-length of HBV genome.

此方法有很大的局限性，不能反映单一感染株基因组序列的实际情况。

The results obtained by sequencing of the 10 PCR products and chip arraying were almost the same, the only differe

HBV基因芯片可初步用于HBV DNA 检测，可能是临床追踪评价抗病毒治疗效果的较好方法之一。

Otherwise, the algorithm restores the domain of variables that were eliminated due to the instantiation of current variable, and backtracks sequently. This thesis gives design for "Ming Yue "[1.1], and briefly states impleme -nt of problem solving system on discrete domains combined with examples. It also gives some experiment results of typical problems, such as car sequencing, timetabling problem, job shop scheduling problem and so on.

本文给出了&明月&的设计，并且结合实例简述了离散论域上约束求解系统的实现，给出了一些典型问题的实验结果，如汽车排序、时间表调度问题、车间作业调度问题等，实验证明该系统对于某些类问题的确是行之有效的。

Based on the cDNA library, 862 ESTs were obtained through batched sequencing, and 68 "Contig" and 184 "Singlton" were produced from the assembled ESTs.

基于构建好的中蜂cDNA文库，利用批量化ESTs测序策略，成功得到了质量较好的有效ESTs序列862条，经拼接后分别得到&Contig&和&Singleton&序列68个和184个。

The joint research project with the Chinese Academy of Science,"Trans-genetic Somatic Cloned Goat", was bulletined as the No. 1 Fundamental Research News Report in 1999."Precise Sequencing of Rice No. 4 Chromosome" project came on top of the 10 Scientific Progress News Report in 2002. 3 research achievements have won the second prize of the National Scientific Development Award.

其中，由中科院与我校共同完成的&转基因山羊体细胞克隆山羊&成果被评为1999年中国基础研究十大新闻之首，由我校参与完成的&水稻第四号染色体精确测序&工作被评为2002年中国十大科技进展头条新闻，3项成果荣获国家科技进步二等奖。2002年以来有3篇论文被评为全国优秀博士学位论文。

The genotype of the separation was J, which was clewed by sequencing analysis.

测序结果证实为水痘带状疱疹病毒，基因型为J型。

With the strong data—handling capacity of C—dBase III, bibliographic term sequencing, sequence number determination, supplementation of composing commands and comparting characters can all be processed by computer with a little manual operation. It greatly increases operation efficiency in editing...

该方法利用汉字dBascⅢ输入、编辑文献条目，并借助其强大的数据管理功能，完成文献条目排序和条目顺序号、排版命令、内容分隔符的添加，利用排版软件进行计算机排版，使大量的手工操作转由计算机自动执行，从而大大提高了工作效率。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。