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Three genes specified expressed in hybrids and one gene over-expressed in hybrid were found. By cloning, sequencing and BLAST analysis, one specific expression cDNA band was cytosolic isocitrate dehydrogenase gene, the other two were new genes which function were unknown, and the over-expressed cDNA band was proteosome subunit p112 gene.

发现了 3 个杂种鸡特异表达的基因和 1 个杂种鸡表达增强的基因，进一步克隆、测序和比对分析初步表明：杂种特异表达的 cDNA 片断分别为鸡细胞质异柠檬酸脱氢酶基因和 2 个未知功能的新基因；杂种表达增强的 cDNA 片断为鸡蛋白酶体亚基 p112 基因。

Methods: HPV 16/18 infection was detected by nested-polymerase chain reaction, the p53 mutation was detected by direct sequencing, and the p53 and the HPV 16/18 E6 proteins were studied using immunohistochemistry on 129 pterygial specimens and 20 normal conjunctivas.

本研究利用巢叠式聚合脢锁反应分析自129例罹患翳状赘肉之翳状赘肉组织及20例正常无翳状赘肉的结膜组织检体中，是否有人类乳突病毒16及18型的感染，并利用直接定序的方式检测p53基因是否发生突变，此外亦以免疫组织化学染色法侦测p53蛋白与人类乳突病毒E6蛋白。

Methods: MUC1/Y extracellular domain was used as a target molecule to biopan Ph. D. 12 phage randem peptide library. Two protocols using affinity gel and cell culture plates respectively were carried out. Positive phage clones were identified by ELISA. ssDNA sequencing was done on 16 positive phage clones to get the amino acid sequences of MUC1/Y-binding peptides. Immunohistochemistry was done to show the capacity and specificity of positive phage clones to bind the tumor cell lines.

以MUC1/Y黏蛋白的胞外段蛋白(MUC1/Yex)为靶分子，用凝胶亲和法和酶联板法分别筛选十二肽噬菌体随机肽库，ELISA鉴定阳性克隆，DNA序列测定后确定MUC1/Yex结合肽的氨基酸序列；免疫组化鉴定阳性噬菌体克隆与正常及肿瘤细胞的结合能力及特异性。

It is estimated that there are about 40,000 functional genes.In order to understand those genes related to human diseases and the novel functional genes, the genetic manipulated mice including transgenic mice and knockout mice have been generated to facilitate the purpose.This transgenic core has been collaborated with industrial and research institutes for years, and has built up competitive know-how for transgenic mice, embryo cryopreservation, and pathogen free rederivation.

小鼠长期以来即为研究人类疾病最佳动物模式，2001年美国赛洛因公司已将小鼠129的两个品系之基因序列解码完成提供付费使用，2003年小鼠B6的基因序列也被Mouse Genomic DNA Sequencing Consortium解码完成，预估约有4万个功能基因，随著基因操控技术的成熟，制造出各种带不同基因缺陷的模式动物供生物医学研究已非难事。

After the recombinant plasmid was certified by DNA sequencing,the conservative region of Ki67 gene was inserted into pEGFP vector reversedly.

方法用基因重组方法将人Ki67基因cDNA保守序列反向克隆到真核表达质粒pEGFP-C1中，构建人反义Ki67核酸载体。

To deepen the results, researchers from Rhodococcus erythropolis LSSE8-1 and Gordon's LSSEJ-1 strain in the successful expansion of desulfurization have been related to the gene fragments, and the sequencing is being carried out desulfurization projects microbial bacteria work to build .

为深化成果，研究人员从红平红球菌LSSE8－1和戈登氏菌LSSEJ－1中成功扩增得到了脱硫相关基因的片断，并进行了序列测定，目前正展开微生物脱硫工程菌的构建工作。

By randomly single-pass sequencing from 5'-end of muscle cDNA library and liver cDNA library from Silkie Fowl, 16600 ESTs of muscle tissue which are more than 100bp and uncontamination and 17733 ESTs of liver tissue which are more than 100bp and uncontamination have been obtained.

利用大规模DNA序列测定方法，对丝毛乌骨鸡胸肌组织和肝脏组织的2个cDNA文库在5'端进行了大规模的单向单次的随机测序，得到了肌肉组织的16600条大于100bp高质量无污染的ESTs和肝脏组织的17733条大于100bp高质量无污染的ESTs。

It has also become a good model system to study cross-talk of different plant hormones with the development of genetics and whole tomato genome sequencing from International Solanaceae Genomics Project.Jasmonic acidis a fatty acid-derived signal and exists in the plant kingdom extensively.

番茄是最重要的蔬菜作物之一，随着番茄遗传学研究和国际茄科基因组计划(International Solanaceae Genomics Project，SOL)的发展，番茄已成为研究植物激素相互作用的理想模式植物。

A 3.12 kb region spaning from exon 13 to exon 15 in the bcr region were screened by DNA pooling and denaturing high performance liquid chromatography, and the results were varified by sequencing.

利用DNA库结合变性高效液相色谱对长度为3.12kb的bcr基因断裂丛集区进行序列变异的筛查分析，并通过测序对筛查结果进行了验证。

ITS2(Intemal Transcribed Spacer 2) region in ribosome DNA has a character of conservative that makes a low aberrance ratio, on the other side, ITS-2 region evolves itself. According to the characters, ITS-2 region sequencing is an effective parameter in taxology and pests-control..

核糖体基因的第二内转录间隔区有较高的保守特性，同时进化速度又较快，因此序列进行测序得出的种内差异，是系统发育学的有力参数，也有助于异色瓢虫分类学和农林虫害的防治。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。