查询词典 sequencing

The results of sequencing was contrasted by BLAST software. The sequence of the PCR products shared 100% homology with Pasteurella multocida reference strain.

设计1对引物进行PCR扩增、克隆之后，送往大连宝生物公司测序，测序结果运用BLAST软件比对，与多杀性巴氏杆菌同源性为100%。

Tuberculosis H37Rv strain was amplified by PCR and cloned to vector pEASY T1. After sequencing, it was subcloned to fusion expression vector pET28a and then transfected to E coli BL21 (DE3). It was found that the LprG protein was successfully expressed after induction with LPTG.

采用聚合酶链反应从结核分枝杆菌H37Rv基因组中扩增出LprG基因，克隆到pEASY T1中，序列测定正确后，再亚克隆到融合表达载体PET28a中，转化入大肠杆菌BL21(DE3)，经IPTG诱导表达。

We expect that the transgenictomato plant become a bio-reactor and develop a new way to product wild-typep53 protein.In this study,the coding sequencing of human wild-type p53tumor suppressor was ligated with the promoter and terminator of phaseolin,which is expressed specially in plant seeds.

本实验试图将人野生型p53肿瘤抑制基因引入番茄植株，通过采收番茄果实很方便的获得p53蛋白，使转基因番茄成为p53蛋白的生物发生器，开辟一条生产p53蛋白的新途径。

The isolated strains were subjected to phenotypical study,salt-aggregation test and rDNA ITS sequencing.

对获取的活性菌株进行形态特征研究、盐需求测试、核糖体rDNA ITS区序列分析，并将所测得序列在NCBI数据库中进行相似性搜索，选取其中相似性较高的序列以邻接法构建系统进化树，将该菌株分类鉴定到种。

Genome and EST sequencing projects generate a wealth of sequence information for agronomically and phylogenetically important plant species of which the majority is difficult to subject to functional genomics.

农艺上和系统发生上重要的植物物种的基因组和EST测序计划产生了大量的序列信息，其中大部分难以服从功能基因组学。

Patients with clinical virologic breakthrough during NA therapy and 4 negative control patients without virologic breakthrough were selected for the study of quasispecies dynamics. All the 70 serum samples from these 10 patients were analyzed with the method of'PCR-cloning-sequencing'. Totally over 2,100 clones were sequenced. The target sequences obtained were aligned, compared and analyzed to study the mechanism of NA drug resistance and to understand the clinical course of NA therapy from a viral populational genetic point of view.

我们进一步采用国际认可的&金标准&&PCR-克隆-测序-序列分析&的方法，对6例发生临床病毒学突破的患者以及4例未发生临床病毒学突破的患者的全程血清样本进行了准种检测，共测序2100余个，以系统深入地对LAM和ETV治疗过程中HBV种群的演变及其临床意义进行探讨。

Genetic studies have been performed on the hereditary diseases of disseminated superficial actinic porokeratosis, familial progressive hyperpigmentation and triphalangeal thumb-polysyndactyly syndrome by linkage analysis and sequencing.

本研究通过连锁分析和直接测序的方法，分别对播散性浅表性光化性汗孔角化症、家族性进行性色素过度沉着症和拇指三指节多指并指多掌骨畸形这三种疾病进行了遗传学分析。

Genetic studies have been performed on the hereditary diseases of disseminated superficial actinic porokeratosis, familial progressive hyperpigmentation and triphalangeal thumb-polysyndactyly syndrome by linkage analysis and sequencing.

中文摘要本研究通过连锁分析和直接测序的方法，分别对播散性浅表性光化性汗孔角化症、家族性进行性色素过度沉着症和拇指三指节多指并指多掌骨畸形这三种疾病进行了遗传学分析。

In attempt to elucidate the molecular mechanism of cold tolerance of wintersweet, which blooms in the deep winter,a COR413 protein gene, designated as Cpcor413pm1, was obtained by sequencing the randomly selected clones, on the basis of Chimonanthus praecox flower cDNA library construction and its ESTs analysis.

为研究蜡梅冬季开花过程的抗寒分子机理，在构建蜡梅花cDNA文库及EST分析的基础上，通过随机克隆测序，克隆了1个蜡梅COR413蛋白的cDNA基因，命名为Cpcor413pm1。

Methods A strain of Propionibacterium acnes from rumen fluid of health cow with permanent rumen fistula under sterile condition was isolated by segregation procedure of anaerobic bacterium and specific medium SLB, and identified by extraction of the genome DNA of isolation bacterium, clone of the 16S rRNA gene, and sequencing of the strain.

无菌采取装有瘤胃瘘奶牛的瘤胃液，按照厌氧菌分离步骤，通过丙酸生成菌株的特异性培养基SLB进行筛选，提取分离菌的基因组DNA，克隆其16S rRNA基因，进行序列测定，分离出一株痤疮丙酸杆菌。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。