查询词典 sequencing

Abortus, amplify BP26 gene by PCR and clone to pMD18-T simple vector. Identify the constructed recombinant plasmid pMDBP26 by sequencing, then subclone to vector pET-28a. Transform the constructed recombinant plasmid pETBP26 to E. coli BL21(DE3) for expression under induction of IPTG. Purify the expressed product by histidine-binding resin column chromatography and identify by Western blot.

提取布鲁氏菌基因组DNA，用PCR扩增出BP26基因，并克隆到pMD18-T载体上，测序正确后，再亚克隆至pET-28a载体上，转化到大肠杆菌BL21(DE3)中，用IPTG诱导表达，经组氨酸结合树脂柱纯化，并对纯化后的表达产物进行Western blot鉴定。

Then, two primers were designed from it, the full length cDNA of malate dehydrogenase gene in Chinese cabbage was gained through RT-PCR amplifying and sequencing and entered GenBank as accession number FJ208590, which is 1 209 bp length and coded 403 amino acids.

然后设计引物，经RT-PCR扩增、测序，获得了大白菜苹果酸脱氢酶基因的cDNA全长序列，并已将其登录到GenBank，登录号为：FJ208590，该cDNA全长1 209 bp，编码403个氨基酸。

The central struggle was between the publicly funded international Human Genome Project, led by Francis Collins, a distinguished scientist and devout Christian, which pursued a slow-and-steady approach to sequencing until Celera, a private company led by Craig Venter, appeared on the scene and boldly claimed that its revolutionary new technique, whole-genome shot-gun sequencing, could do the job in less than half the time.

主要竞争存在于公众基金的国际人类基因组项目及Celera公司之间。前者由弗朗西斯。克林斯领导，克林斯本人是位杰出的科学家和虔诚的基督徒。它一开始是寻求一种慢而稳定的方法来测序，但是这种情况在Celera公司出现之后就改变了。Celera公司在Craig Venter的领导下，大胆地主张它的革命性的新技术&whole-genome shot-gun&测序法，该法可以节省一多半的时间。

Dna sequencing of the representative pcr products of carboxyl-terminus of lmp1 gene was analyzed by using four-colored fluorescence terminator sequencing technique.

具有代表性的lmp1基因羧基末端pcr产物采用四色荧光终止序列技术进行dna序列分析。

By mitochondrial DNA control region sequencing the relationship of haplotype phylogenies can be reconstructed to distinguish the Taiwan ring-necked pheasant and imported ring-necked pheasant. Additionally, the sequencing can be used to analyze genetic diversity and genetic structure. The results are expected to provide the reference base for identifying and conserving Taiwan ring-necked pheasant.

本论文研究结论建议，未来进行原生环颈雉族群保育或人工复育计画时，可利用外部形质测量分析、粒线体DNA基因型与遗传多样性分析，做为环颈雉各原生或外来族群的个体辨识工具，并提供拟订族群保育及复育经营措施之参考依据。

Chinese scientists have contributed to the genome sequencing of a rice paddy, silkworm, hen and pig. In October last year, they finished sequencing the first Han Chinese genome, Yang said.

成都大熊猫繁育研究基地研究中心主任侯蓉说：&我们相信，大熊猫基因组的研究将为濒危物种的保护和人工繁育提供新的途径。&

L By looking at the individual elements in any given backbend—such as examining the direction of movement of the shoulders, arms, torso, hips and legs—we can deconstruct the posture and aligning, strengthening and opening these individual regions to more refined by identifying particular areas of interest need in our students' bodies and focusing on these areas in class, I call this "progressive sequencing" and it is different from "pose/ counter pose " sequencing.

通过关注每个后弯体式中独立的元素——例如检查肩膀、手臂、躯干、髋以及两腿的运动趋势——我们能够通过分解的方式来探究体式和学习校准；通过了解学生的身体中需要深化和发掘的独立的区域，强化并打开这些独立的区域，并且能够在课堂中专注于这些区域，我把这个叫做&渐进序列&，这与&体式/反体式&序列是不同的。

By looking at the individual elements in any given backbend—such as examining the direction of movement of the shoulders,击 arms,击 torso,刨 hips and legs—we can deconstruct the posture and aligning,凋 strengthening and opening these individual regions to more refined by identifying particular areas of interest need in our students' bodies and focusing on these areas in class,刞 I call this "progressive sequencing" and it is different from "pose/ counter pose " sequencing.

通过关注每个后弯体式中独立的元素——例如检查肩膀、手臂、躯干、髋以及两腿的运动趋势——我们能够通过分解的方式来探究体式和学习校准；通过了解学生的身体中需要深化和发掘的独立的区域，凌强化并打开这些独立的区域，凚并且能够在课堂中专注于这些区域，凟我把这个叫做&渐进序列&，凛这与&体式/反体式&序列是不同的。刦

By looking at the individual elements in any given backbend—such as examining the direction of movement of the shoulders,删 arms,凹 torso,凨 hips and legs—we can deconstruct the posture and aligning,凟 strengthening and opening these individual regions to more refined by identifying particular areas of interest need in our students' bodies and focusing on these areas in class,凝 I call this "progressive sequencing" and it is different from "pose/ counter pose " sequencing.

通过关注每个后弯体式中独立的元素——例如检查肩膀、手臂、躯干、髋以及两腿的运动趋势——我们能够通过分解的方式来探究体式和学习校准；通过了解学生的身体中需要深化和发掘的独立的区域，凨强化并打开这些独立的区域，凣并且能够在课堂中专注于这些区域，凡我把这个叫做&渐进序列&，凷这与&体式/反体式&序列是不同的。凯

By looking at the individual elements in any given backbend—such as examining the direction of movement of the shoulders,凸 arms,凊 torso,凳 hips and legs—we can deconstruct the posture and aligning,刨 strengthening and opening these individual regions to more refined by identifying particular areas of interest need in our students' bodies and focusing on these areas in class,凙 I call this "progressive sequencing" and it is different from "pose/ counter pose " sequencing.

通过关注每个后弯体式中独立的元素——例如检查肩膀、手臂、躯干、髋以及两腿的运动趋势——我们能够通过分解的方式来探究体式和学习校准；通过了解学生的身体中需要深化和发掘的独立的区域，凢强化并打开这些独立的区域，凉并且能够在课堂中专注于这些区域，刁我把这个叫做&渐进序列&，刘这与&体式/反体式&序列是不同的。刓

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。