查询词典 sequencing

It was Eric Lander,while working for Colins in the tortoise-paced Human Genome Project, who saw that his team was losing and made it his business to beat Venter's harelike private venture at its own game .With $34 milltortoise-paced Human Genome Project, who saw that his team was losing and made it his business to beat Venter's harelike private venture at its own game .With $34 million from the Gemome Project and a $38 million loan from the Massachusetts Institute of Technologh's Whitehead Institute,Lander ordered dozens of special-purpose computers and state-of-the art capillary machines and built automated gene-sequencing pipeline so insatiable that he was soon grabbibg long stretches of DNA from other labs to fees its monstrous appetite .It was his lab's work that brought the race to a photo finish, and it was his name that appeared first on the Nature article that published the results.

这是埃里克Lander，当工作为Colins 在草龟踱步的人的染色体项目，看见他的队是丢失的并且做它他的事务摔打Venter's harelike 私有事业在它自己的游戏With $34 milltortoise 踱步了人的染色体项目，看见他的队是丢失和做它他的事务摔打Venter's harelike 私有事业在它自己的游戏With $34 百万从Gemome 项目和一笔$38 百万贷款从Technologh's Whitehead 学院马萨诸塞学院， Lander 定购了许多专用计算机和状态这艺术血丝机器和修造了自动化基因程序化管道很贪心他是grabbibg 长期很快舒展DNA 从其它实验室对费它是带来种族给相片结束的他的lab's 工作，并且这是出现首先在自然文章出版结果的他的名字。

HaNPV is the earliest commercial baculovirus insecticide in China and about 100, 000 hectare cotton farmland had been used such insecticide to prevent Heliothis armigera by the end of 1999. Many aspects of HaNPV such as the isolation and identification of different virus strains, bioassay of their toxicity, infectivity and duplication in culture cells, genome sequencing and some functional genes have been researched.

国内已对HaNPV进行了多方面的研究，包括病毒株的分离鉴定、形态特征、病毒生物活性测定、离体细胞感染和复制、测序和功能基因研究，基因工程病毒杀虫剂的构建等；但对其复制侵染与宿主细胞互作的分子机理，目前仍缺乏足够且深入的了解。

After the NK gene from recombinant plasmid pUC19-NK was cloned on expression vector pBV220, it was transformed into host cell E. coli HB101. Recombinant plasmid pBV220-NK was extracted for identifying by analysis of restriction enzymes, PCR and sequencing. Studies on recombinant strain's growth and expression showed that heterogene have no distinct effect on host cell. The recombinant plasmid have excellent segregational stability but low structural stability. The results of kinetics of pBV220-NK expression in E.

将重组质粒pUC19-NK上的纳豆激酶基因成功克隆到表达载体pBV220上，并将之转入大肠杆菌HB101中，得到转纳豆激酶基因工程菌，提取重组质粒经单酶切、双酶切及PCR分析验证后，对重组菌的生长及表达进行一系列研究，结果表明：外源纳豆激酶基因的导入对宿主的生长没有明显的影响：重组质粒的稳定性实验表明：该质粒具有良好的分离稳定性，而结构稳定性较差。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

The psbL-psbJ region of chloroplast DNA of 8 and 2 Hordeum species or subspecies from China and other countries, respectively, were investigated by PCR amplification and direct sequencing.

对8份来源于中国和2份来自国外的不同大麦属物种或亚种叶绿体基因组psbL～psbJ区进行了序列测定。

The recombinant plasmids were analyzed by sequencing and the one containing the correct fragment was transfected into K562 cells. The condition of idiotypic protein expression was tested by Western-Blot.

将经测序鉴定正确的重组质粒转染K562细胞，Western-Blot等方法检测其蛋白表达的情况。

Classic rough set approach acquires knowledge by means of indiscernibility relation, but it is powerless to resolve the sequencing classification problems which contain qualitative and quantitative attributes as well as criterias.

经典粗糙集方法是通过不可区分关系来获取知识的，但它对定性属性、定量属性以及准则属性同时出现的定序分类问题就显得无能为力。

Based on the present findings, it appears that all the Taiwan WAIS-Ⅲ working memory index, the single Arithmetic and the Digit Span subtests, and the span scores of the Digit Span subtest could adequately discriminate individuals without working memory impairment, but had poor sensitivity on detecting the patients who evidenced working memory impairment. Accordingly, it is suggested that clinicians should gauge the working memory index by a composite of the Arithmetic, the Digit Span, and the Letter-Number Sequencing subtests if possible. Otherwise, a caveat should be taken with care to interpret the working memory index, as estimated by a composite of the Arithmetic and the Digit Span subtests, because the chance of the increasing false negative rate becomes ineludible.

综合上述，显见WAIS-Ⅲ中文版中之工作记忆指数、单一算术及单一记忆广度分测验、或记忆广度分测验之各项广度值在区分患者有无工作记忆功能异常上皆拥有良好之区辨力，但对侦测工作记忆功能异常患者的敏感度普遍不佳，建议临床工作者应尽量取得算术、记忆广度、与数－字序列等三项分测验组合所估算之工作记忆指数；若在有限条件下，临床工作者仅能以算术与记忆广度两项分测验之组合所估算之工作记忆指数来评估受试者工作记忆，应留意假阴性比例上升的问题。

Pro-opiomelanocortin plays an important role on animal ingestive behavior and energy homeostasis. Genetic variation of POMC was detected by PCR-SSCP and DNA sequencing techniques in 480 individuals from seven cattle breeds (i.e., Qinchuan, Nanyang, Jiaxian Red cattle, Jinnan, Luxi, Angus, and Chinese Holstein), and association analyses were carried out to evaluate the effects of genotypes of the candidate genes on growth traits in Nanyang cattle.

为研究阿片黑皮质素前体在动物采食和能量平衡调控中发挥重要作用，文章采用PCR-SSCP结合DNA测序方法，对秦川牛、南阳牛、郏县红牛、晋南牛、鲁西牛、安格斯牛和荷斯坦奶牛共计480头个体POMC基因的多态性进行研究，并分析了多态位点与南阳牛生长性状的相关性。

In combination with sequencing data, these results show that insertional MinD mutant could cause polar effect on MinE expression.

从以上的结果与序列比对结果推测，本实验构筑的minD突变株可能发生polar effect而影响MinE的表现。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。