查询词典 sequencing

Methods Forty-two Gram-positive coccal isolates were acquired from infection cases in Luoyang, Jiyuan, Xuchang and Nanyang of Henan Province, and were analysed by means of 16S rRNA gene sequencing and Vitec-32 automatic identification system respectively.

对从河南洛阳、济源、许昌、南阳等地送检病猪体内分离到42株革兰阳性球菌分离纯化后，分别用16S rRNA基因序列和Vitek-32全自动细菌鉴定系统对其进行鉴定。

Forty-two Gram-positive coccal isolates were acquired from infection cases in Luoyang, Jiyuan, Xuchang and Nanyang of Henan Province, and were analysed by means of 16S rRNA gene sequencing and Vitek-32 automatic identification system respectively.

将从河南洛阳、济源、许昌、南阳等地送检病猪体内分离的42株革兰阳性球菌纯培养后，分别用16S rRNA基因序列比较和Vitek-32全自动细菌鉴定系统进行鉴定。

The active imaging system based on range gated compart different range background light and reflected light by target according to time sequencing by using a pulsed laser and intensifilered CCD camera, to corfirm the pulse signal which the target reflect fall on working time of gated camera.

基于距离选通的激光主动成像系统是利用脉冲激光器和选通增强型CCD相机，以时间的先后分开不同距离上的背景散射光和目标反射光，使由被观察目标反射回来的辐射脉冲刚好在选通相机选通工作的时间内到达相机并成像。

Phycobiliprotein are important light-harvesting pigment proteins in Cyanophyceae, Rhodophyceae, Cryptophyceae and a little Pyrrophyceae.

中文题名极大螺旋藻藻蓝蛋白基因的克隆及其表达研究副题名外文题名 Cloning and sequencing of the phycocyanin gene from Spirulina maxima and its over-expression in Pichia pastoris 论文作者于平导师岑沛霖学科专业生物化工研究领域\研究方向学位级别博士学位授予单位浙江大学学位授予日期2003 论文页码总数110页关键词藻胆蛋白基因克隆藻蓝蛋白基因螺旋藻馆藏号BSLW /2003 /Q949 /32 藻胆蛋白是蓝藻、红藻、隐藻和一些少数甲藻中的一种重要的捕光色素蛋白。

Sequencing of this 4.3kb DNA fragment revealed that there is a β-glucosidase gene urnbgl3A with an ORF of 1956bp, encoding product shared 63% identity and 79% similarity with a beta-glucosidase (GenBank NO:AAX16378.1) of uncultured murine large bowel bacterium BAC 31B, and shared 50% identity and 67% similarity with a beta-glucosidase-related glycosidases (GenBank NO:ZP-00308419.1) of Cytophaga hutchinsonii.

测序结果表明在4.3kb的片段上有一个全长为1956bp的ORF，编码一个可能的β-葡萄糖苷酶基因umbgl3A，其编码产物与一个来源於小鼠大肠未培养细菌的β-葡萄糖苷酶(GenBank Acession NO：AAX16378.1)一致性为63%、相似性为79%；与哈氏噬纤维菌的β-葡萄糖苷酶(GenBank Acession NO：ZP-00308419)的一致性为50%、相似性为67%。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

By sequencing, we found that ITS1 and ITS2 length of Desmodium sequax is 225 bp and 197 bp.

经过定序之后，得到波叶山蚂蝗的ITS1序列长度为225 bp， ITS2序列长度为197 bp。

Sequencing and mutation detection in collaboration with Dr. Dunlap have localized prd-4 mutation.

和美国 Jay Dunlap合作进行了测序和突变分析，获得了生物钟prd-4基因。

Methods Eight coding exons of ED1 gene of two patients with clinically confirmed X-linked hypohidrotic ectodermal dysplasia, their parents, and 100 unrelated population-matched control were amplified by polymerase chain reaction. The products were further analyzed by direct sequencing.

采用聚合酶链反应扩增该家系中两名临床诊断为X性连锁少汗性外胚叶发育不良的患者、患者父母以及100例健康对照者ED1基因的8个外显子，进行DNA直接测序。

Seted up professional nurse team for endoscopy, scientific, normalized and sequencing regulation was adopted, emundation and sterilisation carried by nurse, infection control was carried by special messenger, all the index was statistical analysised.

成立内镜手术专科护士小组，采取合理调配使用的科学化、规范化、程序化管理模式，实行专人清洗消毒，专人进行感染控制监测的操作规程，并对设置前后各项指标进行比较。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。