查询词典 sequencing

Methods Bisulphate sequencing was applied to analyse the mcthylation of the CpG islands in the SNRPN gene locus.

方法应用重硫酸盐测序法对一例临床高度疑似PWS的患者及其父母的15q11-q13印迹中心SNRPN基因alpha外显子的10个CpG位点的甲基化情况进行分析。

To find methylated genes, researchers usually turn to a technique called bisulphite sequencing, which chemically changes normal cytosine to thymidine (T; another DNA base), while leaving methylated cytosines unchanged.

研究者通常借助于亚硫酸氢盐测序技术检测甲基化的基因，它通过化学反应使正常胞嘧啶转变为胸腺嘧啶，而对甲基化的胞嘧啶无作用。

In the NPC cell lines,loss of BLU expression correlated with hypermethylation of the 20 CpG sites in the 5′ region of BLU(approximately -292bp to -23bp upstream the translation start site)as revealed by bisulphite sequencing. Expression of the gene was restored aftertreatment with 5-aza-2-deoxycytidine in CNE2, suggesting that repression of BLUtranscription might at least be partially mediated by promoter methylation.

结果显示，BLU基因翻译起点ATG上游-292--23bp区间内的20个CpG位点在5例细胞系中均被完全或者部分甲基化，与基因的表达缺失呈负相关关系；去甲基化试剂5-Aza-CdR处理CNE2后基因表达部分恢复，说明甲基化至少是引起BLU基因转录抑制的部分原因。

The total RNA was abstracted from the larvae of Boophilus microplus, and a 1982bp Bm86 gene was amplified by RT-PCR. The target gene was subcloned into T vector. The sequencing showed that the nucleotide sequence of the cloned Bm86 gene shared 97.4% homology with the data published in and this fragment contained the complete open reading frame of Bm86 gene.

为了克隆微小牛蜱Bm86 基因及构建该基因的表达载体，以微小牛蜱饥饿幼蜱的破解物提取的总RNA为模板，参照已发表的微小牛蜱Bm86基因的核苷酸序列，设计了1对引物，采用RT-PCR技术获得微小牛蜱Bm86基因；将Bm86基因克隆入载体，并进行序列分析，结果证明，克隆的Bm86基因序列与GenBank上登录的Bm86基因序列的同源性达97.4%，而且该序列包含完整的开放阅读框。

Methods We used the automatic DNA sequencing machine (Model 377) to detect the nucleotide sequence of the inserted part of the recombinant plasmid pBX1 from Borrelia burgdorferi B31 strain.

方法采用377型DNA自动测序仪对莱姆病螺旋体重组质粒pBX1的插入片段进行DNA序列测定，并通过计算机软件对其进行限制性内切酶酶谱分析。

Results ①DNA sequencing showed that pBX1 contained a 477bp inserted gene fragment,and when it was compared with the published sequence of the specific region of the gene of the 83kd antigen protein from Borrelia burgdorferi B31 strain,only one amino acid codon was different.

结论该研究成功地对莱姆病螺旋体83kd抗原蛋白特异性区段进行了基因重组和表达，为进一步研究其在莱姆病血清学诊断和基因工程亚单位疫苗研制中的应用奠定了基础。

We obtained complete coding sequence of myostatin gene from Bos taurus, Bos indicus, Bos grunniens, Bos frontalis and Bubalus bubalis (swamp buffalo and river buffalo) by PCR fragments sequencing method. The molecular evolution characters of CDS of myostatin gene were analyzed.

运用PCR分段扩增测序法，获得普通牛、瘤牛、牦牛、大额牛、水牛MSTN基因编码区全序列，进而分析编码区序列的分子进化特征。

Parvifolia was 642bp by cloning PCR production and sequencing, and its phylogenetic information might facilitate to provide the direct molecular evidence to clarify its origion and evolution for Buxus sinica var. parvifolia.

珍珠黄杨ITS片段克隆测序后获得的序列长度为642bp，其系统学信息将为珍珠黄杨的起源进化提供有力的分子水平证据。

Results: Based on the study mentioned above, about 323 bp positive band were amplified under the anneal temperature of 65℃ by total volume of 25 25ul PCR reaction. Sequencing results prove the positive band were fragments of Cytb genes from both Cervus elaphus Linnaeus and Cervus nippon Temminck.

结果：在方法学考察的基础上，在25ul PCR反应体系，退火温度为65℃的条件下，能准确扩增出约323bp 大小的阳性DNA条带，经测序验证结果表明，系正品鹿茸原动物的Cytb基因序列片段，而伪品鹿茸未扩增出条带。

Partial sequences of chalcone synthase gene exon 2 were amplified by PCR method from genomic DNA of 10 species of the basal eudicots. After cloning and sequencing, 26 seqences were obtained.

用PCR方法从目前极少被报道的真双子叶基部类群10种植物的总DNA中扩增出CHS基因外显子2的部分序列进行分析，经克隆后测序，共得到26个不同的片段。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。