查询词典 sequencing

Results The activity of FⅫ were 52.5%, 78.6% and 89% for the proband, his father and mother, respectively. Direct sequencing suggested that the proband was 46 T/T genotype in exon1, while both his parents were 46 C/T genotype in the same alle.

结果 先证者及其父、母FⅫ：C分别是52.5%、78.6%、89%，直接测序结果提示先证者F12基因的第1外显子46位碱基即启动子上游-4位为T/T基因型，其父、母在该位点均为C/T基因型。

If Sequencing Batch Reactor was used directly to treat anaerobically digested effluent, the treatment efficiency was very poor, with only 8.31% for COD removal rate, and 78.7% for NH 3 N.

采用序批式活性污泥法工艺直接处理厌氧消化液，污染物的去除效果很差，COD仅去除8.31%，NH3-N去除7 8.7%。

Design, Setting, and Patients After exposure to an index patient whose fatal illness was characterized by fever and hemorrhage at a primary care hospital and regional tertiary care hospital's isolation ward, secondary cases with febrile illness who were suspected of being exposed were tested for antibodies against Anaplasma phagocytophilum and by polymerase chain reaction and DNA sequencing for A phagocytophilum DNA.

HGA1990年在美国被创造，在欧洲被创造的时间则是在1997年。据报道，每年该疾病的感染人数在持续增加。有关数据显示，该种疾病在风行地区的感染率高达15%至36%，这意味着对该种疾病的诊断由于感染程度稍微或者是患者没有症状经常被遗漏。

Design, Setting, and Patients After exposure to an index patient whose fatal illness was characterized by fever and hemorrhage at a primary care hospital and regional tertiary care hospital's isolation ward, secondary cases with febrile illness who were suspected of being exposed were tested for antibodies against Anaplasma phagocytophilum and by polymerase chain reaction and DNA sequencing for A phagocytophilum DNA.

HGA1990 年在美国被发现，在欧洲被发现的时间则是在1997年。据报道，每年该疾病的感染人数在持续增加。有关数据显示，该种疾病在流行地区的感染率高达15%至 36%，这意味着对该种疾病的诊断因为感染程度轻微或者是患者没有症状常常被遗漏。

Design, Setting, and Patients After exposure to an index patient whose fatal illness was characterized by fever and hemorrhage at a primary care hospital and regional tertiary care hospital's isolation ward, secondary cases with febrile illness who were suspected of being exposed were tested for antibodies against Anaplasma phagocytophilum and by polymerase chain reaction and DNA sequencing for A phagocytophilum DNA.

2006年11月9日与17日之间在中国安徽省的一家地区医院中，为数9人的具有发烧和其它症状的病人被诊断患有HGA。这些病人都没有被蜱叮咬过。但所有这9位病人都在指标病例死亡前12小时内与其有过接触，该指标病例被怀疑患有致命性HGA，她出现了大出血并接受了气管内插管（即将一根柔韧的塑胶管子置放在气管中以帮助肺部的通气。）。

Methods With polymerase chain reaction-single-strand conformation polymorphism combining argentation and glue retrieval, DNA sequencing, and restriction fragment length polymorphism, the SNP of the 50 exons in all the coding regions of ABCA1 gene was detected in 112 patients with established diagnosis of coronary heart disease.

通过聚合酶链反应－单链构象多态性分析结合银染后胶回收、DNA测序和限制性内切酶酶切分析方法检测112例明确冠心病诊断患者的ABCA1基因全部编码区50个外显子的SNP。

Methods PCR-SSCP and PCR product cloning, sequencing were performed to detect mutation of p16 gene exon 2 in peripheral blood of 60 patients with arseniasis caused by coal-burning pollution,at the same time,PCR-based methylation assay was performed to analyze the methylation of p16 gene exon 1. Results No p16 gene exon 2 mutation was found in 60 cases.

采用聚合酶链反应-单链构象多态性分析和PCR产物克隆测序技术对60例砷中毒患者外周血中p16基因第2外显子突变情况进行检测，同时采用甲基化敏感的限制性内切酶方法对p16基因第1外显子甲基化情况进行分析。

The paper researched by 24 Saccharomyces cerevisiae strains and three kinds of related ascomycetous yeasts in the Angel Yeast Co.,L td,by electrophoresising and sequencing, ribosomal DNA ITS-1 sequences can analysis their's phylogenetic systematics relationship.

本论文以安琪酵母股份有限公司有代表性的酿酒酵母属中24株酿酒酵母及相关3种子囊菌酵母为研究对象，通过对所选取酵母菌株的核糖体DNA ITS-1序列进行电泳和克隆测序，分析出它们之间的系统学关系。

Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

将PCR产物进行重组，并将阳性重组质粒，应用M13测序系统对产物进行DNA序列分析，并与MPM-129株P1基因核苷酸进行同源性比较，发现有4个位置的碱基发生了变化，其同源性为98.8％，证实了PCR所扩增DNA片段的准确性和特异性，同时也证实了不同MP组型的P1基因即使在保守区也存在着一定的差异，将克隆的目的DNA片段用异羟基洋地黄毒苷配基用随机引物法标记制备MP DNA探针，杂交后用碱性磷酸酶标记的抗Dig多克隆抗体与杂交体反应，再用BCIP和NBT呈色，制备MP DNA探针，鉴定所扩增片段的特异性，与同位素探针比较，Dig探针不受半衰期限制，可反复使用，而且价格低廉，值得推广使用。

Methods the 32 variants of bartonella isolated in yunnan province and published in genbank from 1999 to today were taken as the target strains. the most closely related typical strains searched by blast in genbank with sequencing program were taken as comparative stains and their glta gene sequences were compared each other.

用云南省1999年至今分离到的并已在genbank中公布的32个巴尔通体变异体为目标株，利用网上核酸序列比对程序blast进行核酸序列同源性搜索，调出相似性最高且有效发表种典型菌株为对比株，进行比对和系统发育分析。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。