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In this study, the DNA fragments coding for amino acids 133～158 of VP1and 20～34 of VP4protein of type Asial FMDV were chemically synthesized and ligated into a tandem repeat 133～158-20～34-133～158. The sequences of signal peptide of Igκ chain and Kozak were fused to the 5'end of this tandem sequence and synthetic oligodeoxynucleotide containing CpG-ISS was fused to downstream of terminal coden of this tandem sequence. And then this long fragment was cloned into the eukaryotic expression plasmid pHook-2, forming a new secreted expression plasmid, named pAS1-E.

在第二章证明了亚洲Ⅰ型口蹄疫病毒VP1蛋白中133～158位氨基酸残基确是一重要B细胞中和抗原表位的基础上，依据第一章获得的口蹄疫病毒亚洲Ⅰ型VP1 cDNA序列及已报道Asial VP4序列，采用真核偏爱密码子化学合成了VP1中编码133～158位氨基酸及VP4中编码20～34位氨基酸这两个抗原表位基因，将其组成133～158-20～34-133～158串联结构，在其5′端加上鼠Igκ链信号肽序列，在翻译调节区加上增强表达的Kozak序列，同时在133～158-20～34-133～158串联结构3'端终止密码子下游加上CpG免疫刺激序列，将这些片段连接后，克隆到真核表达载体pHook-2上，构建成功了DNA疫苗重组表达载体pAS1-E。

The significant sequence change was found in LTR (including U3 13.2%, R7.5% and U5 5.1%), env (2.7%) and ORE S2 (3.9%) between DLA-EIAV and EAV L,while the predicted amino acid sequence difference of Env and S2 protein from the twostrains were 4.4% and 8.8%, respectively.

EIAV L株与国外已发表的一些相关毒株相比核苷酸序列差异很大，同源性只有79%，RTase的氨基酸同源率只有86%，Gag蛋白的的同源率只有79%，说明EIAV L株与国外已报道的几个EIAV毒株的亲缘关系较远。

The PrP of bastard halibut consists of 472 amino acid residues, weighing about 49.6kD. The deduced amino acid sequence is 71.9% similar to that of Japanese pufferfish and 66.4% similar to that of Atlantic salmon, and bears the structural signatures of PrPs including a signal sequence, tandem repeats, a hydrophobic region, two glycosylation sites, a disulphide bridge and a glycosyl phosphatidylinositol anchor site.

推测的牙鲆蛋白质感染因子蛋白由472个氨基酸组成，分子量约49.6kD，具有信号肽、短肽顺接重复、疏水区、糖基化位点、二硫键、糖基缩醛磷肌醇锚定位点等蛋白质感染因子蛋白特征结构域，与红鳍东方鲀和大西洋鲑蛋白质感染因子蛋白的相似性分别为71.9%和66.4%。

This group of proteins cleaves DNA without sequence specificity but with sequence preferences.

尽管具有序专一性，非专一性内酶对於某些特定的碱基，却具有序偏好性。

The proposed algorithm first detects smooth intervals based on gray variance from the original frame sequence, then concatenates them to constructs a new frame sequence. After that, video features such as pixel-wise difference, HSV histogram difference and edge histogram difference are fed to a SVM classifier to decide the types of different shot boundaries.

第一级分类器根据视频帧灰度方差特征，将无明显变化的视频序列从原始视频序列中分离出去，得到一个新的视频序列；第二级分类器在新视频序列的基础上，提取视频图像的像素对差值、HSV空间颜色直方图的各分量差值以及边缘直方图X，Y分量差值等视频特征，并采用支持向量机多分类策略进行镜头边界类型的检测。

When a new gene is identified and its DNA sequence deciphered, it is still unclear what its corresponding protein sequence is.

当一个新基因被识别，其DNA序列被解读，人们仍旧无法搞清相应的蛋白序列是什么。

The specific band EM433 was recovered., cloned and sequenced, and 2 primers of Sequence characterized amplified regions markers, 5 --CGAGACGTCCGAGTC GATATAGC-3 - and 5 --GTCGTCCTTCTAGTGGGTATCCTCC-3 -, were deviced and systhesised in terms of the result of sequence. 41 strains of V dahliae were amplified specifically, 9 nondefoliating strains and 2 defoliating strains could yield EM.

将特异片段EM_(433)进行回收、克隆、测序，在此基础上设计并合成两条SCAR标记引物5&-CGAGACGTCCGAGTCGATATAGC-3&和5&-GTCGTCCTTCTAGTGGGTATCCTCC-3&，对41个V.dahliae进行扩增，10个非落叶型菌系中有9个菌系可以扩增出特异片段，31个落叶型菌系中29个菌系无此特异片段，鉴定准确率可达90％以上。

According to the heavy metal content ,the quality of the flocculated samples and the water samples after flocculation was evaluated by National Standard. The results showed that the heavy metal pollution was most serious in Spring s sullages , then was in Summer 、 Autumn、 Winter in sequence;the water quality in Lianyungang、 Xiexinggang was best in Summer, then Winter、 Autumn and Spring in sequence, there was little difference of water quality in Ancient Canal、 Hengcaolugang and Hongze Lake .

对絮凝前后泥样和水样中的重金属分别根据国标进行了评价，不同的季节，污染最严重的是春季淤泥，然后依次为夏季、秋季、冬季；连云港、协兴港地区的水质在夏季最好，其次为冬季、秋季、春季；古运河、横草路港、梅梁湾、洪泽湖水质的季节性变化并不大。

It is not safe to modify the sequence being iterated over in the loop this can only happen for mutable sequence types

在迭代过程中修改迭代序列不安全（只有在使用链表这样的可变序列时才会有这样的情况）。

Based on modeling each frameof the sequence using TIP technique, we reconstruct the pseudo model of the scene aboutthe image sequence by mosaicking the models to a global model or using a switchingstrategy; then walkthrough can be done in the pseudo model freely.

在对序列图像的每一幅都建模的基础上，我们通过模型拼接或者模型切换生成，建立有关图像序列场景的伪模型，然后实现了在伪模型内任意漫游。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷