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Signal peptide sequence was removed lest it couldnt be recognized by Bacillus subtilis, gene coding for mature peptide was ligated to downstream of sacB signal peptide sequence of pWB980 to form a new ORF and generate pWB980-LipA, gene coding for its chaperone was also amplified and ligated to the downstream of LipA to generate a secretion/expression vector pWB980-LipAB.thechaperone gene was sequenced and analyzed by multiple alignments, resulted showed that there were mutations of nucleotides(1～7) and amino acids (1～2) with the other reported genes.

为了防止枯草芽孢杆菌信号肽酶不能识别脂肪酶的信号肽，切除掉脂肪酶的信号肽编码序列，与枯草芽孢杆菌分泌表达质粒pWB980连接并与质粒上的SacB信号肽构成一个完整的开放式阅读框，获得重组质粒pWB980-LipA，因为该脂肪酶的活性表达需要一个特异性分子伴侣帮助折叠成有活性的构象，将脂肪酶分子伴侣基因串连到脂肪酶基因的下游，获得重组分泌表达载体pWB980-LipAB并且测序分析分子伴侣的基因序列和氨基酸序列并与其它报导的序列进行了比对，发现有1到7个碱基的差异和1到2个氨基酸残基的突变。

The Dahalajunshan Formation dominated lithologically by calc-alkaline volcanic rocks constitutes a complete island arc volcanic sequence; whereas the Aikendaban Formation with dominance of alkaline-subalkaline volcanic rocks belongs to shoshonitic sequence formed in the extension of collision-orogeny.

大哈拉军山组火山岩以钙碱性系列为主，构成了一个完整的岛弧演化序列；艾肯达坂组火山岩以偏碱性-碱性系列为主，属于典型的碰撞造山伸展期的橄榄安粗岩系。

Therefore the nonrandom distribution of 3′ end sequences of effectual primers in tomato RAPD reflected the sequence-specific structure may occur in tomato genome at flank of polymorphic regions. It is probably an attribute of tomato genomic sequence and is common in Lycopersicon.

RAPD引物3′端序列的非随机分布，反映了番茄基因组内的多态性区段侧翼具有序列的特异性，这种特异性结构应是番茄基因组序列的一种属性，这种属性为番茄属基因组所共有。

In general, overall nucleotide sequence identity between two distinct viruses are less than 89%, and strains of virus must have sequence identity greater than 89%, so VA2 is a distinct begomoviruses. In accordance with the revised proposal for naming geminiviruses, we propose to name VA2 as Malvastrum yellow vein virus.

根据&双生病毒科病毒全基因组核苷酸序列同源性小于89％，往往定名为不同病毒；大于89％，则认为是同一病毒的不同株系&这一原则，VA2为双生病毒的一种新种，根据病毒分类委员会命名原则，我们将该病毒命名为赛葵黄脉病毒，英文名为Malvastrum yellow vein virus，简称MYVV。

RbcL gene was obtained by polymerase chain reaction and cloned into pDM19-T vector. The positive clone identified by PCR was sequenced. The nucleotide sequence was 1266 bp long, including an ORF of 1031bp, encoding a putative 343 amino acids. The deduced amino acid sequence had a high identity above 94.13% with that of tobacco, spinach, sweet potato, corn, Arabidopsis thaliana, Oryza sativa, Vitis vinifera, Marchantia polymorpha and Citrus paradise.

阳性克隆鉴定后进行测序，序列分析结果表明：该基因片断长为1266 bp，包括1031 bp的编码区序列，编码343个氨基酸；其编码区氨基酸与烟草、菠菜、玉米、甘薯、拟南芥、水稻、葡萄、地钱和葡萄柚等9个物种的同源性94.13%以上，并构建了它们间的亲缘关系树。

A shot-gun DNA sequence strategy was employed,in which the mitochondrial genome library of Muntiacus reevesi has been constructed to obtain the complete mitochondrial genome sequence.

通过建立麂属动物小麂线粒体DNA文库、鸟枪法测序，获得了小麂线粒体基因组全序列并对其基因组成、蛋白质的编码序列、tRNA基因等结构作了详细分析，这也是国内有关哺乳动物线粒体基因组全序列的首次报道。

AY452490, AY452491, AY452492, AY452493, AY452494 and AY452495, respectively. Based on the analysis of nucleotide sequence homology in phylogenetic rRNA repeating gene family sequences from Siniperca chuatsi, human, Norway Rat, Tetraoden nigroviridis, oreochromis esculentus, cyprinus carpio and oncorhynchus mykiss, the pairwise nucleotide sequence homology of 18S, 5.8S, 28S, ITS1 and ITS2 among 7 organisms were separately showed to be 91.2-98.6%, 95.0-100%, 87.2-92.8%, 6.8-62.9% and

同时，将鳜鱼rRNA重复基因家族序列与人、挪威鼠、鲀鱼、罗非鱼、镜鲤和虹鳟之间的序列分别进行同源性与进化关系分析，发现18S、5.8S、28S、ITS1和ITS2的同源性分别在91.2 98.6％、95.0 100％、87.2 92.8％、4.9 62.9％和11.9 64.0％之间；并获得了它们之间的进化关系树。

At last, based on the wavelet transform of logging signals, the stratigraphic sequence correlation and analysis of the coalbearing strata of Permo-Carboniferous in Luxi and Jiyang area have been done. The stratigraphic sequence framework of main coal-bearing strata formed in Neopaleozoic in Shandong area is established.

最后在测井信号小波变换的基础上对鲁西和济阳凹陷的含煤地层进行了层序对比和分析，基本建立了山东地区晚古生代主要含煤地层的层序地层格架。

Based on the date of outcrop, log and lithology, sequence stratigraphic division and correlation were made on Neopaleozoic in south of North China Basin, and high-precision geologic correlation framework was built up in the basin.2. In the term of sedimentary features of Neopaleozoic in south of North China Basin, lateral variation of sedimentary facies was studied selectively in Benxi-Taiyuan Formation, Shanxi Formation, Lower Shihezi Formation, Upper Shihezi Formation and Shiqianfeng Formation. Lithofacies paleographic features and the sedimentary evolutionary process of Late Paleozoic in south of North China Basin were worked also.3. On the basis of sand-bodies Isopach figure drawing, the distribution of major reservoir sand-bodies of Neopaleozoic in south of North China Basin was studied. Basing on the distribution of sand-bodies in sequence stratigraphic framework, combining the development and distribution features of barrier and underwater distributary channel, there is a disquisition on the distribution regularity of pay reservoir sand-bodies of Neopaleozoic in this area.

以露头、测井和岩性资料为基础，对华北盆地南部上古生界进行了层序地层的划分与对比工作，建立了盆地范围内的高精度地层对比格架。2、根据华北盆地南部上古生界的沉积特点，按本溪组—太原组、山西组、下石盒子组、上石盒子组和石千峰组5个层组分别开展了沉积相的横向变化对比研究，并以此为基础探讨了华北盆地南部晚古生代的岩相古地理特征及其沉积演化过程。3、以编制砂体等厚图的方式研究了华北盆地南部上古生界主要储集砂体的平面展布规律，并以层序地层格架下的砂体展布研究为基础，从沉积体系控制下的砂体分布特点出发，结合障壁岛与水下分流河道的发育与分布特点，系统深入地开展了区内上古生界有利储集砂体的分布规律研究。

Bioinformatics analysis showed that the gene sequence was highly consistent with previously reported F3,5H genes, such as GenBank Accession D14588, Z22545, and DQ352142. The encoded amino acid sequence was highly homology with that of GenBank Accession BAA03438.1, BAC10997.1 (Nierembergia sp. NB17) and AV85470.1 at 99%, 88% and 87%, respectively.

生物信息学分析表明克隆的F3，5H基因与GenBank登记号D14588、Z22545和DQ352142等的F3，5H基因序列高度一致；其编码的氨基酸序列与GenBank登记号BAA03438.1、BAC10997.1和AAV85470.1的高度同源，同源率分别为99%、88%和87%。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷