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Strains YL001 and YL002 formed a monophyletic clade with strains of X. nematophilus with sequence homology outweighing 99% and the sequence homology to genus Photorhabdus outweighing 94%. The lag, logarithmic, stationary and contabescence phase of the two bacteria were 0-6, 6-18, 18-66 and 66 h respectively; pH of strains YL001 and YL002 reduced to 5.70 and 5.56 at 12 h, respectively, and then rose gradually to 7.74 and 8.07 respectively when culture finished. Glucose content reduced quickly during 0-18 h, and then kept stable. Amido nitrogen content reached the lowest at 12 h and then rose slowly. YL001 exhibited the highest inhibitory effects at 54 h and YL002 exhibited highest inhibitory effects at 42 and 66 h respectively on B. cirerea and B. subtilis. Strains YL001 and YL002 belonged to X.

YL001和YL002菌株与嗜线虫致病杆菌种内菌株形成一个类群，序列同源性大于99%，与发光杆菌属内菌株的序列同源性大于94%。2菌株的延缓期、对数生长期、稳定期和衰亡期分别为0~6，6~18，18~66和66 h；培养12 h后，YL001和YL002菌株发酵液的pH值分别降低至5.70和5.56，此后逐渐上升，至发酵结束时其pH值分别为7.74和8.07；培养0~18 h时2菌株发酵液中还原糖含量迅速降低，此后保持稳定；12 h时氨基氮含量达到最低，此后开始缓慢上升；YL001菌株培养54 h后及YL002菌株培养42和66 h后，其对番茄灰霉病菌和枯草芽孢杆菌的抑制作用最强。

Japonicus used in this experimentation is matemal inheritance.5 Sequence analysis of mitochondrial 16S rRNA and COI gene and molecular phylogeny of four species of sea cucumber500 bp 16S rRNA and 540 bp COI fragment were obtained by PCR method from mitochondrial DNA of four species of sea cucumber, Apostichopus japonicus, Holothuria fuscogilva, Cucumaria frondosa and Mensamaria intercedens. Variable sites, nucleotide diversity, average number of nucleotide differences and average number of nucleotide substitutions per site were calculated to analyze sequence difference, and phylogenetic trees were constructed by the NJ and ME method.

结果显示在来自这5组家系的100个后代中仅检测到母本的单倍型，刺参的线粒体遗传模式表现为典型的母系遗传。5 4种海参16S rRNA和COI基因片段序列比较及系统学研究采用PCR技术对山东荣成、长岛、俄罗斯和日本的刺参，黄乳海参，北大西洋瓜参，二色桌片参的16S rRNA和COI基因片段进行了扩增和测序，分别得到了长度约为500 bp和540 bp的片段。

After the multiple sequence alignments, the sequence variation analyses were conducted and the molecular phylogenetic trees of Cypraeidae were reconstructed with NJ, MP and bayesian methods, using Prosimnia semperi of Cypraeacea as an outgroup.

其次，用自测的宝贝科11种软体动物的mtDNA COI基因序列，结合得自GenBank上的18种宝贝科软体动物的同源序列，用NJ法、ML法以及贝叶斯法构建了分子系统树。

Full-length cDNA sequence of NPY from Barbodes sinensis Based on multiple alignment of a number of Cyprinidae NPY genes, two special primers of 3'cDNA ends were designed for amplification of the 3'cDNA ends of NPY genes and the sequence was acquired from the cDNA templet of the brain of S.sinensis.

中华倒刺鲃基因推导氨基酸序列的分析中华倒刺鲃NPY的cDNA编码区核苷酸长度为291bp，编码96个氨基酸，由信号肽(28个氨基酸)、NPY成熟肽(36个氨基酸)、三肽加工位点(3个氨基酸)和连接肽(29个氨基酸)组成。

The results of 16S rRNA sequence and phylogenetic analysis show that the strain is the closest relative to Desulfovibrio sp. with 96.0% sequence similarity.

基于16SrDNA序列同源性构建了系统发育树，结果表明此株菌是属于脱硫弧菌属的硫酸盐还原菌，与Desulfovibrio具有96.0%的序列相似性。

The sequence of Diurnal change of WUEph of different plants in lawn community is: Geranium sibiricum, Leymus secalinus, Potentilla supine., Viola prionantha, Poa pratensis and Potentilla anserine:The sequence of physiological drought resistance of weeds and turf in lawn community is: Poa pratensis, Digitaria ischaemumjotentilla supine, Potentilla anserine, Viola prionantha, Geranium sibiricum and Inula britanica.

草坪群落中不同植物的水分利用效率日变化均值大小为：鼠掌老鹳草＞赖草＞铺地委陵菜＞早开堇菜＞草地早熟禾＞鹅绒委陵菜。

Methods:64 cases of intraspinal spacc-occupying discase were examined by conventional myelography,CT myelography and MRI,CTM were performed after 6 hours of myelography,MRI were performed with SE sequence and combined with GE sequence.

脊髓造影采用常规脊髓造影和 CTM相结合。CTM在常规脊髓造影后 4～ 6 h进行。 MRI采用 SE序列加 GE序列。椎管内占位性病变的病灶检出率、定位和定性准确率做一统计和分析。

The PPDK gene had been cloned from many plants. The research on its protein structure has not been studied. This paper analyses Echinochloa crusgalli var frumentaceaW.F.Wight's PPDK sequence by bioinformatics and model its three-dimension structure.Sequence analysis of Pyruvate orthophosphate dikinase from was conducted by bioinformatics tools such as DNAstar,Vector NTI and SignalP etc.

目前已从许多种植物中克隆了其PPDK基因，但是对于其蛋白结构的分析尚未报道，此文通过生物信息学方法对家稗PPDK的序列进行了分析，并对其三维结构进行了建模分析；利用DNASTAR、Vector NTI 9、SignalP等生物信息学平台对Echinochloa crusgalli var frumentaceaRoxb。

The effects of the relation on recording geometry with effective electrooptical coefficient and space charge field, wavelength of diffraction beam and diffraction angle on exposure sequence are taken into account, and the perfect exposure sequence is discussed based on recurrence method.

考虑有效电光系数和空间电荷场随写入光路几何组态的变化关系，以及各信道衍射光波长、衍射角等因素对曝光时序的影响，利用递推法推导出更为精确的曝光时序。

The sequence of the causes of death in male was circulatory system diseases,malignant neoplasms,respiratory diseases,accidents and digestive system diseases.The sequence in female was the circulatory diseases,malignant neoplasms,respiratory diseases,the endocrinological diseases and accidents.

男性死亡率死因顺位依次为：循环系、肿瘤、呼吸系、损伤和中毒及消化系疾病；女性死亡率死因顺位依次为：循环系、肿瘤、呼吸系、内分泌及损伤和中毒。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷