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A series of methods and software programmes for generating truncated Gold sequence are brought forward in this paper, the correlation and balance characteristics of different truncated proportion Gold sequence have been analysed through the simulation of truncated Gold sequence performance, involve with before and after truncated, different period of sequence and different truncated length.

在本文中，研究了截短平衡Gold序列的产生方法，给出了生成截短平衡Gold序列软件程序，通过对截短前后、不同序列周期、不同截短长度的截短Gold序列的性能仿真，分析了不同截短比例Gold序列的相关特性和平衡特性。

From bottom to top, a complete terrigeous sequence in fault-depressed lacustrine basin consists of the following sequence units in turn: Sequence Boundary→Lowstand System Tract (no coast-onlap)→Trangressive System Tract (distinct coast-onlap)→Highstand System Tract being composed aggradational lacustrine mudstone with low sedimentation rate→Regressive System Tract (distinct coast-dropback)→Sequence Boundary.

断陷湖盆中一个完整的陆相层序自下而上依次由下列层序单元构成；层序界面→无湖岸上超的低水位体系域→湖岸显著上超的水进体系域→由沉积速率缓慢的垂向加积型湖相泥质岩构成的高水位体系域→湖岸显著退却的水退体系域→层序界面。

After two-cluster testing,branch length testing and relative rate testing ,two sequences(Melantitta nigra and Aythya americana) of 15 Cty b sequences were deleted because thek evolutionary rate were heterogeneous. The divergence between Anseriformes and Galliformes was chosen as an anchor point. Nucleotide sequence and amino acid sequence of 13 sequences data set was used to calibrated Anatidae molecular clocks. The results indicated that amino acid sequence is better than nucleotide sequence when divergence time is calculated with two kinds of sequences. The reliable divergence time of Anatidae were estimated with """"1+2TiTv"""" model.

经过二簇检验、分枝长度检验和相对速率检验，删除2条速率不恒定的序列后，用剩余的13条序列，以鸡形目与雁形目的分歧时间(89.8MYA)为锚定点，用顺序标定法标定鸭科鸟类分子钟的分析结果表明，用&1+2TiTv&分析得到的鸭科鸟类的分歧时间比较可靠；氨基酸序列用泊松校正模型和Γ模型估计的分类单元间的分歧时间则比较一致，表明氨基酸序列更适合用于鸭科鸟类分子钟的研究；用同一基因标定分子钟时，较长序列得到的结果标准误比较小；与核苷酸序列相比，氨基酸序列更适于用作分子钟的标定。

The cDNA document of ACC oxidase gene of Citrus aurantium L. was cloned. By using the program of BLAST on NCBI GenBank database, the sequence presented a very high match with the ACC oxidase genes from other plants. The base sequence was analysed by using biology programe of DNAStar 5.0. 278 amino acids were coded by the base sequence and the base sequence had the same conserve region of ACC oxidase gene of many kinds of other plants. The base sequences comparability was more than 72% compared with those of many kinds of other plants. The amino acid sequences comparability was more than 70% compared with those of a lot of other plants.

克隆了酸橙1-氨基环丙烷－1-羟酸氧化酶基因cDNA片段，将片段序列在NCBI网站上进行同源性搜索，显示的皆为不同植物的ACC氧化酶基因，因而认为所克隆的片段就是酸橙ACC氧化酶基因；并运用DNAStar5.0软件进行序列分析，推导的氨基酸序列为278个残基；具有所有植物ACC氧化酶基因共有的保守区域；与多种植物的ACC氧化酶基因的核苷酸和氨基酸序列的同源性都在72%和70%以上。

Some new concepts and algorithms are proposed,such as jobnomial sequence,job perturbation sequence,neighbourhood of job nomial sequence and jobsequence perturbation analysis.These job sequence PA algorithms extend PA techniques aboutcontinuous parameters to discrete parameters and provide some new kinds of tools for solving suchcombination optimization problem.

论文提出了工件序的邻域、摄动序等概念以及对工件序进行摄动分析的思想和算法，将传统的PA算法从连续参数领域推广到离散参数的组合优化领域，为此类组合优化问题增添了新的求解工具。

First 46 viral sequence's PA fragments in year 2009 are analyzed. Then the siRNA target genes with strong ability of interference are selected on the basis of the secondary structure of siRNA target sequence. Our research reveals that the outbreaking H1N1 virus in 2009 is characterized by steady heredity, high sequence conservativeness, uniform siRNA target gene sequence, and high conservative structure.

对2009年的46株病毒序列的PA片段进行分析，从经过序列分析所获得的众多靶系列中，采用结构分析手段对靶序列进行筛选，获得较易干扰的靶序列及设计出相应的siRNA。

Basing on theregional sequence stratal framework, carry through the elaboratecompartmentalization of sequence stratum in major horizon and basing on seismicand logging data, use seismic sequence stratigraphy and drilling sequence stratigraphyto discuss isochronous stratal distributed pattern in framework.

在建立区域层序地层格架的基础之上，对重点层位进行精细层序地层划分，在地震资料和测井资料的基础上，利用地震层序地层和钻井层序地层，探讨等时地层格架内的地层分布模式。

Highprecision sequence stratigraphy was applied to divide and correlate the different sequence stratigraphical units from the Upper Cretaceous to plaeogene in the Biyang depression, and a sequence stratigraphic framework which can correlate the different sequence stratigraphical units was set up to point out that the ramp region of the northdepression is an oil and gas reservoir featured by altitude progradational delta and complex fault blocks, the inner belt of the north slope hinge zone is a low order faultlithologic oil and gas reservoir, the development stages and high order terms of the deep depressed area is a fluxoturbidite and sublacustrine fan lens trap, and the south actic region is a lithologic hydrocarbon reservoir development area.

运用高精度层序地层学的理论，系统地划分和对比泌阳凹陷上白垩统至古近系不同级别的层序地层单元，建立了凹陷内各级层序地层单元的对比框架，指出凹陷北部斜坡带为高位进积三角洲复杂断块群油气藏，北部斜坡枢纽带内带为低位扇断层-岩性油气藏、深凹区湖扩展期和高位期为滑塌浊积体及湖底扇透镜体圈闭，南部陡坡带为岩性油气藏发育区。

We amied the BCP sequence and the preS2 gene sequence of HBV separately as the target region, according to HBV DNA sequence of Chinese strain, synthesized the set of specific primers, amplified the sequence by PCR method from the serum of patients with CHI, verified by restriction analysis, subcloned into pGEM Teasy vectors, employed polyacrylamide gel electrophoresis to display the deletion mutation and selected the clones with differential length to be sequenced.

本研究应用PCR技术及其他分子生物学技术，分别以HBV转录调控序列BCP以及表面蛋白编码序列pre S2为靶基因，以中国株HBV基因序列为依据，设计特异性多聚酶链反应引物，分别自43例、51例慢性HBV感染患者血清中扩增出目的片段，构建质粒Teasy-BCP和Teasy-pre S2，酶切鉴定后，采用聚丙烯酰胺凝胶电泳技术展示缺失突变，再经DNA测序以了解在慢性乙型肝炎患者体内的病毒变异情况。

The nucleotides sequence and deduced amino acid sequence of these cDNA figments mentioned above were divided into two fractions. One is carbon part of L gene , the other is 5 terminal,and they were compared with other rabies-related virus strains published in NCBI previously.The result show that the homology of nucleotide sequence and amino acid sequence of carbon part in L gene among these two rabies virus strains is 91.3% and 95.8%, and these two strains belong to type of gene I.

本实验以吉林鹿源DRV8202株及河南鼠源野毒株MRV的L基因的C端为主要研究内容，用RT-PCR和3'-RACE方法对其进行了克隆和测序，将所测得的核苷酸序列和推导的氨基酸序列分成两个部分，一是L基因C端序列，另一个是L基因5'端的非编码区，并与NCBI上公开发表的狂犬病毒其它毒株的相应片段进行分析比较。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。