查询词典 sequence

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

Key words: Boophilus microplus;suppression subtractive hybridization;expressed sequence tag;sequence analysis

微小牛蜱；抑制消减杂交；表达序列标签；序列分析

The intergeneric phylogenetic trees of water lilies showed that Nelumbo and Ceratophyllum form a sister group, but with relatively high sequence divergence, supporting that place the two genera into two distinct families; and Brasenia and Cabomba form a sister group, with relatively low sequence divergence, supporting that the two genera were placed the same family Cabombaceae.

结果表明：莲属与金鱼藻属构成姐妹群，但序列分歧度较大，支持将两个属独立成科的观点；莼菜属与水盾草属构成姐妹群，且序列分歧度较小，支持现有的莼菜科处理。

Sequence analysis of this Full-length pax-6 cDNA fragment of Bufo raddei Strauch showed a 2026-bp sequence.

这是一个在进化上相当保守的基因，说明了该基因在眼睛和晶状体发育过程中的重要作用和在进化过程中的重要地位。

Roots of five species of Calanthe and two species of Phaius from north, midland and south of Taiwan were collected for the isolation of orchid mycorrhizal fungi. Total for 104 isolates of fungi were isolated. They are identified respectively by the traditional method or DNA sequence. Results showed thatTrichoderma and Mucor were most often isolated fungi. The DNA sequence results showed that only Xylaris was most common fungus.

由台湾北、中、南四处地方采集五种原生根节兰及二种原生鹤顶兰，进行此等原生兰花根部菌类分离，共分离出104株代码真菌菌种，分别再以菌落、菌丝和孢子等传统形态特徵鉴定菌种，结果发现以木霉菌及毛霉菌属内之菌株占优势，而利用核酸序列比对之鉴定结果则以炭角菌属内之菌株占多数。

The cDNA open reading frame of IFN-γis 501bp,encoding a putative 166 amino acid protein (19.327KD), the DNA sepuence homology of thiscaprine IFN-γand the corresponding caprine(U34232) cytokine is 99.4％, SignalP 3.0 analysisdemonstrated that the first 23 amino terminal aa sequence compose a hydrophobic signal sequence.

测序结果显示克隆的IFN-γ基因全长501个核苷酸，编码166个氨基酸，该基因与GenBank发表的山羊干扰素-γ基因序列(U34232)比较，核苷酸／氨基酸序列同源性为99.4％。

The IL-2 gene is 468bp, encoding a putative 155 amino acid protein, the DNA sepuencehomology of this caprine IL-2 and the corresponding caprine(AF535145) cytokine is 100.0％, thegene homology to Qihua Yings is 99.1％, the first 20 amino terminal aa sequence compose ahydrophobic signal sequence.

经SignalP 3.0分析表明，前23个氨基酸为信号肽序列；IL-2基因开放阅读框架共有468bp，编码一条155个氨基酸的多肽，与GenBank发表的山羊白细胞介素-2基因序列(AF535145)比较核苷酸／氨基酸同源性为100.0％，与应琦华等的山羊IL-2序列的同源性为99.1％，前20个氨基酸为该蛋白的信号肽序列。

Molecular beacon coupled with carminic acid was electrochemical inactivity,but after hybridized with complementary sequence, electrochemistry signal was greatly enhanced,and it was linear related with the concentration of complementary sequence in the range of 6.0×10~(-8)mol/L～1.4×10~(-6) mol/L,with the detective limit of 1×10~(-8)mol/L.

此类分子信标本身在实验选定条件下无电化学信号，与完全互补序列杂交后产生电化学信号，论文采用碳纳米管修饰电极对杂交前后的电化学信号进行检测。

A pair of specific primers was designed according to the reported cDNA sequence of the DC-ACO(Dianthus caryophyllus L.ACC Oxidase),and a partial sequence of the gene was amplified from genomic DNA of a carnation variety 'Master' by PCR.

据已报道的香石竹氨基环丙烷羧酸氧化酶基因的cDNA序列设计特异引物，以香石竹品种&Master&基因组DNA为模板，用PCR方法克隆出香石竹ACO基因的部分序列。

The C18 known sequence, whose sequence had been known but function remained unknown, was expressed in cataractous lens epithelium but was not expressed in normal lens epithelium.

序列已知而功能未知的C18已知序列在白内障晶状体上皮有表达，但其在正常晶状体上皮中无表达。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。