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The results showed that RSAP used two primers of 18 nucleotides in which starting at the 3' end of each primer were restriction site sequences (4-6 bases),followed 12-14 bases of arbitary sequence, the differences between two primers were restriction sites and filler sequence; PCR am-plification was run for the first 5 cycles with an annealing temperature of 35℃, followed by 35 cycles with an annealing temperature of 48℃; the PCR reaction of 25 μL included 20ng DNA templates, 2.5mmol/L of Mg(superscript 2+), 0.2 mmol/L of dNTP, 1.5U of Taq DNA polymerase, 600nmol/L of each primers. RSAP is a new maker technique with simplicity, moderate throughput ratio and reliability. RSAP is of good reprodicibity and broad application.

结果显示,RSAP技术的引物为2条长度均为18bp的引物,引物的3'端为4~6个碱基的限制性酶切位点序列,接着是12~14个碱基的随机序列,2条引物的限制性位点和随机序列不同:PCR扩增的前5个循环采用35℃的退火温度,随后的35个循环采用48℃的退火温度;在25μL反应体系中,模板DNA用量为20ng,Mg(上标 2+)浓度为2.5mmol/L dNIPs浓度为0.2 mmol/L,Tap DNA聚合酶用碳为1.5U,2条引物浓度均为600mmol/L;RSAP技术重复性好,适用性广泛,是一种操作简便、产率中等、稳定可靠的DNA标记技术。

In a time-invariant system there will be a shift in the output sequence of numbers for a delay applied in the input sequence.

在一个时间不变式的系统将有转移在数字中产品序列被申请的延迟的 in输入序列。

Additionally, this thesis also analyzes the factors which can influence the properties of chaotic sequence and points out the merits of the new presented chaotic sequence.

此外,本文还进一步讨论了影响混沌序列性能的一些因素,验证了文中提出的新的混沌序列所具有的优点。

In particular, we give an algorithm for computing the FCM of a Sturmian sequence and study further the property of FCMs of the ThueMorse sequence and the fixed points of invertible substitutions.

在第一章中,我们引入了因子组合矩阵以研究序列的组合性质。

This paper discusses convergence of Ishikawa iteration sequence and existence of fixed points for set-valued nonexpansive mapping in uniformly covex Banach space, and the conditions are shown which guarantee the convergence of the iteration sequence to a fixed point.

讨论了δ集值非扩张映象在一致凸Banach空间中不动点非空的充分必要条件与Ishikawa迭代序列的收敛性及确保迭代程序收敛到不动点的条件,所得结果是单值非扩张映象的推广和发展。

Using the known results on CCF of m-sequences, the parameters of LCZ sequence sets obtained from composite GMW sequences are analyzed and then two classes of asymptotically optimal binary and multiphase LCZ sequence sets are obtained.

利用m-序列互相关特性的已知结果,论文比较分析了由GMW序列的复合序列得到的具有低相关区的序列集的参数,得到了两类渐近最佳的二元和多相序列集。

Best scheduling is obtained by searching good sequence in the sequence space.

在序列空间中,通过寻找好的序列以得到最佳的排序。

By using proper interweaves, we can increase free space of output sequence when we don' t change code of input sequence.

通过适当的交织器,可以在不改变Turbo码输入序列码重的情况下增加输出序列的自由距。

A new generating circuit on five-level M-sequence based on the cut-join sieve method,which can generate 2048 different five-level M-sequence s.

敌我识别系统的核心技术之一是询问信号与应答信号的加密传输,针对信号传输中对密码的要求,文中引入了伪随机码,基于剪接筛法设计了能产生2048个两两相异的5级 M序列的电路,并对该电路进行了仿真,仿真结果验证了该设计的正确有效性

And 0.64±0.05GPa for sillimanite zone, 750~800℃ and 0.9~1. 0GPa for partial melting migmatites. The results generally show that the kyanite sequence metamorphic belt is a normal medium-pressure sequence.

十字石带有后期向低压转变的趋势;矽线石带的变质条件比中压型正常正常演化条件低,这可能是保留了后期向低压转变的特点。

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相关中文对照歌词
Sequence 1
Sequence 2
Sequence 4
Sequence 5
Sequence 6
Sequence 7
Emerald City Sequence
Heartland (Main Title Sequence)
Breathing In Sequence
Initialization Sequence
推荐网络例句

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是,在否定句或疑问句里,这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷

Nusa lembongan : Bali's sister island, coral and sand beaches, crystal clear water, surfing.

Nusa Dua :豪华度假村,冲浪和潜水,沙滩,水晶般晶莹剔透的水,网络冲浪。