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ARID (AT-rich interaction domain) protein is a transcription factor family in higher eukaryotes that regulates cell proliferation, development, and differentiation. Specificity of DNA binding ability in this family prefers AT-rich sequences, but some ARID family proteins are not sequence-specific DNA-binding proteins or they do not bind AT-rich sequences. We found two genes that encode ARID in Giardia lamblia genome database, garid1 and garid2. We analyzed the function of garid1 first. AU1-tagged gARID1 was found to localize to nuclei. During encystation, gARID1 mRNA level decreased emphatically, but protein level increased. We also found that gARID1 can bind AT-rich initiator of the cwp1 promoter by EMSA. Mutation analysis revealed gARID1 binding sequence was AGATC and AATAAAATA. We used ChIP to demonstrate that gARID1 can bind cwp1 gene promoter in vivo.

ARID(AT-rich interaction domain)蛋白质家族是真核生物的一种转因子，在许多同种的真核生物有它的同源基因，这个家族的蛋白质通常与调控细胞的生长、发育和分化的作用有关，而这个家族的蛋白质和DNA的结合能，各种ARID蛋白质的专一性尽相同，过大致上偏好於和AT-rich的序结合；我们已经在形鞭毛虫的基因组中找到个含有ARID 的基因，分别是garid1和garid2，我们首先对於garid1做分析；将AU1标记接到gARID1转染形鞭毛虫，用免疫萤光染色可发现gARID1存在於细胞核中。gARID1的讯息RNA在囊体化后会明显下，过其阳性染色和蛋白质表现有明显增加；EMSA实验中也发现gARID1会明显的与cwp1基因启动子之AT-rich initiator结合，经由突变序分析，也显示gARID1的结合序为AGATC和AATAAAATA，随后我们也用ChIP证明gARID1在细胞内也的确会和cwp1基因的启动子结合。

The invention provides a method for achieving priority level for generating sequence of mapping items which are transformed by network address, the method comprises the following method, first, when an address conversion apparatus needs to converse address after the address conversion apparatus receives a data packet, judging whether the items are generated, upwards sending the data packet to a CPU when a mapping item is not generated, step B, judging whether concurrent mapping number exceeds the preset mapping enqueue threshold with the CPU, if exceeds the preset mapping enqueue threshold, and judging whether a Referer field is contained in HTTP data, and recording the Referer field or a GET field according to judgment result, writing in a REF table, and arraying a priority level for each user according to a write sequence, and step C, leading the CPU to generate map in turn according to the priority level in the REF table, and then sending down to a forwarding layer to transfer a data packet.

本发明提供一种对网络地址转换的映射条目生成顺序实现优先级的方法，包括下列步骤：步骤A：当地址转换设备收到数据包后，需要进行地址转换时，判断映射条目是否已经生成，在未生成映射条目时，将数据包上送至CPU；步骤B：CPU判断并发映射数是否超过预定的映射入队门限，如果是，则判断在HTTP数据中是否含有Referer字段，并根据判断结果记录Referer字段或者GET字段，写入REF表中，并将每个用户依照写入顺序排列出优先级；步骤C：CPU依照REF表中的优先级依次生成映射，然后下发到转发层面转发数据包。

In comparison to the vector containing 4-exa gene without spacer peptide, the expression level of vectors containing glycosylation site sequence and enterokinase recognition site sequence as spacer was increased by 4 and 9 times, respectively.

在EXA的N端增加间隔肽可促进蛋白分泌，与不含间隔肽序列的4拷贝EXA串联表达载体相比，含有糖基化位点和肠激酶识别位点间隔肽的插入，使蛋白表达水平分别提高了4倍和9倍。

The objective of the study was to obtain the gene of bovine enterokinase light chain,which would be used in the cleavage and purification of fusion proteins.The fragment of bovine enterokinase light chain cDNA was obtained by RT-PCR from a sold bovine′s duodenal mucosa, then cloned into the pUCmT cloning vector and sequenced.Compared with the sequence deposited in GenBank,the cloned gene sequence is correct.

为克隆表达牛肠激酶轻链编码基因，以期应用于融合蛋白的切割与纯化，从市售北方黄牛十二指肠组织中提取总RNA，以RT-PCR方法扩增其cDNA片段，将此片段克隆于pUCmT载体中，经过特异性限制性内切酶酶切分析片段正确后，进行全序列分析。

Based on the sedimentary combination, lithology and sequence characteristic, two classes of sequence structures were generalized as structure entireness and structure lackness.

并根据剖面纵向沉积组合、岩性特征及层序特征，概括出结构完全型和结构缺失型两类层序结构。

The most common approach is to consider the problem of machine equivalence which defines two synchronous design specifications functionally equivalent if, clock by clock, they produce exactly the same sequence of output signals for any valid sequence of input signals.

最常用的方法是考虑的问题，机等价定义两个同步设计规格等同功能，如时钟的时钟，它们产生完全相同的序列输出信号的任何有效的序列输入信号。

Chapter 3, in the above system, gives out the definitions of topological new transitivity, topological transitivity, topological strong transitivity and topological conjugate of a sequence of maps, studies basic properties of the above topological conjugate, and obtains some main results, proves topological new transitivity and topological transitivity are equivalent u-under a compact metric space, a sequence of full maps and interchangeable with each other, and that some conclusions associated with topological conjugate.

第三章，在上述系统中给出了一列映射的拓扑新传递、拓扑传递、拓扑强传递和拓扑共轭的定义；研究了一列映射的拓扑共轭的基本性质，得到了一些主要结果；证明了在底空间是紧致度量空间、一列映射是满映射并且两两可交换的条件下拓扑新传递和拓扑传递是等价的；还证明了几个与拓扑共轭相关的结论。

On the basis of analyzing the characteristic of the basic immune algorithm and utilizing the ergodic property of the chaotic sequence, an adaptive immune evolutionary algorithm combined with chaotic sequence is proposed and used at the training of the neural networks.

在分析基本免疫算法优化性能的基础上，利用混沌序列的遍历性，给出了一种嵌入混沌序列的自适应免疫进化算法，并将其应用于神经网络的训练中。

The common denominator of both is 20. 1 applied the Fibonacci sequence to the number 20 and carried the sequence out to 26 places.

两者的共同数字是20.1应用在数目为20的斐波纳契序列上，把序列携带到26处空间。

Niu_jiantao的正确 The Fibonacci sequence 1,1,2,3,5,8,13,21……starts with two 1s,and each term afterwards is the sum of its two predecessors,which one of the ten digits is the last to appear in the units position of a number in the Fibonacci sequence?

Fibonacci数列1，1，2，3，5，8，13，21……以两个1开头，此后每一个数都是前两个数的和。那么在Fibonacci数列中，10个阿拉伯数字中的那一个是在个位数上最后出现的？

They weren't aggressive, but I yelled and threw a rock in their direction to get them off the trail and away from me, just in case.

他们没有侵略性，但我大喊，并在他们的方向扔石头让他们过的线索，远离我，以防万一。

In slot 2 in your bag put wrapping paper, quantity does not matter in this case.

在你的书包里槽2把包装纸、数量无关紧要。