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Secondly, the genomic DNA is extracted from Zostera marina L. and then amplified by PCR with two oligonucleotide primers synthesized to obtain the partial nucleotide sequences of the Na~+/H~+ antiporter gene. The Na~+/H~+ antiporter gene clone was obtained after a series of manipulation: purificatioin of PCR product, ligation and transformation of recombinant plasmids, blue-white selection and so on. DNA sequence analysis shows that its DNA sequence is highly homologous with the genomic DNA sequence of E.

其次，通过提取大叶藻基因组DNA，根据设计的简并性引物进行PCR扩增，纯化PCR产物，连接，重组质粒转化，蓝白斑筛选等操作，并测序，提交测定序列到NCBI进行BLASTN，结果表明：该序列与大肠杆菌的基因组序列相似性较高，而与其它物种的Na~+/H~+逆向转运蛋白基因没有相似性。

In this study,one pair of specific primer for mature chicken interleukin-18(ChIL-18) cDNA was designed and synthesized according to the previously published gene sequence of ChIL-18.The full length cDNA gene of ChIL-18 encoding mature active protein was amplified from LPS–stimulated MDCC-MSB1 cells by Reverse Transcription-Polymerase Chain Reaction.Then it was cloned into pMD18-T vector. Sequencing analysis showed that the nucleotide sequence of this ChIL-18 mature protein gene was 5l0bp including the stop coden and the same as the published ChIL-18 cDNA sequence by Schneider K.

本研究根据已发表的ChIL-18成熟蛋白(mature ChIL-18，mChIL-18)的cDNA基因序列，设计一对特异性引物，应用反转录-聚合酶链式反应(reverse transcription-polymerase chain reaction，RT-PCR)技术从脂多糖刺激10小时活化的马立克氏病成淋巴细胞样细胞系MDCC-MSB1的cDNA中扩增出编码鸡IL-18成熟活性蛋白的全长基因，对扩增片段进行T-A克隆，经PCR、酶切鉴定及测序验证，成功获得ChIL-18成熟蛋白完整基因的克隆。

Amino acid similarities between goat and human were 87% and between gaot and mouse were 83%. Just like other members of FGFs superfamily, FGF5 protein also has a signal sequence ,at same time its protein has higly similar sequence about 120 Amino acid in central core sequence with other breed. Tissue specific expression of the FGF5 gene was also investigated in cashmere goat by RT-PCR analysis. The results showed that FGF5 gene was expressed in different time of the tested skin tissues, including anage and telegon.

结果如下：(1)本研究利用 RT-PCR 方法克隆了羊 FGF5 基因的 cDNA 序列，得到了 925bp的序列，并对该序列进行了分析，与成纤维细胞生长因子家族的成员一样也是由三个外显子组成，也具有一信号序列，成熟蛋白为 270 个氨基酸，经同源性比对得出大鼠与小鼠的 FGF5 氨基酸同源性最高为 97%，在与羊的蛋白质比较中大鼠与羊的同源性为 83%，小鼠与羊的同源性为 84%，人与羊的同源性最高，为 89%。

Based on symmetrical analysis theory, the stator current of motor is decompounded into positive-sequence current, negative sequence current and zero-sequence current to distinguish the running state and fault type of the motor according to their magnitude of current. The technology of INTEL80C196KB MCU is applied to design and fulfill a new kind of micro-processor protective device for asynchronous motor.

根据对称分量分析原理，将电动机定子电流分解成正序、负序和零序分量，依据各种序量的大小，判别电动机运行状态和各种故障类型；采用INTEL80C196KB单片机技术，实现了一种新型的异步电动机综合保护装置。

Complete genome sequences of 20 viruses in Bunyaviridae loaded from NCBI were analyzed by two biological softwares of DNAMAN and DNASTAR. The results indicated great variability in both nucleic acid sequence and protein structure between plant viruses and animal viruses:(1) only plant viruses could encored NSm in M genome;(2) the length of nucleic acid sequence and protein sequence was different;(3) GC content of nucleic acid of animal viruses was higher than plant viruses of that;(4) protein topology analysis by online software SMART discovered that there was significant difference for the structure of glycoproteins G(subscript nG between animal-infecting and plant-infecting viruses;(5) usually, protein of plant-infecting virus had more complicated structure, more low compositional complexity and had N-Signal peptides except INSV.

本文从NCBI数据库下载具有完整基因组序列的布尼亚科病毒的5个属20种病毒的序列，用生物学软件DNAman，DNAstar进行比对分析，发现布尼亚科病毒属中的植物病毒在核酸序列及蛋白结构上与动物病毒有很大差异：(1)只有植物病毒的M基因组能编码NSm运动蛋白；(2)植物病毒和动物病毒在核酸序列和蛋白质序列长度均有差异，表明该科病毒是进化速度较快的病毒；(3)植物病毒核酸序列的GC含量低于动物病毒；(4)通过SMART网络软件进行蛋白质拓扑结构分析发现植物病毒和动物病毒在糖蛋白GG的结构上存在显著差异；(5)植物病毒糖蛋白结构较为复杂，有较多的紊乱区域，除INSV外，其他病毒都具有N端信号肽。

A method for rewriting onto a disk medium capable of being rewritten a limited number of times, comprises the steps of: encoding as data blocks all programs to be recorded; writing any encoded program onto a track on the disk medium as a given sequence of encoded data blocks; and, precessing along the track when overwriting the given sequence of encoded data blocks with a subsequent sequence of encoded data blocks.

一种用于写入和重写到能够被重写仅仅有限次数的盘媒体上的按照本发明方案的方法，包括步骤：将要记录的所有节目编码为数据块；将任何编码的节目的至少一部分写入到所述盘媒体上的轨道上作为所述编码的数据块的给定的序列；和当以编码的数据块的后续序列重写所述的编码的数据块的给定的序列时，沿着所述轨道旋进。

Sequence analysis and homology searches of public data bases, however, revealed no published sequences significantly similar to the sequence of the fragment, precluding a predicition of the potential function of the sequence.

但DNA序列分析和数据库检索表明，该片段与迄今发表的核酸序列均不存在显著的同源性，因此尚无法预测其潜在的功能性。

One second-order stacked sequence usually consists of a retrograding stacking third-order sequence set and a prograding stacking third-order sequence set.

一个二级叠加层序内通常发育一个退积的三级层序组和一个进积的三级层序组。

Whenever a batter hits into a double play, the double play is constituted by a sequence of physical events. It does not follow, however, that we could redene "double play" in terms of precise sequences of physical motions. This is so despite the fact that, if a particular physical sequence constitutes a double play, any physically indiscernible sequence would constitute a double play as well. So being a double play "supervenes" on physical sequences.

无论何时，例如在散打中，一个击打得了两分，这个两分击打是被一连串物理事件所构成，它并不由此得到我们可以根据精确的连续的物理运动来重新定义&双分击打&，尽管事实是，如果一个特殊的物理连续构成了双分击打，但任何物理上难以区别的连续运动也会构成双分击打、（所以，是双分击打，附加在物理连续上。

Guided with the theory of high resolution sequence stratigraphy and based on previous research results,we constructed sequence stratigraphic scheme of Qingshankou Formation which was in front zone of the west slope in southern Songliao Basin,where six sequence boundary surfaces were identified and 5 mediumterm baselevel cycleswere divided.

在前人研究的基础上，应用高分辨层序地层学理论与研究方法，对松辽盆地南部西斜坡地区的青山口组地层进行了层序地层划分与对比工作，从中识别出6个具等时意义的层序界面，将其划分为5个中期基准面旋回（4级层序）。

The work of this paper is as follows: 1. Looking back the progressing history of the linear motor, introducing the features of the elevator driven by linear induction motor, radicating the topic of this paper "the digital frequency variable control of the elevator bi-side direct driven by linear induction motor". The research of this paper covers the conventional VVVF control, space vector based VVVF control, vector control and DTC.

本文主要开展了以下几个方面的工作： 1 回顾了直线电机发展历史，电机的驱动技术演变，特别是针对直线电机的驱动，简要介绍了直线感应电机驱动电梯的优点和不同结构类型，对传统的v／f控制，基于空间矢量法的v／f控制，矢量控制，和基于电压空间矢量的直接转矩控制进行了比较，确立了本课题的研究主题：直线感应电机双边直推式驱动电梯的全数字变频控制。

The article combines with the treatment of a superficial civil air defense work to introduce how the grouting method to improve the performance of the backfill soil.

文章从治理漂浮人防工事的角度提出了注浆技术在改善回填土性质方面的应用，并详细阐述了注浆技术的施工流程。