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Seven thirdorder seismic sequences can be recognized from bottom to top in the Paleogene in the Wushi sag, which consist of a depositional sequence from transgression to regression as a whole and correspond to different stages of structural evolution. Sequences developed in different stages of structural evolution are different in filling sequence and distribution of sedimentary system. Multiphase structural evolutions result in development of source rocks and reservoir and cap rocks in various parts of the sag. The sequence 2-4 deposited in strong chasmic stage have the best source rocks and sealing conditions as well as various favorable reservoirs, thus are the most favorable targets for obtaining discoveries in this sag.

结果认为：乌石凹陷古近系从下至上可以划分为7个三级地震层序，整体上为一个水进到水退的序列，分别对应不同的构造演化阶段；不同构造演化阶段发育的层序具有不同的充填序列和沉积体系分布；构造演化的多期性使得该凹陷多个部位都有烃源岩和储、盖层发育；其中以强烈裂陷阶段沉积的层序2-层序4中发育的烃源岩和盖层条件为最好且发育多种有利储集体，是该凹陷寻找油气藏的最有利层段。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.After transforming E.coli BL21(DE3) and four hours induction by IPTG,HMGB1 expression confirmed by SDS-PAGE and the purification was performed by Ni2+-chelate affinity chromatograph.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

Methods:The total RNA was extracted from mouse RAW264.7 cells stimulated by LPS.The sequence including the whole length of HMGB1 was amplified by RT-PCR and inserted into pMD-19T.The combinant vector was used as a template for PCR which was cloned into vector pMD-19T,then subcloned into expression vector pET-26b with pelB signal sequence and His-Taq sequence.

脂多糖刺激后的RAW264.7细胞，提取总RNA，经RT-PCR扩增出含HMGB1的目的片段，克隆于pMD-19T载体，再亚克隆至含有pelB引导肽及His-标签肽的高效表达载体pET-26b，转化大肠杆菌BL21（DE3），经IPTG诱导后行SDS-PAGE鉴定目标蛋白表达，用镍鳌合琼脂糖凝胶亲和层析法分离纯化含His-标签肽的目的蛋白。

By comparition on the recognition of normal and sheared DNA sequence, it can be found that, the intercalation at the mismatch site of sheared DNA sequence more specificially than into normal sequence.

但是由于两种手性配合物的插入能量差异不是太大，实验手段可能无法得到满意结果，这可能是导致国际上其他实验工作组研究结果不一致的原因所在；（3）沟选择性比较明显。

The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.

结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达，且在未成熟卵母细胞中，MⅠ期的表达明显强于GV期的表达；②RT-PCR产物克隆测序结果显示：昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp～1153bp)]完全一致；Blastn比对结果发现：在不同物种中差异较小，选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析，提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%，100%，100%，36%，100%，100%，67%，100%，100%，92%，99%，99%，99%；Clustal 1.81和Treeview构建出的分子进化树表明：在上述14种生物中昆明小鼠与中国仓鼠进化关系最近，与兔子、猪聚成一簇，与食蟹猴进化关系最远；③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段，重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。

The sedimentary formation can be summarized as epicontinental sea filling sequence, offshore debris lake basin sequence and inner continental lake basin sequence etc.

在此基础上，对沉积层序地层进行了探讨，划分出了陆表海、近岸碎屑湖盆和内陆湖盆等3个层序组以及18个沉积层序，建立了研究区层序地层学格架。

Given an amino acid sequence, what features should be derived from it and how to formulize these features so as to represent the relationship between the sequence and the structure or function of the corresponding protein correctlyβ This is the problem of characteristic description of the amino acid sequence.

1引言 阐释蛋白质的序列一结构一功能之间的关系是后基因组时代的一项重要任务，蛋白质数据库规模的迅速增长和计算技术的发展为这项任务的研究提供了方便。

Sequence similarity of gyrB sequence from twelve Gordonia type strains ranged from 79.3% to 97.2%, corresponding to 270-41 nucleotide differences while there was only 0.3-3.8% difference in 16S rRNA gene sequence similarity at the interspecies level.

本研究之结果将可利用gyrB基因作为Gordonia菌种快速鉴定之依据，若两菌株之16S rDNA序列相似度大於99.7%，gyrB基因相似度大於97.2%，则判定两菌株为相同菌种。

Gene sequence was highly homologic with a gene encoding aβ- subunit of ATP synthetase and an EST originating from a cDNA in relation to water deficit stress, which both were closely associated with the protein functioning in stress resistance and ion transportation. The results suggested that No. 1 gene sequence was involved in stress resistance of apple rootstock. No. 2 gene sequence had high homology with a gene encoding a serine/ threonine kinase protein, indicating perhaps it was a new gene encoding the serine/ threonine kinase protein in apple rootstock. The gene sequences of A1 and A2 were highly homologic with a gene encoding aβsubunit of ATP synthetase.

对得到的差异片断进行回收、测序，通过BLAST和其它植物比对，发现Ⅰ号序列与一条编码ATP合酶β亚基基因和一条水分亏缺胁迫cDNA克隆基因EST的同源性非常高，该序列编码涉及到了抗性表达、离子转运基因，可能参与了植物抗性表达，说明获得的基因序列应是与苹果砧木抗性相关的基因；Ⅱ序列与一条serine/threonine kinase蛋白激酶的蛋白序列同源性比较高，核酸比对较低，可能是具有丝氨酸/苏氨酸蛋白激酶功能的新基因。A1、A2序列与一条编码ATP合酶β亚基基因的同源性较高。

The rock formation and isochronic sequence stratigraphic correlation framework have been set up which shows the sedimentary sequence in both Luxi and Jiyang depression is similar to each other, being of the same sequence architecture of'duality structure'but a bit differences in sedimentary system.

以多重地层划分及层序地层学理论为指导，运用沉积学、古生物学、地球化学、地球物理学方法和资料对研究区石炭二叠纪陆表海聚煤盆地的沉积层序进行了综合识别分析。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。