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The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨,PBS冲洗髓腔,离心去脂肪及上清液,L-DMEM重悬细胞,沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中,离心后吸取中间界面白膜层,加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基,接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液,去除未贴壁细胞,以后每2 d换液一次。

This article study the condition of column chromatogra-phy for separating the total flavonoids in Bidens pilosa L.by polyamide.

本文以三叶鬼针草为研究对象,采用聚酰胺柱层析法对三叶鬼针草中总黄酮成分的分离条件作了研究。

The invention discloses a dielectric hole-microhole composite material and synthesizing method in the inorganic porous material, adsorbing material and catalytic material technical domain, which comprises the following steps: adopting zeolite molecular sieve as raw material; reacting with sodium bisilicate solution under 30-60 deg.c for 10-300 min; disintegrating particle part of zeolite molecular sieve into piece with microhole structure; adding cetyl trimethyl ammonium bromide as molder; adjusting pH value of mother liquid to 10-12; aging 1-8h; adding in the high-pressure autoclave to do water heat synthesizing reaction at 80-130 deg.c for 24-72h; separating solid; washing; drying; sintering under 500-600 deg.c for 5-10h; obtaining the Si-Al porous material with dielectric hole and microhole composite structure.

本发明属于无机多孔材料、吸附材料和催化材料技术领域,涉及具有介孔-微孔复合材料及其合成方法。其特征是以沸石分子筛为原料,用偏硅酸钠的水溶液在30~60℃反应10~300分钟,使沸石分子筛的颗粒部分解离形成具有微孔结构的碎片,加入模板剂十六烷基三甲基溴化铵,然后调节母液pH值为10~12,老化1~8小时,然后加入高压釜于80~130℃进行水热合成反应。反应24~72小时后,分离出固体,水洗、干燥,然后于500~600℃焙烧5~10小时,得到具有介孔和微孔复合结构的硅铝多孔材料。本发明的效果和益处是所发明的合成方法可用于合成多种沸石结构和介孔结构的复合材料,所合成的材料经过质子交换可以具有较强的酸性,在吸附和催化领域具有广泛应用前景。

Separating acetobacter acetic strains adopted the changing colour circle method with bromocresol purple indicator.

采用溴甲酚紫作为指示剂通过变色圈法,从其中分离醋酸菌。

MethodsUrsolic acid was extracted from Callicarpa macrophylla Vahl leaves by method of ethanol extracting and agglutination separating, and its structure was identified by IR, MS and NMR.

方法采用&醇提凝析法&提取纯化乌索酸,IR、MS、NMR鉴定其结构。

The reason for above results is that only if Ag is in a state of separating particles, can it be an effective capturer of photo-induced electron and reduce the recombination of photo-generated holes and electrons.

原因是这种状态的Ag起到了光生电子捕获剂的作用,减少了光生电子—空穴对的复合几率。

METHODS: On the basis of twocuff technique of Kamada, weve improved the techniques of separating, perfusing donor liver, the shearing and anastomosis of the superior and inferior caval veins, the bile duct anastomosis, and so on.

在Kamada两套袖法基础上,着重对供肝分离、灌注、肝上下腔静脉切取,肝上下腔静脉吻合,胆管吻合等手术方法作了进一步改进。

The most heavy flow ability of barrage swirl is controlled by fastenning device and barrage size, the most heavy discharge is more when barrage aperture is bigger, the influence of barrage position on discharge is not obvious; The pressure of the end of the shaft and fastenning device increases which aroused by barrage, and there is a restraining action to flow waving of the shaft; The barrage aperture is smaller, the increase of pressure is heavier, the influence of position to the increase of pressure is not obvious; The positive pressure of the level section in the hole between fastenning device and barrage increases obviously, the change of press along length reduces, mural press in the hydrosphere separating area of outlet of fastenning device becomes positive pressure, which is propitious to the increase of flow cavitate number in the low pressure area of the outlet of fastenning device; The influence of change of swirl cavum diameter caused by barrage to ventilating state is obvious.

阻塞旋流最大泄流能力由起旋器及阻塞体型共同控制,降低了泄洪洞的泄流量,孔径大的阻塞最大下泄流量较大,阻塞位置对泄流量的影响不明显;阻塞引起竖井及起旋器端头压力增大,对竖井部分的水流波动具有抑制作用;阻塞孔径越小,压力增加越大,位置对压力增大影响不明显;起旋器与阻塞之间水平洞段的止压力明显增加,沿程压力变化减小,起旋器出口的水气分离区壁面压力变为正压力,有利于起旋器出口低压区水流空化数的提高;阻塞引起的旋流空腔直径变化对通气状态产生明显影响。

The primary studies on insecticidical and fungicidical components of endophytic fungus in Celastrus angulatus have been carried out from September, 2000 to June, 2002.The results are as follows: Taking PDA and Czapek as separating culture medium, 39 strains of endophytic fungus were separated from the bark, phloem and xylem of the root and twig of Celastrus angulatus.

作者于2000年9月至2002年6月对杀虫植物苦皮藤中内生真菌的杀虫杀菌活性物质进行了初步的研究,取得了以下结果:采用常规的内生真菌分离方法,以马铃薯葡萄糖培养基、Czapek培养基为分离用培养基,从苦皮藤根和侧枝的表皮、韧皮部和木质部中分离得到39株内生真菌。

By separating the crude oligosaccharide products with various ratios of activated charcoal and Celite, the results indicate that the products from 30% lactose (L30) had an adsorption percentage of 96.3%, the highest one among all mixtures of activated charcoal and Celite. The following fractionation by eluting successively with 10% and 20% ethanol aqueous solutions gave an oligosaccharide yield of 13.5% and a purity of 97.9%.

合成的寡醣以不同比例活性炭和矽藻土吸附分离,结果发现30%乳糖组(L30)经单活性炭组吸附即有96.3 %的寡醣吸附力,是所有活性炭与矽藻土混合组别中最高者,再经10 %与20 %乙醇冲提画分后可得13.5 %的收率,其寡醣纯度更高达97.9 %。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力