查询词典 selenium cell

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

The best selenium-adding time was at 24 hours. When the concentration of sodium selenite was over 12μg/mL it obviously inhibit the conversion of inorganic selenium by yeast. The assimilation rate of selenium by yeast was 62% and the conversion rate was 53%. The cell broken rate with ultrasonic cell pulverizer was about 55%.

啤酒酵母菌的最佳加硒时间为24h，亚硒酸钠浓度大于12μg/mL对啤酒酵母菌转化无机硒有明显抑制作用，啤酒酵母菌对无机硒的摄入率约为62%，转化率约为53%；超生波细胞粉碎仪破碎细胞的破碎率为55%左右。

Paul Nipkow obtains a patent in Germany for TV, using a selenium cell and a mechanical scanning disk.

保罗·尼普可夫获得德国电视发明专利。他在电视技术中采用硒光电管和机械扫描盘。

Some dose of selenium can antagonize the apoptosis and cell cycle of hepatocytes induced by fluoride. Fluoride ; Selenium ; Apoptosis ; Cell cycle

本研究通过培养人肝细胞接触一定剂量氟和硒，探讨氟、硒对人肝细胞凋亡的影响，为进一步探讨氟致肝损伤机理提供有益的资料。1 材料与方法1 。

This is called "Se CD4 T cells out of control"(selenium CD4 T cell tailspin) theory is the AIDS of the University of Georgia, MD, EW Taylor made.

该被称为"硒 CD4 T 细胞失控"（selenium CD4 T cell tailspin）的艾滋病发病理论是佐治亚大学的 E。

Selenium on the role of tumor cells mainly as follows: Se is the destruction of tumor cells; Selenium is blocking tumor cell energy; selenium is the effect of anticancer drugs enhancer. 1999, the Chinese Academy of Agricultural Sciences, the experts in the integration of the Western anti-tumor the essence, the trace element selenium and medicine Xiancao "fungus spores on" organic combination of the successful production of the anti-tumor products combining Chinese and Western - Se Ganoderma Po.

硒对肿瘤细胞的作用主要表现为：硒是肿瘤细胞的杀伤剂；硒是肿瘤细胞的能量阻断剂；硒是抗肿瘤药物的药效增强剂。1999年，中国农业科学院的专家们融合中西方抗肿瘤理论精髓，将微量元素硒与中药仙草"灵芝孢子粉"有机地结合在一起，成功研制出了中西结合抗肿瘤产品———富硒灵芝宝。

Methods: The DNA damage was measured after L-02 cell were incubated with 80μg/ml sodium fluoride, 1.73μg/ml selenium, 80μg/ml sodium fluoride and 1.73μg/ml selenium using Single Cell Electrophosis respectively.

采用80μg/ml氟化钠、1.73μg/ml亚硒酸钠以及80μg/ml氟化钠和1.73μg/ml亚硒酸钠对培养的L-02细胞进行染毒，24小时后用单细胞凝胶电泳技术检测各组L-02细胞DNA损伤情况。

Selenium chitosan could reduce cGMP level, promote Ca(superscript 2+) and cAMP level and cAMP/cGMP ratio, which are all possible mechanisms that selenium chitosan induces NB4 cell apoptosis and inhibite proliferation.

硒化壳聚糖可通过升高细胞钙离子和cAMP含量，降低cGMP含量，增大cAMP/cGMP比值来抑制NB4细胞生长，诱导其凋亡。

Results were shown as followings:(1) Sodium selenite at 0～2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5～2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下：（1）0～2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力；而对SMMC-7721细胞的作用不显著；（2）该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度；而对SMMC-7721细胞的作用均不显著；（3）高浓度硒（5μmol/L以上）显著性抑制L-02细胞生长、致细胞过氧化，灯盏花素能拮抗硒所致过氧化、降低硒毒性；（4）0.5～2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧，同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著；（5）富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白，其结构和功能有待研究；（6）通过已有的含hTERT基因质粒，成功构建hTERT反义RNA表达质粒，转染SMMC-7721细胞后对其生长具有抑制作用。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。