查询词典 selenite

Objective To investigate the effect of different concentrations of sodium arsenite on the reactive oxygen species and lipid peroxidate of the lymphocytes of rats cultured in vitro and the antagonism of sodium selenite and vitamin C.

目的 观察不同浓度的亚砷酸钠(NaAsO2)对体外培养的大鼠淋巴活性氧群和细胞内脂质过氧化物含量的影响，并探讨亚硒酸钠(NaSeO3)和维生素C对砷毒性的拮抗作用。

Methods Vero cells were cultured with mercury chloride, at the same time zinc sulfate, sodium selenite and zinc sulfate together with sodium selenite were added respectively. The tail length and the rate of the comet cells were measured and calculated.

常规培养vero细胞，通过MTT法制定出氯化汞染毒剂量，与氯化汞同时加入不同浓度的硫酸锌、亚硒酸钠以及硫酸锌+亚硒酸钠溶液，共同培养2 h后采用单细胞凝胶电泳法检测细胞的彗星尾长和彗星率。

Result zinc sulfate, sodium selenite and zinc sulfate together with sodium selenite had the effects of antagonism to DNA amage caused by mercury chloride in vero cells.

结果 硫酸锌和亚硒酸钠，以及两者合用都具有拮抗氯化汞致vero细胞DNA损伤的作用，两者最佳组合是1.5 mg/L硫酸锌+1.0μmol/L亚硒酸钠。

Methods Tissue culture was used to study the cataractous lenses of rats induced by sodium selenite or galactose. At the early stage, the content of nonprotein sulfhydryl, protein sulfhydryl, non-soluble disulfide bond, malonaldehyde and the activities of glutathione peroxidase, glutathione reductase and glutathione-S-transferase were measured in the cataractous lenses, and they were compared with that of the normal lenses. Results The two kinds of material could all induce cataract in rats, sodium selenite being more potent.

采用器官培养方法，以不同浓度亚硒酸钠及半乳糖作用于晶状体，诱发大鼠晶状体形成白内障，在培养初期测定晶状体内非蛋白质巯基（nonprotein sulfhydryl， NP-SH、蛋白质巯基（protein sulfhydryl ，P-SH）和不溶性蛋白质二硫键的含量，脂类过氧化水平，以及与谷胱甘肽代谢有关的谷胱甘肽过氧化物酶(GSH peroxidase， GSH-PX)、谷胱甘肽还原酶(GSH reductase， GSSG-R)及谷胱甘肽硫转移酶(GSH S transferase， GSH-S)的活性。

At the experimental concentrations tested (00 u.g I/1), selenite and selenate did not significantly affect the accumulation of either mercury species by the green mussels. In contrast, selenomethionine significantly inhibited the uptake of MeHg and enhanced the uptake of Hgy the mussels, but it did not affect the assimilation from the ingested diatoms. We further examined the influence of tissue body burden of Se in the green mussels following pre-exposure to selenite and selenomethionine on the accumulation of Hg2 and MeHg from the dietary and aqueous phases.

实验结果表明，在实验设定的浓度范围内(00滩。L-l)，同时暴露，s犷和s产均不能显著影响翡翠贻贝刘榕解态和颗粒态H广和MeHg的累积。seMe育游显著抑制翡翠贻贝对溶解态MeHg的吸收，提高对溶解态H犷+的吸收，但不能影响翡翠贻贝对颗粒态Hg的同化效率；通过投喂载反少或女N贻的硅藻，对翡翠贻贝进行预暴露，组织Se的浓度随预暴露时间延长而增加。

The SMMC-7721 hepatoma cell line of experimental groups and control groups were cultured with the addition of sodium selenite, potassium iodide or triiodothyronine (T3). The cell quantity was checked by the MTT assay , and the cell cycle was observed by flow cytometry and levels of AFP and ALB in the culture solution were determined by RIA.The SD rats were divided into positive control group, negative control group, T3 group and mixture group (the mixture of sodium selenite, KI and T3). After two weeks of the administration of sodium selenite、 KI and T3,Walker256 cells were transplanted into the abdominal cavity. The administration was kept on for another two weeks, then observed the morbility and mortality of the rats with ascites carcinoma. At the end, the ascites were for the cells counting and the cell cycles analysising.

方 法：在体外，应用细胞培养的方法，培养SMMC－7721肝癌细胞系，分为试验组和对照组，试验组加入不同浓度Se、I或T3干预，培养一段时间后，通过噻唑蓝试验、流式细胞仪，观察细胞数量及细胞周期的变化，测定细胞培养上清液中甲胎蛋白、白蛋白的水平，观察细胞分化情况；在体内，将试验用SD大鼠分成阴性对照组、阳性对照组、T3组和混合组（为Se、I和T3混合物），首先预防性用药2周，然后给试验组大鼠和阳性对照组大鼠腹腔注射Walker256细胞，制造腹水癌大鼠模型；继续用药2周，观察各组大鼠发病率及死亡率，测量不同时期大鼠血清FT3含量，通过流式细胞仪测量大鼠腹水细胞数及细胞周期变化。

Results When the cells were treated with Sodium Selenite at a concentration of 2.0mmol//L lasting 72h, karyopyknosis and chromatin condensation were observed under microscope; When the cells were treated with Sodium Selenite at a concentration of 4.0mmol/L lasting 72h, the cells in G0/G1 and S phase was significantly decreased, a typical apoptosis peak before G1 phase was shown.

结果 2.0mmol/L亚硒酸钠作用72h， HE切片可见核固缩、染色质边集等改变；4mmol/L亚硒酸钠作用72h， G0/G1期和S期细胞明显减少，在细胞周期G1前期可见典型的细胞凋亡峰。

Methods Mouse were smoking twice everyday in a self-made passive smoking cabinet, medicative group mouse were given respectively with sodium selenite, vitamin E, sodium selenite and vitamin E for 35 days.

方法小鼠置于自制被动吸烟箱中，每日吸烟两次，用药组小鼠分别给予亚硒酸钠、维生素E、亚硒酸钠和维生素E灌胃，连续35d。

Results When the culture medium was supplemented with 0 .05μg/ml sodium selenite,the proliferation of transformed cells was significant ly promoted and the transformation did not change remarkably,when supplemented w ith 0.25μg/ml,the proliferation was promoted but the transformation was inh ibited,and when with 0.75μg/ml and 1.50μg/ml,the proliferation was not pro moted and the transformation was strongly inhibited.Big dose of (1.50μg/ml) sodium selenite m ade the nuclei of the untransformed lymphocytes gather together like a few grape s doing,and even caused them to fuse.

结果 在培养物中添加0.05μg/ml亚硒酸钠能显著促进转化细胞增殖；添加0.25μg/ml可促进转化细胞增殖，但显著抑制细胞转化；添加0.75μg/ml和1.50μg/ml，不仅不能促进细胞增殖，而且强烈抑制细胞转化。1.50μg/ml剂量的亚硒酸钠使非转化淋巴细胞发生葡萄状聚集甚至核融合。

Results were shown as followings:(1) Sodium selenite at 0～2.5 μmol/L significantly increased the antioxidative capacity of L-02 cells without having remarkable impact on SMMC-7721 cells;(2) Sodium selenite at concentrations above significantly increased telomerase activity, hTERT gene expression and telomere length of L-02 cells without significant impact on SMMC-7721 cells;(3) Sodium selenite at higher concentrations (larger than 5 μmol/L) resulted in peroxidation of L-02 cells, while scutellarin significantly counteracted its effect;(4) Selenium-rich amino acids from silkworm pupas in the range of 0.5～2.5 μmol/L Se significantly inhibited SMMC-7721 cell growth, induced apoptosis and cell cycle change, and the generation of reactive oxygen species. In contrast, sodium selenite and selenomethionine only had weak impact on them at the same concentrations;(5) A new selenium-containing protein was found from selenium-rich silkworm pupas, which is worthy to be study further;(6) An expression vector containing ansense RNA of hTERT gene were constructed and used to transfect SMMC-7721 cells. They were observed to inhibit hepatoma cells.

结果如下：（1）0～2.5μmol/L亚硒酸钠显著性增强L-02细胞的抗氧化能力；而对SMMC-7721细胞的作用不显著；（2）该浓度硒显著性提高L-02细胞端粒酶活性、增强hTERT基因表达和延长细胞端粒长度；而对SMMC-7721细胞的作用均不显著；（3）高浓度硒（5μmol/L以上）显著性抑制L-02细胞生长、致细胞过氧化，灯盏花素能拮抗硒所致过氧化、降低硒毒性；（4）0.5～2.5μmol/L富硒蚕蛹氨基酸显著性抑制肝癌细胞SMMC-7721生长、导致细胞凋亡和周期改变、诱导细胞产生活性氧，同浓度亚硒酸钠和硒代蛋氨酸对其抑制不显著；（5）富硒蚕蛹蛋白经分离纯化和鉴定后发现存在一新含硒蛋白，其结构和功能有待研究；（6）通过已有的含hTERT基因质粒，成功构建hTERT反义RNA表达质粒，转染SMMC-7721细胞后对其生长具有抑制作用。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。