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The EAE rats were induced by injecting subcutaneously an emulsion of GPSCH and CFA. In addition, each rat received an intracutaneous injection of pertussis. Albumin in serum and CSF samples was examined on the days of 4,6,8,10,12,14,16,18 and 20 p.i.. The integrity of BBB was assessed by caculating CSF to serum albumin quotient. At the same time the brains, spinal cords, thymus and inguinal lymph nodes were removed for paraffin sections cutting. These sections were used for HE staining and ICAM-1 semiquantitative immunohistochemical staining. The results showed as follows:1. None of EAE rats on days 4,6 and 8 p.i. showed clinical signs whichbecame apparent on day 10 p.i.(2.33±0.76; n=6). On days 12 and 14 p.i.

检测p.i。第4天、6天、8天、10天、12天、14天、16天、18天、20天血清和CSF中ALB含量，其CSF／S ALB比值作为评价BBB损害的指标；同时段取动物脑、脊髓、胸腺和腹股沟淋巴结制成石蜡切片行HE染色和ICAM-1半定量免疫组化分析，结果显示如下： 1、EAE大鼠p.i.4-8天无任何临床表现；p.i.10天症状开始明显，症状评分为2.33±0.76(n=6)；p.i.12-14天为发病高峰期，症状评分为3.75±0.54(n=12)，病情由最初的活动减少，体重减轻逐渐发展为肢体无力、瘫痪，共济失调，甚至濒死状态；p.i.16天大部分动物症状开始缓解，评分为2.08±0.66(n=6)；p.i.20天疾病症状完全消失。

Aortic perfusion of a fixative solution. Coronal sections were cut at 25 μm thickness on a freezing microtome and up to five series of adjacent sections were collected for nNOS immunohistochemical processing using streptavidin????

应用Morris水迷宫测试系统检测大鼠空间参考记忆获得及维持的变化；②海马的nNOS免疫组化研究：PSD 24，48和72 h后，立即取脑、固定、行冰冻切片（片厚25 μm）、SABC法免疫组化染色、光镜下进行观察分析、摄像。

Coronal sections were cut at 25 μm thickness on a freezing microtome and up to five series of adjacent sections were collected for nNOS immunohistochemical processing using streptavidinbiotinperoxidase complex technique.

应用Morris水迷宫测试系统检测大鼠空间参考记忆获得及维持的变化；②海马的nNOS免疫组化探究摘要：PSD 24，48和72 h后，立即取脑、固定、行冰冻切片（片厚25 μm）、SABC法免疫组化染色、光镜下进行观察分析、摄像。

Methods Cryostat sections for adult worms and paraffin sections for eggs in the liver of infected mouse, sporocysts and cercariae in snails were prepared, immunofluorescent test was performed to detect the presence of iNOS in adult worms, sporocysts, cercariae and miracidium, the distribution of this enzyme was observed in different stages of Schistosoma japonicum. Western blotting was used to further demonstrate the immunoreactivity to iNOS in adult worms.

收集日本血吸虫成虫制成冰冻切片，将含胞蚴、尾蚴的感染性钉螺和含成熟虫卵的小鼠肝脏制成石蜡切片，用免疫荧光法检测成虫、胞蚴、尾蚴以及毛蚴中的iNOS，并观察该酶在各期虫体内的分布；用蛋白质印迹法检测成虫匀浆中的可溶性蛋白是否具抗iNOS多抗的可溶性蛋白。

At last, the best sections and orientations which were ensured by the comparable study between the thin sectional anatomy and multiplane TEE were used to diagnose the heart diseases. The best orientations and sections for diagnosing heart diseases were summarized and analyzed.

最后，将薄层断面解剖和多平面TEE对照研究所确立的最佳显示切面和显示方位进行临床验证，即用于诊断心脏各种常见疾病，分析和总结多平面TEE诊断心脏常见疾病的常用方位和最佳显示切面。

One or more plane sections (8,9) and part cylinders (10,11) connecting to the plane sections (8,9) are formed at the outside (7) of the capillary using for fixing the fiber, all the part cylinders (10,11) are formed with a single center axis and the same radius, meanwhile a patchhole (3) of the inserting fixed fiber (2) is formed in the deflected position of the center axis and in the vertical direction of the first plane section (8) which is used as the standard.

在光纤固定用玻璃毛细管(1)的外面(7)形成有一个或多个平面部(8、9)和与该平面部(8、9)连接的一个或多个局部圆筒面(10、11)，该所有的局部圆筒面(10、11)都是以单一的中心轴和相同半径形成的，同时在与作为基准的第一平面部(8)垂直的方向上从中心轴偏离的位置上形成有插入固定光纤(2)的插入孔(3)。

And then the node coordinates of the sections of aircraft can be achieved according to the variable parameters which are to be optimized, the constant parameters and the planform of few control sections.

建立了类似F-22布局的飞机外形的几何模型生成方法，可以根据优化变量和固定参量的值，以及少数几个控制截面的形状，生成飞机外形各个截面的节点坐标。

The results show, when polar distance is bigger than the size of cross section of tunnel, the difference of anomalous feature between the corner-sections and wall-sections in tunnel is very small. The apparent resistivity is only related to the distance between section and bodies. The shielding effect of the tunnel is not so evident that the character and value of anomalous are similar with those in full space.

计算结果表明，当坑道内偶极测深的极距大于坑道截面尺寸时，坑道角与坑道面断面异常形态差异不大；视电阻率值只与断面和地质体间的距离有关；坑道空腔的屏蔽效应并不明显，坑道内各个测深断面的异常幅值和形态与全空间中观测结果非常相似。

Structure-wise, there usually is an introductory section, a fugue and a postlude, but this basic scheme is very frequently expanded: both BuxWV 137 and BuxWV 148 include a full-fledged chaconne along with fugal and toccata-like writing in other sections, BuxWV 141 includes two fugues, sections of imitative counterpoint and parts with chordal writing.

结构明智，通常有一个导言部分，一个赋格和postlude，但这一基本计划是非常频繁地扩大了：既BuxWV 137和BuxWV 148包括一个完整的，以及成熟的舞曲真菌和托卡塔，其他部分一样书面BuxWV 141包括两个赋格曲，对位的模仿与弦写作部分章节。

Paralleling to a principle projective plane Full sections, half-sections and partial sectional views all have a single cutting plane.?

用平行于某一基本投影面的平面剖切？前面介绍的全剖视图、半剖视图和局部剖视图都是单一剖切面剖切的图例。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。