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The results indicated that under the experimental conditions, the highest cell dry weight was 41. 93 g/L; and the highest total protein content, the highest phycocyanin content and the highest secreted extracellular phycocyanin content obtained were 9. 5232 g/L, 61. 195 mg/L and 24. 32 mg/L, respectively.

研究表明在实验条件下所获得的细胞干重最高为41.93g/L，发酵液中蛋白质的最高含量为9.5232g/L，藻蓝蛋白的最高产量为61.195 mg/L，分泌到胞外的藻蓝蛋白的最高产量为24.32 mg／L。

When the environment is too much humidity, the body of the pineal hormone secreted in large amount, making the body the concentration of thyroxine and adrenaline on the relative reduction would feel listless, dispirited.

当环境湿度过大时，人体中分泌出的松果激素量也较大，使得体内甲状腺素及肾上腺素的浓度就相对降低，人就会感到无精打采，萎靡不振。

As we age, the amount of HGH secreted by our body's pituitary gland decreases, resulting in the symptoms of aging you see in your skin (wrinkles, loss of tone and firmness), energy level, libido, hair color (loss of hair color, or graying of hair), weight gain, and muscle to fat ratio.

我们的时代，我们的身体分泌生长激素量的脑垂体跌幅造成的症状你看看你的皮肤老化能级，性欲、头发颜色、体重增加、肌肉脂肪比率。

Human Growth Factor is a natural hormone secreted by the pituitary gland.

人类生长因子是一种促进脑垂体分泌的生长素荷尔蒙的物质。

Background: Interleukin-4 is a pleiotropic cytokine secreted by Th2 cells and mast cells.

背景：IL-4是一种具有多种功能的Th2细胞因子，它与IL-13二者在支气管哮喘的炎症方面有重要的作用。

Methods CD+4CD45RO+ T lymphocyte subsets were isolated from peripheral blood of patients with bronchial asthma and healthy donors. Then they were cultured with self-B lymphocytes and designated as pokeweed mitogen-stimulated and non-stimulated group. Secreted IgE was measured in the supernatant.

分别分离出支气管哮喘病人及健康对照的CD+4CD45RO+T淋巴细胞亚群，并将其与各自的B淋巴细胞共同培养，设定刺激组及非刺激组，测定培养上清液中IgE的含量。

The total RNA was isolated from pokeweed leaves using the method of guanidine isothiocyante and used as template for amplification of the deleted mutant pokeweed antiviral protein gene by RT-PCR and then the amplified fragment was cloned into secreted expression pPIC9K vector to form pPIC9K-p, then the vector was transferred into Pachia pastoris GS115 strain.

从美洲商陆叶片中通过异硫氰酸胍法提取出了总RNA，经RT-PCR扩增出缺失突变型抗病毒蛋白PAP基因，将该基因克隆于分泌型真核表达载体pPIC9K，然后导入毕赤酵母菌株GS115细胞。

Transgenic tobacco plants were obtained through screening with kanamycin. The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants. Pokeweed antiviral protein Ⅱ is expressed with high level in summer leaves. The expression of PAPⅡ is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPⅡ gene by RT-PCR and then the gene was cloned into E. coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPⅡ gene were then transferred into E. coli strain BL21 (DE3)-plysS and Pachia pastoris GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陆抗病毒蛋白Ⅱ是一个受季节调控表达的蛋白，本实验以美洲商陆夏季叶片为材料，通过对其总RNA的提取、反转录、并用PAPⅡ的特异引物进行PCR扩增，对PAPⅡ进行了基因克隆、序列分析，结果扩增出来的PAPⅡ基因与已经报道的序列同源性是99.9％，然后将该基因构建到原核、真核表达载体上，分别转化了大肠杆菌和毕赤酵母并对它们进行了诱导表达，在两个表达系统中均获得了有活性的PAPⅡ表达蛋白，体外生物测定表明表达的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中还没有表达的报道，这为获得具活性PAPⅡ蛋白提供了一种简便可行的方法。

Polyarthritis model was induced by Freund's complete adjuvant in rats. The edema degree of the inflamed paws general affection T lymphocyte proliferation function, IL-1 activity secreted by celiac macrophage, peroxide lipid and SOD-1 in serum were detected.

用弗氏完全佐剂造成大鼠多发性关节炎，观察足跖肿胀程度、全身病变、脾脏T淋巴细胞增殖功能、腹腔巨噬细胞白介素 1(IL-1)分泌活性、血清脂质过氧化和超氧化物歧化酶(SOD-1)活性。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新